15 results about "Setaria" patented technology

To provide a new composition consisting of 100% natural matter, exerting a therapeutic or beneficial effect in the treatment of bone fragility, having a physiological mode of action.SOLUTION: The present invention provides a 100% natural product consisting of natural matrices exhibiting emerging properties, which enables re-establishing correct bone metabolism by correcting behavior of mesenchymal stem cells and re-establishing a correct balance between cell populations that allow bone growth or remodeling. The natural product consists of, for example, Equisetum flowering tops, Acacia, Malpighia fruit, coral skeleton, avian eggshell, Agave leaves, Cetraria stems, Agaricus, and calcium citrate.SELECTED DRAWING: None

The invention discloses an application of a millet SiPYL3 gene and an encoding protein thereof in regulation and control of plant stress resistance and / or yield. The invention relates to the field of plant genetic engineering, and provides application of SiPYL3 protein or a coding gene thereof in regulation and control of plant salt resistance and / or drought resistance and / or yield. The amino acid sequence of the SiPYL3 protein is as shown in SEQ ID No. 1. According to the invention, the coding gene of the SiPYL3 protein from millet is introduced into rice, so that the salt tolerance of the obtained transgenic line is improved, the drought resistance is reduced, and the yield is increased. Therefore, the SiPYL3 gene can positively regulate the salt tolerance and yield traits of the plant and negatively regulate the drought resistance of the plant. The invention has important significance for cultivating salt-resistant and high-yield plant varieties.

The invention discloses a PCR (Polymerase Chain Reaction) detection specific primer for rhizoctonia solani AG4HG-III (Ribonia solani AG4HG-III) and a corresponding detection method of the rhizoctonia solani AG4HG-III (Ribonia solani AG4HG-III). A pair of PCR specific amplification primers is designed according to an ITS (internal transcribed spacer) gene sequence of the millet rhizoctonia solani AG4HG-III: an upstream primer wk-15 '-GGTATTCCTTGGAGCATGCCTG-3', a downstream primer wk-15 '-GGTATTCCTGCCTG-3', a downstream primer wk-15 '- And a downstream primer wk-25 '-TGGCCACCCTCCCAAACTC-3' is shown in the description. Through PCR (polymerase chain reaction) amplification, a specific 232bp band can be amplified in the millet rhizoctonia solani AG4HG-III pathogen and a millet diseased plant infected by the millet rhizoctonia solani AG4HG-III. The detection method provided by the invention can be applied to detection of the millet rhizoctonia solani AG4HG-III in millet plants in millet production, provides early warning for distribution and occurrence of the millet rhizoctonia solani AG4HG-III in a millet field, provides a theoretical basis for prevention and treatment and diagnosis of the millet rhizoctonia solani, and has a very good application prospect.

The application provides application of a CAPS molecular marker GW18 in identification of foxtail millet (Setaria italica) resistance to rice blast, and belongs to the technical field of molecular marker breeding. The application provides the application of the CAPS molecular marker GW18 in identification of the foxtail millet resistance to the rice blast, and G / T polymorphism exists at 34523782 bp of the 6th chromosome of the foxtail millet; when the polymorphism is G, the resistance to the rice blast cannot be recognized by a restriction enzyme, and is identified as the susceptibility to the rice blast; when the polymorphism site is T, the GW18 target sequence can be recognized by the restriction enzyme and is cut into two segments, and is identified as the resistance to the rice blast. The marker has a high co-separation rate with the disease resistance, a rapid, accurate and low-cost detection method, and can be used for rapid identification of foxtail millet germplasm resources resistant to the rice blast and molecular marker assisted breeding, and has important significance for improving breeding efficiency and guaranteeing safe production of the foxtail millet.

The present invention provides a method for detecting the insertion copy number of foreign genes in Setaria viridis or foxtail millet by fluorescence quantitative PCR or digital PCR, belonging to the field of biotechnology. The method uses RA1 gene as an internal reference gene and Hyg screening marker gene as a foreign gene, and uses primer pairs RA1-F / RA1-R and primer pairs Hyg-F / Hyg-R to detect the genomic DNA of transgenic Setaria viridis or transgenic foxtail millet, and determine the insertion copy number of foreign genes in transgenic Setaria viridis or transgenic foxtail millet. The present invention provides a method for detecting the insertion copy number of foreign genes in Setaria viridis or foxtail millet by fluorescence quantitative PCR or digital PCR, which has the characteristics of simplicity, rapidity, low sample consumption, high efficiency, high throughput, etc., can improve the efficiency of screening transgenic lines of Setaria viridis or foxtail millet with low-copy insertions, and provides a new option for the detection method of the insertion copy number of foreign genes in the research of transgenic Setaria viridis or transgenic foxtail millet.

