Molecular marker for japonica rice genetic diversity analysis and authentication method for molecular marker

A genetic diversity and molecular marker technology, applied in the field of detection in rice breeding, can solve the problems of small genetic differences and difficult selection differences in japonica rice varieties.

Inactive Publication Date: 2014-05-14
FARMING & CULTIVATION RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI
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Problems solved by technology

In addition, japonica rice has been favored by more and more people because of its better taste than indica rice. However, the small genetic differences in japonica rice varieties make it more difficult to select japonica rice with large differences as parents to obtain larger hybrids with high quality.

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  • Molecular marker for japonica rice genetic diversity analysis and authentication method for molecular marker
  • Molecular marker for japonica rice genetic diversity analysis and authentication method for molecular marker
  • Molecular marker for japonica rice genetic diversity analysis and authentication method for molecular marker

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[0041] The above and other technical features and advantages of the present invention will be described in more detail below in conjunction with the accompanying drawings.

[0042] The present invention adopts the Japanese japonica rice varieties Ichimoku, Fengjin, Sajinishi, Nipponbare, and Akita Komachi with close genetic relationship; Shennong 265, Liaojing 263, and Liaojing 294 introduced into the Northeast rice region with a small amount of indica blood; and wide affinity japonica rice 02428 was used as material, and 32 pairs of SSR primers suitable for the analysis of genetic diversity in japonica rice were determined by screening 489 pairs of molecular markers evenly distributed on 12 rice chromosomes, with PIC value >0.50 as the main reference index.

[0043] These primers can well reflect the genetic diversity and genetic differences among these japonica rice materials, and can be used for large-scale evaluation of japonica rice variety resources and screening of breed...

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Abstract

The invention relates to a molecular marker for japonica rice genetic diversity analysis and an authentication method for the molecular marker. The molecular marker comprises 32 pairs of SSR markers covering 12 chromosomes of paddy rice; each of the fourth, sixth and ninth chromosomes has one marker, each of the fifth, seventh, eighth and tenth chromosomes has two markers; each of the second, third and twelfth chromosomes has three markers; and each of the first and eleventh chromosomes has six markers. The authentication method comprises the steps of a, DNA extraction; b, PCR (polymerase chain reaction) amplification: a PCR reaction system is 15 microlitres and contains 50mM of KCl, 10mM of Tris-HCl(pH8.8), 0.1 percent of Triton-X, 1.5mM of MgCl2, 200mu M of each of NTPs, 0.2mu M of each primer, 5 percent (v/v) dimethyl sulfoxide and 0.5UT of aq DNA polymerase; c, PCR product detection; and d, statistical analysis. According to the molecular marker for japonica rice genetic diversity analysis and the authentication method for the molecular marker, the genetic diversity of a japonica rice material to be detected and genetic difference can be detected, and the molecular markers for the japonica rice genetic diversity analysis are screened.

Description

technical field [0001] The invention relates to the technical field of detection in rice breeding, in particular to the evaluation of the variety resource diversity of japonica rice and the detection of parental genetic differences in molecular assisted breeding of japonica rice. Background technique [0002] Both the genetic improvement of rice and the selection of breakthrough varieties mainly depend on the mastery and utilization of germplasm resources. Only by knowing the genetic variation information of breeding materials can we select parents in a targeted manner, obtain greater heterosis in offspring, and cultivate excellent varieties. Therefore, the analysis of parental genetic diversity is of great significance for the breeding of new rice varieties. [0003] In recent years, since the whole genome sequencing of rice has been completed, on this basis, favorable genes have been cut, aggregated and improved, and breeding has been designed to cultivate rice with stron...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 姜树坤徐正进张凤鸣王嘉宇白良明孙世臣丁国华王彤彤张喜娟姜辉
Owner FARMING & CULTIVATION RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI
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