Molecular marker of wheat scab resistance expansion gene Fhbl and application thereof

A technology of molecular markers and extended genes, which is applied in the determination/testing of microorganisms, DNA preparation, DNA/RNA fragments, etc., can solve the problem of poor agronomic traits of scab-resistant germplasm, time-consuming and labor-intensive resistant varieties, and susceptibility to environmental influences and other problems, to achieve the effect of improving the efficiency of selection and identification, convenient detection, and convenient identification

Inactive Publication Date: 2012-09-05
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] Because wheat head blight resistance has typical quantitative traits and is easily affected by the environment, it is difficult to select for it in early generations
In addition, many scab-resistant germplasms have poor agronomic traits and strong regional adaptability. It is time-consuming and labor-intensive to breed resistant varieties using traditional breeding methods and may not yield the expected results

Method used

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  • Molecular marker of wheat scab resistance expansion gene Fhbl and application thereof
  • Molecular marker of wheat scab resistance expansion gene Fhbl and application thereof
  • Molecular marker of wheat scab resistance expansion gene Fhbl and application thereof

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Experimental program
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Effect test

Embodiment 1

[0039] The above-mentioned molecular marker of the wheat head blight resistance extended gene Fhb1 was obtained by the following method:

[0040] (1) Wangshuibai Fhb1 near-isogenic line NMAS016 and its recurrent parent Mianyang 99-323F 2:3 Creation of populations and screening of Fhb1 segment recombinants:

[0041] (1) NMAS016(♀) and wheat variety Mianyang 99-323 hybrid F 1 , F 1 Selfing produced F containing 2875 individuals 2 group;

[0042] (2) Using the boundary markers Xbarc147 and Xgwm493 of Fhb1 (http: / / wheat.pw.usda.gov / GG2 / ) in Fhb1 2 In the population, 71 heterozygous individuals with recombination in this segment were screened, and the same marker was used in its F 3 99 homozygous individuals recombined in this segment were obtained through screening.

[0043] (2) Identification of recombinant disease-resistant phenotypes

[0044] The recombinants were inoculated by single flower dripping method when they bloomed. 15 days after inoculation, the number of d...

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Abstract

The invention belongs to the field of crop breeding, and relates to a molecular marker of wheat scab resistance expansion gene Fhbl and application thereof. The molecular marker is one of MAG6067 and MAG6660 with a genetic distance to an Fhbl gene being respectively 0.05cM and 0cM. A molecular marker which is closest linked to the Fhbl for the first time internationally by utilizing the invention. The Fhbl gene can be detected by marking the MAG6660 to determine whether the Fhbl exists or not and the existing state of the Fhbl, and the scab resistance of wheat can be predicted, so that a plant carrying the Fhbl can be quickly screened; and the molecular marker can be used for breeding a scab-resistance variety. Furthermore, laboratory detection can be carried out by utilizing the molecular marker to avoid the influence of the environment to the phenotype. According to the molecular marker closely linked with the Fhbl, time and labor for screening scab resistance materials can be greatly saved, the prediction accuracy can be enhanced, and clone of the Fhbl gene can be accelerated.

Description

technical field [0001] The invention belongs to the field of crop breeding, and relates to a molecular marker of wheat scab-resistant extended gene Fhb1 and its application. technical background [0002] Wheat is one of the most important food crops in the world, providing about 20% of human energy. Many biotic and abiotic stresses directly affect the yield and quality of wheat in wheat production. Scab is one of the most important biological adversities, which seriously affects the quality and yield of wheat. The breeding and application of disease-resistant varieties is the most economical and effective method to control scab. The discovery of disease-resistant genes is the premise and foundation of disease-resistant breeding, and the development of molecular markers closely linked to disease-resistant genes is the most important way to apply disease-resistant genes. key. [0003] There are five main types of wheat scab resistance (Mesterhazy 1995), of which resistance ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 马正强薛树林李国强郜忠霞
Owner NANJING AGRICULTURAL UNIVERSITY
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