13results about How to "Clear band" patented technology

The invention belongs to the technical field of biomolecules, and particularly relates to an NIPBL gene for judging the sex of chickens and application of the NIPBL gene. The NIPBL gene has a segment with the difference of 606bp on chicken Z and W chromosomes, and is also a deletion segment on the W chromosome, the chicken sex can be quickly and effectively identified by detecting the sequence condition, the chicken with the deletion of the molecular marker sequence segment is a female individual, and the chicken without the deletion of the molecular marker sequence segment is a male individual; the invention further provides a specific primer, the chicken sex can be accurately judged through a PCR amplification technology and agarose gel electrophoresis, a strip is clear, the method is small in technical difficulty, easy and convenient to operate and high in accuracy, the sex of chicks or chickens difficult to distinguish in appearance can be effectively identified, the method has practical significance on identification of varieties small in male and female appearance difference, and the method is worthy of popularization and application. The method disclosed by the invention is strong in technical applicability and good in repeatability, can be used for accurately identifying the gender of the poultry, and has a wide application prospect.

The invention provides a primer group for identifying astragalus mongholicus and an identification method of astragalus mongholicus, and belongs to the technical field of molecular markers, the primer group comprises a primer 186781F and a primer 186781R; the nucleotide sequence of the 186781F is as shown in SEQ ID NO. 1, and the nucleotide sequence of the 186781R is as shown in SEQ ID NO. 2. The primer group provided by the invention is designed aiming at the SSR molecular marker, and the SSR molecular marker has the advantages of uniform distribution, rich polymorphism, good amplification repeatability, clear banding pattern and the like in an astragalus membranaceus genome, and is suitable for detection of a common denaturing polyacrylamide gel electrophoresis detection platform and a capillary fluorescence detection platform; the method can be further applied to the research fields of astragalus genetic diversity analysis, variety identification, DNA fingerprint construction, molecular marker-assisted breeding and the like.

This invention belongs to the field of hybrid identification technology of *Fraxinus pubescens*, specifically relating to an InDel marker and its application for identifying the authenticity of hybrids between *Fraxinus pubescens* and *Astragalus membranaceus*. The InDel marker described in this invention is located at positions 187 to 240 of the sequence shown in SEQ ID NO:1, and the sequence of the InDel marker is shown in SEQ ID NO:2. Furthermore, based on this InDel marker, this invention has obtained a set of primers with stable polymorphism, high amplification efficiency, and clear bands, establishing a technical system that can identify the authenticity of hybrid offspring at the seedling stage without relying on expensive equipment. This provides an efficient and low-cost molecular tool for the breeding of new varieties among *Fraxinus pubescens* species, the protection of variety rights, and market supervision, and has significant application value.

The invention belongs to the technical field of biomolecules, and particularly relates to an SPIN1 gene for judging the sex of chickens and application of the SPIN1 gene. The SPIN1 gene has a segment of segment with the difference of 330bp on chicken Z and W chromosomes, and the segment is a deletion segment on the Z chromosome, by detecting the sequence condition, the chicken sex can be quickly and effectively identified, the chicken with the deletion of the molecular marker sequence segment is a female individual, and the chicken without the deletion of the molecular marker sequence segment is a male individual; the invention further provides a specific primer, the chicken sex can be accurately judged through a PCR amplification technology and agarose gel electrophoresis, a strip is clear, the method is small in technical difficulty, easy and convenient to operate and high in accuracy, the sex of chicks or chickens difficult to distinguish in appearance can be effectively identified, the method has practical significance on identification of varieties small in male and female appearance difference, and the method is worthy of popularization and application. The method disclosed by the invention is strong in technical applicability and good in repeatability, can be used for accurately identifying the gender of the poultry, and has a wide application prospect.

The invention belongs to the technical field of molecular marker technology development and application, and discloses an SSR molecular marker developed based on a mactra veneriformis reference transcriptome sequence, and the SSR molecular marker comprises 16 nucleotide sequences corresponding to microsatellite markers as shown in SEQ ID NO.1-SEQ ID NO.16 in sequence. At present, no report about development of mactra veneriformis microsatellites from genomes and transcriptomes exists, 16 stable microsatellite molecular markers with clear strips and high polymorphism are developed from mactra veneriformis transcriptome data on the basis of a high-throughput transcriptome sequencing technology for the first time, and the microsatellite molecular markers can be used for identifying the mactra veneriformis microsatellites. The method has good practical production and application values in the fields of mactra veneriformis population genetics analysis, genetic relationship identification, high-density genetic map construction and molecular marker-assisted breeding.

This invention relates to the field of agricultural technology, specifically providing a specific primer pair for identifying hybrid offspring of wild rice, cultivated rice, and cultivated rice and wild rice, and its application. Genomic DNA is extracted from rice leaves. Using this DNA as a template and the sequences corresponding to SEQ ID NO:3 and SEQ ID NO:4 as specific primers, PCR amplification is performed to obtain amplified fragments. After electrophoresis, fragments showing only a 146 bp major band are identified as wild rice, while those showing only a 155 bp major band are identified as cultivated rice. Fragments that simultaneously amplify both 155 bp and 146 bp fragments are identified as hybrid offspring of cultivated rice and wild rice. This invention can be applied to molecular marker detection and polymorphism detection in hybrid populations of wild rice and cultivated rice, facilitating rapid screening in distant hybridization breeding and improving breeding efficiency.

