SNP molecular markers linked with rice sodium and potassium ion absorption QTL and application thereof

A technology of molecular markers and ion absorption, applied in the field of molecular biology and crop genetics and breeding, can solve the problem of lack of molecular markers

Pending Publication Date: 2020-10-02
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Previously, some scholars conducted QTL mapping for sodium and potassium ion absorption, and found that related QTLs widely exist in rice. However, there are currently no closely linked molecular markers for ion channel protein genes related to rice sodium and potassium ion absorption.

Method used

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  • SNP molecular markers linked with rice sodium and potassium ion absorption QTL and application thereof
  • SNP molecular markers linked with rice sodium and potassium ion absorption QTL and application thereof
  • SNP molecular markers linked with rice sodium and potassium ion absorption QTL and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: Phenotypic identification of parental materials and rice F 1 Generation microspore DH population construction

[0059] (1) Based on the parents "Jiucaiqing" and "Shenyou-MG8", field hybridization, bagging, and harvesting hybrid F 1 generation seeds, grown in pots in the greenhouse, to obtain F 1 Generation plant, microspore culture, doubling, obtain microspore regenerated plant, doubling, selfing and fruiting, seed harvesting, obtain altogether 39 rice strains ( figure 1 ).

[0060](2) Using a hydroponic experiment, carry out salt stress treatment on the parents, adjust the salt concentration of the salt pond, measure the concentration of the salt pond every 2-3 days, and adjust it to 0.3%. After 30 days of salt stress, the second leaves of the plants in the salt pond were taken, and 3 plants were taken for each material. like figure 2 As shown, under salt stress, the K of variety SY-MG8 + =10.7±0.56(g / kg), Na + =3.24±0.15, K of JCQ + =10.42±0.08, Na...

Embodiment 2

[0061] Example 2: Phenotypic identification of rice DH progeny sodium and potassium ion absorption

[0062] K of DH population under salt stress treatment + / Na + Ion ratio was investigated, and the K under salt treatment was screened out + / Na + The 10 lines with the highest and lowest ion ratios. like image 3 As shown, the DH population K + / Na + The ratio distribution ranged from 0.62 to 9.18. In the two extreme mixed pools derived from the DH population, divided into high K + / Na + Than groups (H-bulks) and lower K + / Na + Compared with the population (L-bulks), the difference between the two extreme pools was significant (P=2.52×10 -8 ). The two populations of H-bulks and L-bulks each included 10 DH lines, and each DH line contained 6 individuals. DH strain K in L-bulks + / Na + The value is between 0.62 and 2.26, the DH strain K in L-bulks + / Na + The value is between 4.64 and 9.18, and the remaining 19 DH strains K + / Na + The value is between 2.31 an...

Embodiment 3

[0063] Example 3: Comparative analysis of the quality of sequencing data

[0064] (1) DNA was extracted from each strain, and sampling was repeated three times for each strain. Use the NanoDrop 2000 (cThermoScientific) instrument to measure the DNA concentration, and mix in equal amounts for the construction of high K + / Na + Than mixed pool (H-bulks) and lower K + / Na + Compare pools (L-bulks) for library preparation and detection.

[0065] (2) Library construction and sequencing: Use ultrasonic waves to fragment the parental and pooled DNA sequences to form random fragments, perform end repair on the fragmented DNA in sequence, add A to the 3' end, connect the sequencing adapter, and then use magnetic beads to absorb and enrich Fragments with a genome length of about 400 bp were amplified by PCR to form a sequencing library. The library that meets the quality inspection standard is sequenced by Illumina HiSeq, the average sequencing depth of the parents is 28×, and the ...

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Abstract

The invention relates to the field of genetic breeding, and particularly relates to SNP molecular markers linked with rice sodium and potassium ion absorption QTL qK/Na-3-1, primers and application. The SNP molecular markers RS (36172492) and RS (36174628) are located on a Chr3 chromosome short arm of an IRGSP-1.0 genome version, the sequences are shown as SEQ ID NO. 1 and SEQ ID NO. 2, the polymorphisms are (T/G) (G/-), (A/-), and the SNP molecular markers can be obtained by amplifying the primers shown as SEQ ID NO. 3 and SEQ ID NO. 5. The molecular markers can accurately track the rice sodium and potassium ion absorption QTL qK/Na-3-1 and predict the potential salt tolerance characteristic of rice, and then molecular breeding is facilitated. The invention further discloses a method foridentifying the rice sodium and potassium ion absorption QTL qK/Na-3-1 molecular markers. By means of the method, the accuracy of sodium and potassium ion absorption prediction can be enhanced, so that rice varieties or strains with potential enhanced rice salt tolerance are quickly screened out for breeding, and the breeding process of the rice varieties with potential salt tolerance can be greatly accelerated.

Description

technical field [0001] The invention relates to the fields of molecular biology and crop genetic breeding, in particular to a SNP molecular marker linked to rice sodium and potassium ion absorption QTLqK / Na-3-1 and its application. Background technique [0002] Rice is one of the most important food crops in the world, and it is also an important food crop in my country. The food situation in China is still severe. Improving the salt tolerance of rice can expand the planting area of ​​rice. my country has a vast coastal area and super-rich Saline-alkali land to be developed. Breeding suitable germplasm resources of salt-tolerant rice is of great significance to ensure our food security. [0003] Salt tolerance traits in rice are complex quantitative traits, controlled by multiple quantitative trait loci (QTL), and have the characteristics of low heritability, strong environmental influence, and high selection difficulty. Therefore, in the breeding process, traditional The t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 周龙华刘成洪郭桂梅何婷王亦菲陆瑞菊
Owner SHANGHAI ACAD OF AGRI SCI
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