Method for screening pollination pear varieties suitable for Dangshan pears

Abstract

A method for screening pollination pear varieties suitable for Dangshan pears comprises the specific steps that 1, DNA of leaves of the pollination pear varieties is extracted; 2, a SCoT-PCR is carried out, a genetic similarity coefficient is calculated, and a genetic relationship dendrogram between the pollination pear varieties is constructed; 3, according to a clustering analysis result, the pollination pear varieties with the far, intermediate and near genetic relationships are selected to pollinate the Dangshan pears; 4, the number and area of sclereid groups of pulp tissue of mature fruits borne by each pollination pear variety are microscopically observed and calculated, and the content of sclereid is measured; 5, the pollination pear varieties with the optimal generic relationship are screened out through the analysis result of the step 4. According to the method, the genetic relationships between different pear variety resources can be revealed fast and accurately from the molecular level, the influence of the different genetic relation pollination pear varieties on the size and content of the sclereid of Dangshan pear fruits is analyzed, then the excellent pollination varieties with the proper genetic relationship are screened out, the sclereid content of the Dangshan pear fruits is improved, and the quality of the Dangshan pear fruits is improved.

Description

technical field [0001] The present invention relates to the field of pollination biology of horticultural crops / fruit tree production technology, and in particular to a method of using SCoT molecular markers to determine the genetic relationship between a male parent tree and Dangshansu pear, and to regulate the pollination of Dangshansu pear fruit by selecting pollinating trees with different genetic relationships The area and content of stone cell clusters were used to finally screen out the method of Dangshansu pear suitable for pollination trees. Background technique [0002] Dangshansu pear is one of the main pear export varieties in my country. It has a large planting area and high yield. Because its self-pollination fruit setting rate is extremely low, it must be equipped with suitable pollination trees in production. However, due to improper allocation of pollinated varieties and unscientific field management, the quality of Dangshansu pears has gradually declined. T...

AI Technical Summary

Problems solved by technology

It can be seen that the selection of pollination trees has an important impact on the quality of pear fruit. However, fruit farmers in most areas choose local fixed pollination trees based on production experience, which often reduces the overall yield and economic benefits of the orchard due to improper allocation. , against planting enthusiasm

[0004] Start codon targeted polymorphism (SCoT) marking is a new DNA molecular marking technology that has emerged in recent years. It has the characteristics of high polymorphism, good repeatability, and strong primer versatility. The diversity of plant germplasm resources has been widely used, but there are few reports on the diversity and genetic relationship of pear germplasm resources

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