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Polymorphism micro-satellite DNA molecular marker for deer and application of polymorphism micro-satellite DNA molecular marker

A DNA molecule and microsatellite technology, applied in recombinant DNA technology, DNA/RNA fragment, microbial determination/inspection, etc. high polymorphism, clear bands, and rich genetic information

Active Publication Date: 2017-09-15
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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Problems solved by technology

At present, in the molecular breeding research on sika deer, most of the polymorphic microsatellite molecular markers used are based on the published bovine microsatellite primers, only a small part is obtained through traditional cloning methods, and these microsatellites The polymorphism of the locus is not high, which cannot meet the requirements of protection, research, management and molecular breeding of sika deer

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  • Polymorphism micro-satellite DNA molecular marker for deer and application of polymorphism micro-satellite DNA molecular marker
  • Polymorphism micro-satellite DNA molecular marker for deer and application of polymorphism micro-satellite DNA molecular marker
  • Polymorphism micro-satellite DNA molecular marker for deer and application of polymorphism micro-satellite DNA molecular marker

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Embodiment Construction

[0038] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market.

[0039] 1. Genome extraction and detection of sika deer.

[0040] Take 300ul of sika deer blood, use the Biotake blood extraction kit to extract the sika deer genome, use 1% agarose gel electrophoresis to detect the integrity of the DNA, measure the DNA concentration and purity with a microplate reader, and store it at -20°C for later use.

[0041] 2. Si...

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Abstract

The invention relates to a micro-satellite DNA molecular marker for cervine animals. The molecular markers can be used for identifying allelic polymorphism, identify same or related cervine animals, distinguish the cervine animals and research the genetic diversity of the species group. The molecular marker can also be used for the genetic and phenotype research utilizing a statistics method such as linkage analysis, association mapping, linkage imbalance. The information can be used for breeding and / or selecting plants.

Description

technical field [0001] The invention relates to the field of molecular genotyping, in particular to polymorphic microsatellite DNA molecular markers for deer and uses thereof. Background technique [0002] The sika deer (Cervus nippon) is mainly distributed in East Asia, ranging from Siberia to Korea, eastern China and Vietnam; it is also distributed in Japan and other western Pacific islands. Chinese sika deer are mainly distributed in Jilin Province, while Japanese sika deer are mainly distributed in Hokkaido. In the 19th century, the sika deer was hunted to almost extinction. Legislation and protection began in the middle of the 20th century, and the population recovered rapidly from the 1950s to the 1980s. Sika deer have also been introduced to Australia, Europe and the United States. Originally intended as park animals, many are now wild. At present, the subspecies of sika deer distributed in China and Japan have been listed as critically endangered or endangered by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 王桂武杨万云
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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