Molecular marker HRM1 of barley grain length gene LkI1 and application of molecular marker
A technology of molecular markers and barley grains, which is applied in the fields of application, microbial measurement/inspection, angiosperms/flowering plants, etc., to achieve the effect of improving selection and identification efficiency, high success rate, and high accuracy
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Embodiment 1
[0049] This embodiment is used to illustrate the method for obtaining the barley grain length gene Lkl1 and the molecular marker HRM1:
[0050] (1) Using long-grain long wild barley AWCS276 as the female parent and barley Baudin as the male parent to cross hybrids to obtain hybrid F1, F1 generation F2 was obtained by selfing a single plant, and the F8 generation RIL containing 130 lines was obtained by single-seed propagation method Populations constitute genetic mapping populations.
[0051] (2) Extract the DNA of each strain of the genetic mapping population with the CTAB method, use the DArT chip technology, and use the DNA of the parents AWCS276 and Baudin as a template to perform genotyping to obtain the genotype data of the RIL population . The band pattern of AWCS276 is marked as A, and the band pattern of parent Baudin is marked as B. The strain band type of F8 population derived from AWCS276 was marked as A, the strain derived from Baudin was marked as B, and the he...
Embodiment 2
[0061] 1.1 Extraction of DNA
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