A molecular marker hrm5 of wheat oligo-tiller gene ltn3 and its application
A molecular marker, wheat technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problems of high cost, long time, and high difficulty in selection, and achieve high accuracy and success rate. High, improve the effect of selection and identification efficiency
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Embodiment 1
[0049] This embodiment is used to illustrate the acquisition method of wheat oligo-tiller gene Ltn3 and molecular marker HRM5:
[0050] (1) Using oligo-tiller wheat H461 as the female parent and wheat Chuannong 16 as the male parent to cross hybrids to obtain hybrid F1, F1 generation F2 was obtained by selfing a single plant, and the F8 generation RIL containing 223 lines was obtained by single seed propagation method 188 lines were randomly selected to form a genetic mapping population.
[0051] (2) Extract the DNA of each strain of the genetic mapping population with the CTAB method, and select the hexaploid wheat A, B, D published on wheatgenomics (http: / / wheatgenomics.plantpath.ksu.edu / ) The 90K SNP chip of the genome, using the DNA of the parents H461 and Chuannong 16 as templates, was used for genotyping to obtain the genotype data of the RIL population. The band pattern of the parent H461 is marked as A, and the band pattern of the parent Chuannong 16 is marked as B. ...
Embodiment 2
[0061] 1.1 Extraction of DNA
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