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Peach SSAP (Source Service Access Point) molecular marker primer combination, molecular marker combination and application of molecular marker combination in analysis on genetic diversity of peach varieties

A primer combination and molecular marker technology, applied in the field of molecular biology, can solve the problems of large heterogeneity of retrotransposon primers and no reports on the application of SSAP molecular marker technology for genetic diversity analysis.

Active Publication Date: 2014-08-06
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, SSAP molecular marker technology has been successfully used in the genetic diversity analysis of grape, barley, apple and other species (Labra, 2004; Queen, 2004; Venturi, 2006), but the development of retrotransposon primers is race-specific, and different species There is great heterogeneity among retrotransposon primers, so far there is no report on the application of SSAP molecular marker technology in the analysis of genetic diversity in peach cultivars

Method used

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  • Peach SSAP (Source Service Access Point) molecular marker primer combination, molecular marker combination and application of molecular marker combination in analysis on genetic diversity of peach varieties
  • Peach SSAP (Source Service Access Point) molecular marker primer combination, molecular marker combination and application of molecular marker combination in analysis on genetic diversity of peach varieties
  • Peach SSAP (Source Service Access Point) molecular marker primer combination, molecular marker combination and application of molecular marker combination in analysis on genetic diversity of peach varieties

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Experimental program
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Effect test

Embodiment 1

[0065] The application of SSAP molecular markers in peach variety genetic diversity analysis, its steps include:

[0066] (1) Primer design and synthesis: The entire genome sequence of peach was analyzed using the retrotransposon prediction software LTR_STRUC version 1.1. In the predicted result data, retrotransposons with a similarity of more than 99% of the LTR sequences at both ends were preferentially selected, and the downstream primers required for selective amplification in the SSAP molecular marker were designed using Primer Premier 5.0 and referring to the sequence of the 3' LTR region. When designing primers, only choose to start from the first base at the 5' end of the region, and replace the first base with a mismatched base. The length of the primer is designed to be 19-23bp, and three Selective bases are GAG, CGT and GGT to form different primer combinations. At the same time, 18bp universal primer sequence (M13) TGTAAAACGACGGCCAGT is added to the 5' end of T...

Embodiment 2

[0089] The application of 10 SSAP molecular marker combinations in the genetic diversity analysis of 8 ornamental peach varieties, the steps include:

[0090] (1) Primer synthesis: All the primers shown in Table 1 were synthesized.

[0091] Table 3 Information about the characteristics of 8 ornamental peach varieties used in Example 2

[0092] serial number Variety branch type petal color 1 Jinling Jintao Straight branch type Variegated 2 white peach Straight branch type White 3 Pretty in Pink Straight branch type pink 4 Human face peach Straight branch type pink 5 red leaf peach Straight branch type red 6 five treasures Straight branch type Variegated 7 mandarin duck weeping branch Weeping type Variegated 8 sprinkle red peach Straight branch type Variegated

[0093] (2) 8 ornamental peach varieties (see Table 3 for the characteristic information of the 8 ornamenta...

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Abstract

The invention discloses a peach SSAP (Source Service Access Point) molecular marker primer combination, a peach SSAP molecular marker combination and an application of the peach SSAP molecular marker combination in analysis on the genetic diversity of peach varieties, wherein the peach SSAP molecular marker primer combination comprises an LTR (Long Terminal Repeat) primer, a selective amplification primer and a tail primer; the peach SSAP molecular marker combination comprises ten molecular markers JY01, JY02, JY03, JY04, JY05, JY06, JY07, JY08, JY09 and JY10. Due to the design of a peach retrotransposon LTR sequence primer, a selective amplification product is proved to be clear and abundant in amplification strip and have high efficiency, reliability and practicability through fluorescent capillary electrophoresis detection; in addition, a selective amplification PCR (Polymerase Chain Reaction) system is optimized, and a tail sequence is added, so that the traditional selective amplification PCR system is improved, and the cost for carrying out relevant researches by using the molecular markers is reduced. The SSAP molecular marker combination disclosed by the invention has relatively high polymorphism in the plurality of peach varieties, comprises stably existing markers and can be used for peach variety identification and genetic diversity analysis.

Description

technical field [0001] The invention relates to a primer combination of peach SSAP molecular markers, a combination of peach SSAP molecular markers and the application thereof in the analysis of genetic diversity of peach varieties, belonging to the field of molecular biology. Background technique [0002] Peach[ Prunus persica (L.) Batsch] belongs to the Rosaceae ( Rosaceae ), Prunus ( Prunus L.), rich in germplasm resources, originated in western my country. According to statistics, my country has the most abundant peach germplasm resources in the world, and various varieties and excellent lines have been accumulated through natural variation and artificial breeding. There are more than 1,600 peach germplasm resources preserved by the Zhengzhou Fruit Tree Research Institute of the Academy of Agricultural Sciences and the Horticultural Research Institute of the Jiangsu Academy of Agricultural Sciences. [0003] A large number of studies have shown that my country's p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 焦云马瑞娟俞明亮沈志军
Owner JIANGSU ACAD OF AGRI SCI
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