The present invention discloses a method for monitoring the germplasm of chuatsi (Siniperca chuatsi) using microsatellite genetic markers, including extraction and detection of genomic DNA, search and analysis of microsatellite sequences, screening and optimization of polymorphic microsatellite primers, detection of polymorphic microsatellites, and monitoring of different geographical chuatsi populations and their genetic traits; the present invention screens a large number of ESTs generated by chuatsi transcriptome sequencing for microsatellite sites and develops and verifies polymorphic microsatellite markers, and develops 15 EST-SSR polymorphic microsatellite markers, which can monitor different geographical populations of chuatsi and their genetic traits. The method can make up for the limitations of the current chuatsi germplasm detection, improved variety breeding and germplasm improvement research, such as the relatively vague genetic background and the small number of molecular markers, and provide a basis for the chuatsi germplasm detection, improved variety breeding and germplasm improvement, thereby promoting the progress of chuatsi molecular marker germplasm detection, assisted genetic breeding and genomics research.

The invention discloses a molecular marker of a rice cold-resistant gene and application thereof, and relates to the field of rice breeding. The molecular marker is located in a promoter region (-307-364) of a rice seedling stage cold-resistant gene LTSS9a (LOCOs09g21710). The invention provides a primer for detecting the molecular marker of the rice seedling stage cold-resistant gene and a kit comprising the primer. The invention also provides application of the molecular marker in identifying the cold resistance of the rice in the seedling stage and a method for identifying the cold resistance of the rice in the seedling stage. The molecular marker provided by the invention has high accuracy and genetic stability, is stable in performance under different generations and environmental conditions, and is not easy to mutate; the method is simple and convenient to operate and visual in result, and can be used for rapidly identifying the rice seedling-stage cold-resistant variety, so that a powerful tool is provided for research on rice cold resistance, and the problem of rice yield reduction caused by low-temperature cold damage is effectively solved.

The present invention relates to the technical field of pathogen molecular detection, and specifically to a primer, a kit and a method for detecting Staphylococcus aureus. The sequences of the primers are shown in SEQ ID NO.1 and SEQ ID NO.2. The present invention also discloses a kit comprising the primers, and a method for detecting Staphylococcus aureus using the primers. The detection limit of the method for detecting Staphylococcus aureus is 3.06 copies / μL, which is higher than similar studies, equivalent to 8.4 times that of the LAMP method, 12.5 times that of the fluorescent quantitative PCR method, and 125 times that of the conventional PCR method, and does not rely on complex variable temperature instruments, so it is suitable for grassroots front-line detection institutions and can provide a feasible technical means for the rapid detection of Staphylococcus aureus.

The present application relates to a kind of SSR molecular marker primer set of Mahonia, and its application, the forward primer sequence of the SSR molecular marker primer set includes the sequence as shown in any one of SEQ ID NO:1-7 or as shown in any one of SEQ ID NO:1-7;The reverse primer sequence of the SSR molecular marker primer set includes the sequence as shown in any one of SEQ ID NO:8-14 or as shown in any one of SEQ ID NO:8-14, the SSR molecular marker primer set of the present application is accurately identified long column Mahonia, and it guarantees the authenticity of base original variety for its relevant quality standard research.

The application belongs to the technical field of identification of Fraxinus velutina hybrid, and particularly relates to an InDel marker for identifying the authenticity of Fraxinus velutina hybrid and application thereof. The InDel marker is located at positions 63-150 of the sequence shown in SEQ ID NO:2, and the sequence of the InDel marker is shown in SEQ ID NO:1. Meanwhile, based on the InDel marker, the application obtains a group of primers with stable polymorphism, high amplification efficiency and clear bands, and establishes a technical system for identifying the authenticity of hybrid offspring at the seedling stage without relying on expensive equipment, thereby providing an efficient and low-cost molecular tool for Fraxinus velutina new variety breeding, variety right protection and market supervision, and having good application value.

The present application relates to gradient gel and its preparation method and application, the gradient gel includes separation gel of different concentrations, and the separation gel includes components of the following concentrations: 0.02wt%-0.1wt% N,N,N',N'-tetrakis(2-hydroxyethyl)ethylenediamine, 0.1wt%-0.3wt% sodium bisulfite, 0.1wt%-0.2wt% ammonium persulfate and 1wt%-15wt% trehalose. The gradient gel has the advantages of high resolution, clear bands, good separation effect and low toxicity. The formula of the present application is more suitable for the preparation of gradient gel, and the combination of components in the formula makes it more suitable for automated glue filling equipment to realize automated production of gradient gel.

The invention provides a material for real-time detection of stem cell differentiation, 2DMXene is taken as a matrix material, the matrix material of 2DMXene is Nb2CTx, chiral amino acid, nanogold and methylene blue are loaded on the matrix material of 2DMXene, the matrix material is recorded as chiral RH6G-Au / Nb2CTx, RH6G represents methylene blue, Au represents nanogold, chirality represents that Au / Nb2CTx reacts with the chiral amino acid to form a chiral material, the matrix material of 2DMXene is Nb2CTx, and the matrix material of 2DMXene is Nb2CTx and is recorded as chiral RH6G-Au / Nb2CTx is recorded as chiral RH6G-Au / Nb2CTx. The thickness of the material for real-time detection of stem cell differentiation is 2.5-3.5 nm, and the ratio of the longest diameter to the shortest diameter of a two-dimensional surface, perpendicular to the thickness direction, of the material for real-time detection of stem cell differentiation is 1.0-1.5; in the material for real-time detection of stem cell differentiation, the weight ratio of the nanogold material to the Nb2CTx material is x: (100-x), and x is more than or equal to 10 and less than or equal to 30. The material provided by the invention has high resolution on the functional neuron marker dopamine, clear strip and higher sensitivity, and realizes high-sensitivity and high-selectivity detection on the functional neuron marker dopamine differentiated by stem cells.