Polymorphism micro-satellite DNA molecular marker for deer and application of polymorphism micro-satellite DNA molecular marker
A DNA molecule and microsatellite technology, applied in recombinant DNA technology, DNA/RNA fragment, microbial determination/inspection, etc. high polymorphism, clear bands, and rich genetic information
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[0038] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market.
[0039] 1. Genome extraction and detection of sika deer.
[0040] Take 300ul of sika deer blood, use the Biotake blood extraction kit to extract the sika deer genome, use 1% agarose gel electrophoresis to detect the integrity of the DNA, measure the DNA concentration and purity with a microplate reader, and store it at -20°C for later use.
[0041] 2. Si...
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