The invention discloses a function and application of SiDRe1 for regulating and controlling the drought resistance of millet, and aims at solving the technical problem of how to improve the drought resistance of the millet. Specifically disclosed are applications of a protein, a substance for knocking out or inhibiting or reducing or down-regulating expression of a coding gene of the protein, or a substance for knocking out or inhibiting or reducing or down-regulating activity or content of the protein in any one of the following: A1) an application in improving drought resistance of green bristlegrass plants; a2) application in preparation of products for improving drought resistance of green bristlegrass plants; a4) plant breeding and / or preparation of plant breeding products; the protein is any one of the following: B1) a protein with an amino acid sequence as shown in SEQ ID No.3; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the protein B1), has 80% or more of identity with the protein B1) and has the same function as the protein B1); and B3) a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The drought resistance of the millet can be improved by inhibiting or reducing or down-regulating the expression of the GmPHD6 gene of the millet, and the GmPHD6 gene can be used for production.

The invention discloses application of a CLE11 small peptide gene in regulation and control of plant salt tolerance, and belongs to the technical field of biological materials. According to the invention, foxtail millet is used as a research material, a SiPROCLE11 gene (CLE11 small peptide gene) is cloned, a SiPROCLE11 overexpression and silent foxtail millet material is constructed, an artificially synthesized small peptide SiCLE11p is externally applied, and the effect of the small peptide SiCLE11p on enhancing the salt tolerance of foxtail millet is determined. Furthermore, it is found that the Setaria italica can promote lateral root development of Setaria italica and reduce Na < + > accumulation in root systems, and a foundation is laid for digging of salt-tolerant related genes of Setaria italica and salt-tolerant breeding of

The invention discloses application of a millet plant type regulation gene. Plant types comprise plant height and / or tillering. The SiDT1 gene (the nucleotide sequence is shown as Seq ID No: 1) is cloned by utilizing a dwarf tillering millet mutant through a map-based cloning method, the function of the gene is verified through transgene complementation, the gene encodes a gibberellin 3 oxidase (the amino acid sequence is shown as Seq ID No: 2), and the characters of plant height, tillering and the like are influenced by influencing synthesis of gibberellin active substances. The invention also discloses a mutant gene of the gene, a protein coded by the mutant gene, a plasmid containing the mutant gene, a plant expression vector, a host cell and application of the mutant gene in regulation and control of plant types. The invention also discloses an application of a mutant containing the mutant gene in regulation and control of the millet plant type, and discloses an application of a molecular marker closely linked with the millet plant type regulation and control gene. The invention provides a new thought for shaping a new variety of ideal plant types of plants.

The invention discloses a ZmRF1 gene and application of the ZmRF1 gene in regulation and control of corn fox tail mosaic virus resistance, and provides an SNP (Single Nucleotide Polymorphism) marker as a functional molecular marker. The nucleotide sequence of the disease-resistant allele is shown as SEQ ID No: 1 in a sequence table. The resistance allele encodes the complete ZmRF1 protein, and a single nucleotide polymorphism site (SNP: B73 fourth version genome Chr5218585665) which is changed from C to T exists in a coding region of the susceptible allele, so that an advanced termination codon is generated in the coding region of the ZmRF1, and then 244 amino acids at the C terminal of the ZmRF1 protein are deleted to generate a susceptible phenotype. Therefore, the Chr5218585665 site is a functional marker site and can be used for molecular marker breeding.

The present invention discloses a method for planting Setaria viridis in the arid areas of Northwest China. The method belongs to the field of agricultural planting. The method comprises the following steps: planting in an area below 1800m above sea level to increase the seed setting rate and maturity; selecting a deep and flat soil layer, and adopting measures such as applying base fertilizer and irrigating before sowing to accelerate the emergence of seedlings and improve the survival rate; screening seeds by water sorting to improve the seed purity and germination rate; sowing in an area with an air temperature of above 20°C and a soil moisture content of 30% to 40% after irrigating for 5 days to increase the emergence rate of Setaria viridis; adopting a fixed and quantitative drip irrigation and topdressing to achieve water-saving and efficient irrigation and nutrient supplementation; and timely mowing and harvesting according to the growth characteristics of the plant to increase the biological yield and seed quality of Setaria viridis. The method improves the production efficiency of Setaria viridis, accelerates the grassland establishment process of Setaria viridis, and provides technical support for ensuring the promotion and utilization of Setaria viridis in the arid areas of Northwest China.

