39results about How to "Improve selection identification efficiency" patented technology

The invention belongs to the technical field of molecular breeding, and provides an SNP molecular marker linked with a wheat stripe rust resistance gene QYr.sicau-1B.1 and application thereof. The SNPmolecular marker is KASP-Yr, which can be obtained by amplifying a primer having a nucleotide sequence as shown in SEQ ID NO.1-3. Detection analysis indicates that the molecular marker can be used for accurately tracking wheat stripe rust resistant QTL QYr.sicau-1B.1 and predicting the stripe rust resistance characteristics of wheat, so that molecular designed breeding can be performance conveniently. The invention further discloses a method for identifying the molecular marker linked with wheat stripe rust resistance QTL QYr.sicau-1B.1. By utilizing the method, the stripe rust resistance prediction accuracy can be reinforced to quickly screen wheat variety or strain with stripe rust resistance for breeding, the breeding process for high-yield variety of wheat can be greatly accelerated.

The invention discloses a molecular marker for efficient utilization of a QTL site by barley phosphorus and an application of the marker. The molecular marker is Hvc630, a nucleotide sequence of the marker is shown in SEQ ID NO: 1 and is positioned on a barley 3H chromosome, and thus the marker can accurately track the QTL site efficiently utilized by the barley phosphorus, predict barley phosphorus utilization efficiency characteristics, and further facilitate molecular design breeding for nutrient efficient utilization. The invention further provides the application of the molecular marker Hvc630 in barley breeding with high-yield high-efficiency nutrients. By using the method, the accuracy of barley phosphorus utilization efficiency prediction can be enhanced, and barley varieties or strains with QTL for improving barley phosphorus utilization efficiency can be rapidly screened for breeding, so that the breeding process of high-yield high-efficiency barley varieties can be greatly accelerated.

The invention relates to the field of barley molecular breeding, and in particular discloses a molecular marker HRM1 of barley grain length gene LkI1 and application of the molecular marker. The nucleotide sequence of the molecular marker HRM1, which is closely linked with the barley grain length gene LkI1, is as shown in SEQ ID No.1. Upon detection and analysis, the molecular marker can accurately track the barley grain length gene and predict the grain length traits of the barley, so as to facilitate molecular designing and breeding. The invention also discloses a method for identifying the molecular marker of the barley grain length gene LkI1; by virtue of the method provided by the invention, the accuracy of grain length prediction is enhanced, so that barley varieties or lines having enhanced grain length QTL can be conveniently and rapidly screened out; and the process of selecting high-yield barley varieties can be greatly accelerated.

The invention relates to the field of genetic breeding, and particularly relates to SNP molecular markers linked with rice sodium and potassium ion absorption QTL qK/Na-3-1, primers and application. The SNP molecular markers RS (36172492) and RS (36174628) are located on a Chr3 chromosome short arm of an IRGSP-1.0 genome version, the sequences are shown as SEQ ID NO. 1 and SEQ ID NO. 2, the polymorphisms are (T/G) (G/-), (A/-), and the SNP molecular markers can be obtained by amplifying the primers shown as SEQ ID NO. 3 and SEQ ID NO. 5. The molecular markers can accurately track the rice sodium and potassium ion absorption QTL qK/Na-3-1 and predict the potential salt tolerance characteristic of rice, and then molecular breeding is facilitated. The invention further discloses a method foridentifying the rice sodium and potassium ion absorption QTL qK/Na-3-1 molecular markers. By means of the method, the accuracy of sodium and potassium ion absorption prediction can be enhanced, so that rice varieties or strains with potential enhanced rice salt tolerance are quickly screened out for breeding, and the breeding process of the rice varieties with potential salt tolerance can be greatly accelerated.

The invention discloses a wheat maximum root length QTLQMrl.sau-3D linked SNP (Single Nucleotide Polymorphism) molecular marker and application thereof, and relates to the field of wheat molecular genetic breeding. The SNP molecular marker and QMrl.sau-3D are co-localized on a 3D chromosome long arm of a wheat genome, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.16, and C/T mutation exists at the 36bp position of the SNP molecular marker. The SNP molecular marker KASP-Mrl-sau1 is closely linked with the maximum root length QTLQMrl.sau-3D of wheat, the maximum root length QTLQMrl.sau-3D of wheat can be accurately tracked, the maximum root length characteristic of wheat can be predicted, the selection and identification efficiency of the maximum root length of wheat can be remarkably improved, wheat varieties or strains with the long root length can be rapidly screened out, and the application prospect is wide. The process of wheat molecular genetic breeding is accelerated.

The invention belongs to the field of crop breeding, and discloses molecular markers of a wheat scab infection resistance gene Fhb4 and application of the molecular markers. The molecular markers are selected from any one of MAG5184, MAG7237 and MAG2521, and the genetic distances to Fhb4 gene are 0.72cM, 0.34cM and 0.92cM, respectively. According to the invention, the molecular markers having the closest linkage with Fhb4 are obtained for the first time in the world. These three molecular markers are codominant and have the advantages of convenient detection, and stable, simple and convenient amplification. The molecular marker MAG7237 is used to detect Fhb4 gene to determine the existence and status of Fhb4 and predict the scab resistance of wheat, and then to screen the plants with Fhb4 gene, which are used for breeding disease-resistant varieties.

The invention discloses a KASP molecular marker linked to wheat flag leaf length QTL QFll-2B and application thereof. The molecular marker is located on the long arm of chromosome 2B of a RefSeqv1.0 genome. The detection and analysis show that the molecular marker can accurately track the wheat flag leaf length QTL QFll-2B, predict wheat flag leaf length characteristics, and then facilitate molecular breeding. The invention also discloses a method for identifying the molecular marker of the wheat flag leaf length QTL QFll-2B, and the method provided by the invention can be used to enhance theaccuracy of predicting the flag leaf length, and can screen wheat varieties or lines with longer flag leaves more quickly for wheat breeding, greatly accelerating the process of wheat breeding.

The invention belongs to the technical field of crop molecular genetic breeding, and discloses a KASP molecular marker linked with wheat stripe rust resistance QTL and application. The nucleotide sequence of the KASP molecular marker comprises a first nucleotide sequence and a second nucleotide sequence; and the first nucleotide sequence is SEQ ID NO: 1, and the second nucleotide sequence is SEQ ID NO: 2. The molecular marker linked with wheat stripe rust resistance QTL and located on a wheat 2A chromosome is a flanking marker of a stripe rust resistance site QYr.sicau-2AS on a short arm of the wheat 2A chromosome, and the linkage degree is high. The marker can be used for detecting the stripe rust resistance site QYr.sicau-2AS on the wheat 2A chromosome, plants with the loci are rapidly screened, and then the wheat disease resistance breeding efficiency is improved.