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Method for identifying DNA barcodes of three gentiana macrophylla medicinal materials in pharmacopeia

A technology of barcode and Gentiana, applied in the field of DNA barcode identification

Active Publication Date: 2014-01-15
ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the identification of combined fragments for Gentiana chinensis has not been reported. This study explored the barcode combined fragments suitable for Gentiana chinensis.

Method used

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  • Method for identifying DNA barcodes of three gentiana macrophylla medicinal materials in pharmacopeia
  • Method for identifying DNA barcodes of three gentiana macrophylla medicinal materials in pharmacopeia
  • Method for identifying DNA barcodes of three gentiana macrophylla medicinal materials in pharmacopeia

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Experimental program
Comparison scheme
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Embodiment Construction

[0020] 1 Experimental equipment and reagents

[0021] 1.1 Experimental Instruments

[0022] DDY-6C electrophoresis instrument (Beijing Liuyi Instrument Factory), T196 PCR amplification instrument (German Biometra), GeCDOCXR gel imaging system (Bio-RAD, USA), HANGPINGJA3003N electronic analytical balance, KM-15200KUBOTA centrifuge, eppendorfCentrrfuge5417R ultra-speed freezing Centrifuge (Eppendorf, Germany), Guohua HH-60 digital display constant temperature stirring circulating water tank, BCD-2181E refrigerator (Royalstar), Panasonic NM-S553MF microwave oven (Shanghai Panasonic Microwave Oven Co., Ltd.), KQ-250DB CNC ultrasonic cleaner ( Kunshan Ultrasonic Instrument Co., Ltd.), SAXXYO MDF-382E-80℃ ultra-low temperature refrigerator (Japan), Aquapro AYJ1-0501-U ultrapure water system (Yiyang Enterprise Development Co., Ltd.), LQP-B-4 ice making Machine (Liuquan Anke), PHOTOA drying oven (Shanghai Yiheng Technology Co., Ltd.), SK-1 fast mixer (Guohua), TopPette manual single-...

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Abstract

The invention discloses a method for identifying DNA (Deoxyribose Nucleic Acid) barcodes of three gentiana macrophylla medicinal materials in the pharmacopeia. Three gentian macrophylla medicinal materials in the pharmacopeia are respectively Gentiana macrophylla Pall, G.crassicaulis Duthie, and G.straminea Maxim. The identification method comprises two steps of carrying out DNA extraction and splicing obtained DNA sequences, wherein in the DNA extraction step, a good extraction effect is obtained by prolonging cracking heating time and utilizing phenol and trichloromethane, between which the volume ratio is 1:1, to remove interference of phenol and polysaccharide substances; then the obtained DNA sequences are spliced to identify gentiana macrophylla so as to obtain a good identifying effect.

Description

technical field [0001] The invention relates to a DNA barcode identification method of three Gentiana chinensis medicinal materials in the Pharmacopoeia. Background technique [0002] Gentiana chinensis is a traditional Chinese medicine commonly used in traditional Chinese medicine. It was first recorded in "Shen Nong's Materia Medica", and it was listed as a "medium grade". Traditional Chinese medicine believes that it has the effects of dispelling rheumatism, clearing damp-heat, relieving numbness and pain, and reducing asthenic heat. With the rapid development of modern medical science and technology in the research of chemical components, pharmacological effects, and clinical applications, the pharmacological effects of Gentiana chinensis have been further discovered, and clinical applications have become more and more extensive. Pharmacological research and clinical application have shown that Gentiana chinensis not only has obvious effects on anti-inflammatory and ana...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2563/185
Inventor 方成武张明燕孟楣彭华胜马宗华黄璐琦刘守金杨青山李庆林
Owner ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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