Method for identifying soybean cytoplasmic male sterile line seed purity through molecular marker

Abstract

The invention provides a method for identifying soybean RN type cytoplasmic male sterile line seed purity through a molecular marker. The method comprises the steps of: extracting genomic DNA of an RN type cytoplasmic male sterile line seed; carrying out PCR (Polymerase Chain Reaction) amplification by taking the genomic DNA as a template and utilizing a primer InDel-cms1, and carrying out gel electrophoresis analysis on an amplification product, wherein the length of a sterile line fragment after amplification is 200bp, and the length of a maintainer line fragment after amplification is 212bp; and accurately distinguishing the sterile line and the maintainer line through the length difference of the fragments, wherein the seed with the amplified fragment different from the amplified sterile line fragment is a false sterile line seed, and the percentage of the number of sterile line fragments with specific length obtained by detection accounting for all the detected seeds is the purity of RN type cytoplasmic male sterile line seeds. The method can be used for rapidly, accurately and reliably identifying the maintainer line mixed in the RN type soybean cytoplasmic male sterile line by a molecular biology experiment means so as to ensure the purity of sterile line seeds.

Description

Technical field: [0001] The invention relates to the technical field of molecular marker detection, and relates to a method for molecular marker identification of soybean cytoplasmic male sterile line seed purity, which is suitable for identification of soybean RN type cytoplasmic male sterile line seed purity. Background technique: [0002] The "three lines" of soybean cytoplasmic male sterility consist of a sterile line, a maintainer line and a restorer line. Hybrids can be obtained by crossing the female parent of the sterile line with the male parent of the restorer line. Among them, the maintainer line and the restorer line are self-fertile, and seeds can be obtained by self-propagation; the sterile line itself is sterile, and seeds can only be obtained by crossing it as a female parent with the male parent of the same-type maintainer line. Except for fertility, the maintainer lines have the same agronomic traits. In the process of reproduction, the results of mixed ...

AI Technical Summary

Problems solved by technology

The hybrids thus harvested are impure due to the inclusion of sterile lines

The planting of such seeds in production will inevitably lead to the emergence of sterile plants in the production field and reduce production, which will reduce the enthusiasm of farmers for planting, and the seed sales company will also bear a large risk of default

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