ssr primer set and its application based on millet dus testing standard varieties

A technology for testing standards and standard varieties, applied in the field of analytical biology, can solve problems such as long cycle time and test results easily affected by environmental factors, achieve uniform amplification, improve the level of genetic breeding of millet, and amplify bands clear effect

Inactive Publication Date: 2019-11-26
HEBEI NORMAL UNIV FOR NATTIES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the internationally accepted DUS test mainly uses the apparent morphological characteristics of plants as indicators, and its test results are easily affected by environmental factors, and the cycle is long
With the narrowing of the genetic basis of breeding materials and the increasing number of breeding varieties, the differences between varieties are getting smaller and smaller, and it is difficult to accurately identify them solely by traditional botanical characters.

Method used

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  • ssr primer set and its application based on millet dus testing standard varieties
  • ssr primer set and its application based on millet dus testing standard varieties
  • ssr primer set and its application based on millet dus testing standard varieties

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Embodiment Construction

[0038] Based on the SSR primer set of DUS standard varieties of millet, the SSR fingerprints of 32 DUS standard millet varieties formed by using 20 pairs of primers and the DNA of 32 DUS standard varieties of millet through amplification and electrophoresis detection, the 20 pairs of primers The nucleotide sequence is: SEQ ID NO.1~SEQ ID NO.40, as shown in the sequence listing (table 1), the Name of SSR marker ( Primer name), Primer sequence (primer sequence number), Chromosoe located (chromosomal position), Tm (°C) (annealing temperature), Expected size (bp) (primer size); the 32 DUS standard varieties are VAR ID NO. 1 Lianggu, VAR ID NO.2 Zhangai 10, VAR ID NO.3 Yugu No. 8, VAR ID NO.4 Hongmiaoqing, VAR ID NO.5 Yegu No. 5, VAR ID NO.6 Japan Chixu, VAR ID NO.7 Jinmiaogu, VAR ID NO.8 Ukraine, VAR ID NO.9W67, VAR ID NO.10 Red Tenlixiang, VAR ID NO.11 Peach Blossom Rice, VAR ID NO.12 Red Powder Valley, VAR ID NO.13 Yan'an Large Grain , VAR ID NO.14 Cloudy and Drought, VAR ID NO...

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Abstract

The invention discloses an SSR (simple sequence repeats) fingerprint spectrum developed on the basis of millet DSU (distinctness, uniformity and stability) testing standard varieties and application of the SSR fingerprint spectrum, and belongs to the technical field of molecular biology. The SSR fingerprint spectrum comprises 20 pairs of primers and 32 standard varieties. The nucleotide sequences of the primers are shown as SEQ ID NO.1-20 of a sequence table, and the standard varieties are shown as VAR ID NO. 1-32 of a variety table. The primers are amplified in the 32 standard varieties uniformly and stably, amplification bands are clear, and rich polymorphism is achieved. The application of the SSR fingerprint spectrum in millet variety identification is also provided. By the SSR fingerprint spectrum, reliable evidence can be provided for millet variety ownership disputes.

Description

technical field [0001] The invention relates to a millet SSR primer set, in particular to an SSR fingerprint developed based on the SSR primer set of a millet DUS test standard variety and an application thereof, belonging to the technical field of analytical biology. Background technique [0002] Millet (Setaria italica L.Beauv.) belongs to Poaceae, Setaria (L.) Beauv.], self-pollinated, is a very important food and forage crops, widely planted in China, Japan, India, The arid and semi-arid areas of Pakistan and other countries, the Korean Peninsula in East Asia, Mongolia and Ukraine also have stable cultivation areas, and there are also a small amount of cultivation in other areas. It has strong drought resistance and water saving, and high water utilization rate. , Strong drought tolerance, strong stress resistance, wide adaptability and relatively stable yield. [0003] Millet is a C4 plant with the characteristics of high light and action efficiency. It is a new model ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/156
Inventor 王春芳李伟刘国庆张温典刁现民智慧
Owner HEBEI NORMAL UNIV FOR NATTIES
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