The invention relates to a traditional Chinese medicine composition for treating nutritional state decline after chemotherapy and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 9-40 parts of codonopsis pilosula, 6-30 parts of fried rhizoma atractylodis macrocephalae, 6-30 parts of poria cocos, 6-20 parts of processed rhizoma pinelliae, 6-12 parts of pericarpium citri reticulatae, 6-40 parts of fried hawthorn, 6-40 parts of fried medicated leaven, 6-40 parts of fried grain malt, 6-40 parts of caulis spatholobi, 6-40 parts of pyrrosia lingua, 6-40 parts of hairyvein agrimony, 3-15 parts of honey-fried licorice root and 6-30 parts of Chinese date. Compared with the prior art, the traditional Chinese medicine composition disclosed by the invention not only can relieve gastrointestinal symptoms during chemotherapy and effectively improve delayed nausea and vomiting, but also can inhibit weight loss caused by chemotherapy, improve myelosuppression after chemotherapy and the nutritional state of a patient and improve the living quality of the patient.

The invention belongs to the technical field of millet nematode prevention and control, and particularly relates to a millet nematode prevention and control method based on a grain seed period, which comprises the following steps: S1, putting millet seeds into a nematicide; s2, then the seeds are taken out and covered; s3, putting the stuffed seeds into a gelatinous coating agent, uniformly stirring, and then scattering the seeds together with the coating agent into the seeding furrows; wherein the nematicide is prepared from fluopyram with the concentration of 0.15 to 0.3 percent, millet plant ash with the concentration of 1 percent and brassinolide with the concentration of 3 percent. The nematicide formed by compounding the fluopyram, the millet plant ash and the brassinolide is used for closing the millet seeds, and the nematode control rate reaches 90% or above.

The present invention provides a method for detecting the copy number of exogenous gene insertion in Setaria viridis or foxtail millet by digital PCR, belonging to the field of biotechnology. The method uses the Sevir.3G057200 gene as an internal reference gene and Hyg the selectable marker gene as the exogenous gene, and uses the primer pairs 200-F / 200-R and Hyg-F / Hyg-R to detect the genomic DNA of transgenic Setaria viridis or transgenic foxtail millet, and to determine the copy number of exogenous gene insertion in transgenic Setaria viridis or transgenic foxtail millet. The method of the present invention has the characteristics of being simple, rapid, low sample consumption, efficient, and high-throughput, can improve the efficiency of screening transgenic lines of Setaria viridis or foxtail millet with low-copy insertion, and provides a new option for the detection method of the copy number of exogenous gene insertion in the research of transgenic Setaria viridis or transgenic foxtail millet.

The invention discloses diphosphonic acid and a salt compound thereof, a preparation method and application. The structural formula of the bisphosphonic acid and the salt compound thereof is shown as a formula (I). According to the preparation method, methyl (3-oxopropyl) phosphinic acid is taken as an initial raw material, a target product [1-hydroxy-3-(methylphosphonyl) propyl]-methylphosphinic acid is prepared through condensation and hydrolysis two-step reaction, and the target product reacts with an alkaline substance to obtain corresponding salt. The obtained target product has a bisphosphonic acid skeleton, and the bisphosphonic acid herbicide lead compound with a novel structure is beneficial to improving the weed removal effect; meanwhile, the target product is salified to obtain a salt compound, the salt compound is used for preparing herbicides for preventing and controlling green bristlegrass, amaranthus retroflexus, chenopodium album and other weeds, the obvious weeding effect is achieved, the water solubility, hygroscopicity, stability and the like of the compound can be changed by preparing the salt compound into the salt, and therefore different preparation requirements are met.

The application discloses a kind of bisphosphonic acid and its salt compound, preparation method and application.The structural formula of the bisphosphonic acid and its salt compound is as shown in formula (I).The preparation method is to prepare target product [1-hydroxy-3-(methyl phosphine acyl) propyl]-methyl hypophosphorous acid by condensation and hydrolysis two-step reaction with methyl (3-oxo propyl) hypophosphorous acid as starting material, and the target product is reacted with basic substance to obtain corresponding salt.The target product obtained by the application has bisphosphonic acid skeleton, and the novel bisphosphonic acid herbicide lead compound is beneficial to improve weed removal effect;Meanwhile, the salt compound obtained by salifying the target product is used to prepare herbicide for preventing and treating setaria viridis, amaranthus retroflexus, small gooseweed and other weeds, and has obvious weeding effect, and preparing salt can improve water solubility, hygroscopicity, stability and the like of the compound, so as to adapt to different formulation requirements.