ssr primer set and its application based on millet dus testing standard varieties
A technology for testing standards and standard varieties, applied in the field of analytical biology, can solve problems such as long cycle time and test results easily affected by environmental factors, achieve uniform amplification, improve the level of genetic breeding of millet, and amplify bands clear effect
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[0038] Based on the SSR primer set of DUS standard varieties of millet, the SSR fingerprints of 32 DUS standard millet varieties formed by using 20 pairs of primers and the DNA of 32 DUS standard varieties of millet through amplification and electrophoresis detection, the 20 pairs of primers The nucleotide sequence is: SEQ ID NO.1~SEQ ID NO.40, as shown in the sequence listing (table 1), the Name of SSR marker ( Primer name), Primer sequence (primer sequence number), Chromosoe located (chromosomal position), Tm (°C) (annealing temperature), Expected size (bp) (primer size); the 32 DUS standard varieties are VAR ID NO. 1 Lianggu, VAR ID NO.2 Zhangai 10, VAR ID NO.3 Yugu No. 8, VAR ID NO.4 Hongmiaoqing, VAR ID NO.5 Yegu No. 5, VAR ID NO.6 Japan Chixu, VAR ID NO.7 Jinmiaogu, VAR ID NO.8 Ukraine, VAR ID NO.9W67, VAR ID NO.10 Red Tenlixiang, VAR ID NO.11 Peach Blossom Rice, VAR ID NO.12 Red Powder Valley, VAR ID NO.13 Yan'an Large Grain , VAR ID NO.14 Cloudy and Drought, VAR ID NO...
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