7 results about "Setaria" patented technology

The application provides application of a CAPS molecular marker GW18 in identification of foxtail millet (Setaria italica) resistance to rice blast, and belongs to the technical field of molecular marker breeding. The application provides the application of the CAPS molecular marker GW18 in identification of the foxtail millet resistance to the rice blast, and G / T polymorphism exists at 34523782 bp of the 6th chromosome of the foxtail millet; when the polymorphism is G, the resistance to the rice blast cannot be recognized by a restriction enzyme, and is identified as the susceptibility to the rice blast; when the polymorphism site is T, the GW18 target sequence can be recognized by the restriction enzyme and is cut into two segments, and is identified as the resistance to the rice blast. The marker has a high co-separation rate with the disease resistance, a rapid, accurate and low-cost detection method, and can be used for rapid identification of foxtail millet germplasm resources resistant to the rice blast and molecular marker assisted breeding, and has important significance for improving breeding efficiency and guaranteeing safe production of the foxtail millet.

The invention discloses a function and application of SiDRe1 for regulating and controlling the drought resistance of millet, and aims at solving the technical problem of how to improve the drought resistance of the millet. Specifically disclosed are applications of a protein, a substance for knocking out or inhibiting or reducing or down-regulating expression of a coding gene of the protein, or a substance for knocking out or inhibiting or reducing or down-regulating activity or content of the protein in any one of the following: A1) an application in improving drought resistance of green bristlegrass plants; a2) application in preparation of products for improving drought resistance of green bristlegrass plants; a4) plant breeding and / or preparation of plant breeding products; the protein is any one of the following: B1) a protein with an amino acid sequence as shown in SEQ ID No.3; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the protein B1), has 80% or more of identity with the protein B1) and has the same function as the protein B1); and B3) a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The drought resistance of the millet can be improved by inhibiting or reducing or down-regulating the expression of the GmPHD6 gene of the millet, and the GmPHD6 gene can be used for production.

The invention discloses application of a CLE11 small peptide gene in regulation and control of plant salt tolerance, and belongs to the technical field of biological materials. According to the invention, foxtail millet is used as a research material, a SiPROCLE11 gene (CLE11 small peptide gene) is cloned, a SiPROCLE11 overexpression and silent foxtail millet material is constructed, an artificially synthesized small peptide SiCLE11p is externally applied, and the effect of the small peptide SiCLE11p on enhancing the salt tolerance of foxtail millet is determined. Furthermore, it is found that the Setaria italica can promote lateral root development of Setaria italica and reduce Na < + > accumulation in root systems, and a foundation is laid for digging of salt-tolerant related genes of Setaria italica and salt-tolerant breeding of

The invention discloses application of a millet plant type regulation gene. Plant types comprise plant height and / or tillering. The SiDT1 gene (the nucleotide sequence is shown as Seq ID No: 1) is cloned by utilizing a dwarf tillering millet mutant through a map-based cloning method, the function of the gene is verified through transgene complementation, the gene encodes a gibberellin 3 oxidase (the amino acid sequence is shown as Seq ID No: 2), and the characters of plant height, tillering and the like are influenced by influencing synthesis of gibberellin active substances. The invention also discloses a mutant gene of the gene, a protein coded by the mutant gene, a plasmid containing the mutant gene, a plant expression vector, a host cell and application of the mutant gene in regulation and control of plant types. The invention also discloses an application of a mutant containing the mutant gene in regulation and control of the millet plant type, and discloses an application of a molecular marker closely linked with the millet plant type regulation and control gene. The invention provides a new thought for shaping a new variety of ideal plant types of plants.

The present invention discloses a method for planting Setaria viridis in the arid areas of Northwest China. The method belongs to the field of agricultural planting. The method comprises the following steps: planting in an area below 1800m above sea level to increase the seed setting rate and maturity; selecting a deep and flat soil layer, and adopting measures such as applying base fertilizer and irrigating before sowing to accelerate the emergence of seedlings and improve the survival rate; screening seeds by water sorting to improve the seed purity and germination rate; sowing in an area with an air temperature of above 20°C and a soil moisture content of 30% to 40% after irrigating for 5 days to increase the emergence rate of Setaria viridis; adopting a fixed and quantitative drip irrigation and topdressing to achieve water-saving and efficient irrigation and nutrient supplementation; and timely mowing and harvesting according to the growth characteristics of the plant to increase the biological yield and seed quality of Setaria viridis. The method improves the production efficiency of Setaria viridis, accelerates the grassland establishment process of Setaria viridis, and provides technical support for ensuring the promotion and utilization of Setaria viridis in the arid areas of Northwest China.

The invention discloses diphosphonic acid and a salt compound thereof, a preparation method and application. The structural formula of the bisphosphonic acid and the salt compound thereof is shown as a formula (I). According to the preparation method, methyl (3-oxopropyl) phosphinic acid is taken as an initial raw material, a target product [1-hydroxy-3-(methylphosphonyl) propyl]-methylphosphinic acid is prepared through condensation and hydrolysis two-step reaction, and the target product reacts with an alkaline substance to obtain corresponding salt. The obtained target product has a bisphosphonic acid skeleton, and the bisphosphonic acid herbicide lead compound with a novel structure is beneficial to improving the weed removal effect; meanwhile, the target product is salified to obtain a salt compound, the salt compound is used for preparing herbicides for preventing and controlling green bristlegrass, amaranthus retroflexus, chenopodium album and other weeds, the obvious weeding effect is achieved, the water solubility, hygroscopicity, stability and the like of the compound can be changed by preparing the salt compound into the salt, and therefore different preparation requirements are met.

The application discloses a kind of bisphosphonic acid and its salt compound, preparation method and application.The structural formula of the bisphosphonic acid and its salt compound is as shown in formula (I).The preparation method is to prepare target product [1-hydroxy-3-(methyl phosphine acyl) propyl]-methyl hypophosphorous acid by condensation and hydrolysis two-step reaction with methyl (3-oxo propyl) hypophosphorous acid as starting material, and the target product is reacted with basic substance to obtain corresponding salt.The target product obtained by the application has bisphosphonic acid skeleton, and the novel bisphosphonic acid herbicide lead compound is beneficial to improve weed removal effect;Meanwhile, the salt compound obtained by salifying the target product is used to prepare herbicide for preventing and treating setaria viridis, amaranthus retroflexus, small gooseweed and other weeds, and has obvious weeding effect, and preparing salt can improve water solubility, hygroscopicity, stability and the like of the compound, so as to adapt to different formulation requirements.