SSR primer group developed on basis of zucchini transcriptome sequence and application of SSR primer group

Abstract

The invention discloses an SSR primer group developed on the basis of a zucchini transcriptome sequence and application of the SSR primer group in identification of a genetic relationship and a hereditary character of zucchini, and belongs to the technical field of molecular biology. The SSR primer group comprises 35 pairs of primers, and nucleotide sequences of the first pair of primers to the 35th pair of primers are as shown in SEQ ID No. 1 to SEQ ID No. 70. The obtained SSR primer group has the advantages of abundant polymorphism, good repeatability, stable amplification, clear electrophoretic bands and the like. Meanwhile, because the primers are from zucchini expression genes and can reflect variation of a functional domain in a genome, diversity of genetic germplasm of loofah can be distinguished well. The primer group can be used for zucchini material resource genetic diversity analysis, genetic relationship identification, DNA finger-print construction and the like.

Description

technical field [0001] The invention specifically relates to an SSR primer set developed based on the sequence of the American pumpkin transcriptome and its application, and belongs to the field of biotechnology. Background technique [0002] Pumpkin ( Cucubita pepo L. ) is an annual vine herb of Cucurbitaceae Cucurbitaceae, and one of the earliest cultivated crops by humans. It is delicious, nutritious, and has medicinal value and is loved by the public. In the production of American squash, it has strong adaptability. The planting cycle is short, the cultivation is relatively easy, the product yield is high, the supply cycle is very long, and it is also relatively easy to store and is suitable for intercropping. It has a wide range of planting in my country and has become one of the main varieties of melon vegetables in my country. First, the cultivated area is second to cucumber. The development time of American squash breeding in my country is relatively short, and t...

AI Technical Summary

Problems solved by technology

At present, there are very few SSR reports on Squash squash, and the number of molecular markers affects the accuracy of genetic diversity analysis of germplasm resources and the accuracy of genetic map construction. Therefore, the development of a large number of SSR markers is still one of the important tasks in the current study of American squash.

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