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50 results about "Potassium ion uptake" patented technology
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Potassium ion channel protein KC1-D as well as encoding gene and application thereof
ActiveCN105524157AImprove tolerancePromote absorptionPlant peptidesFermentationWAS PROTEINPotassium ions
The invention discloses potassium ion channel protein KC1-D as well as an encoding gene and an application thereof. The provided protein is named as KC1-D protein and is protein constituted by amino acid sequences in a sequence 1 in a sequence table. A gene (KC1-D gene) for encoding the KC1-D protein also belongs to the protection range of the invention. The invention further protects a method for culturing a transgenic plant. The KC1-D gene is introduced into a target plant, and the transgenic plant is obtained; the transgenic plant meets at least one phenotype from (d1) to (d3): (d1) the potassium ion absorbing ability is higher than that of the target plant; (d2) the potassium ion absorbing speed is higher than that of the target plant; (d3) the stress tolerance to the low-potassium environment is higher than that of the target plant. The potassium ion channel protein KC1-D has higher application value in improvement of crop molecules and has important theoretical significance and practical significance in culture of crops tolerant to low-potassium stress.
Owner:CHINA AGRI UNIV
Tobacco HKT 1 gene and preparation method and application thereof
The invention provides a tobacco HKT1 gene. The sequence of the tobacco HKT 1 gene is shown in Seq ID No.1. A preparation method of the HKT1 gene comprises the followings steps of designing PCR amplification primers; extracting total RNA of tobacco cells; synthesizing tobacco cell cDNA; using the tobacco cell cDNA as a template to perform PCR amplification of the HKT1 gene and obtaining a target fragment, and after sequencing, obtaining the HKT1 gene sequence. The total length of the HKT1 gene is 1488 bp. After functional verification, the HKT1 gene is transferred into a potassium absorption defect type yeast mutant R5421, the recombinant yeast has absorption and transport functions. HKT1 gene overexpression in tobacco plants can promote adsorption for potassium ions of tobaccos, and the HKT1 gene has the functions of promoting adsorption and transport of the potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
Protein for promoting efficient absorption of potassium ions and coding gene thereof
ActiveCN110078807AStrong absorption capacityImprove salt toleranceAntibody mimetics/scaffoldsClimate change adaptationPotassium ionsPotassium ion uptake
The invention discloses protein for promoting efficient absorption of potassium ions and a coding gene thereof. The protein for promoting efficient absorption of the potassium ions disclosed by the invention comprises the following protein A1, A2 or A3: A1, protein with the amino acid sequence being sequence 1; A2, proteins with the amino acid sequence shown as the sequence 1 in a sequence table subjected to substitution and / or deletion and / or addition by one or several amino acid residues, and with identical functions; A3, fused protein obtained by connecting labels at the N end and / or C endof the A1 or A2. When the protein provided by the invention is expressed in plants, the potassium ion absorption capability and the salt tolerance of the plants can be improved; compared with HAK family genes of the other plants, the protein shows high potassium absorption capability under the low-potassium condition. The result shows that the protein and the coding gene of the protein disclosed by the invention can promote the efficient absorption of the potassium ions and have wide application prospects.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Tobacco KC1 gene, and preparation method and application thereof
PendingCN107012152APromote absorptionEasy to transportPlant peptidesFermentationYeastAgricultural science
The invention provides a KC1 gene. A sequence is shown as Seq ID No. 1. A preparation method of the KC1 gene comprises the following steps: designing a PCR amplification primer; extracting tobacco cell total RNA; synthesizing a tobacco cell cDNA; and performing PCR amplification for the KC1 gene by adopting the tobacco cell cDNA as a template to obtain a target fragment, and sequencing to obtain a KC1 gene sequence. A full length of the KC1 gene is 1923bp, by virtue of functional verification, a recombinant yeast after the KC1 gene is transferred into a potassium absorption-defect yeast mutant strain R5421 has a potassium ion absorption and transferring function, the over-expressed KC1 gene in a tobacco plant can promote the absorption of the tobacco to the potassium ions. The KC1 gene of the invention has a function for facilitating the absorption and transferring of the potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
Method for measuring potassium ion absorption kinetics of cotton
ActiveCN105675856AAccelerate the effect of effluxReduce effluxColor/spectral properties measurementsBiological testingAbsorption kineticsPhysical chemistry
The invention relates to the technical field of agriculture, in particular to a method for measuring potassium ion absorption kinetics of cotton. The method specifically comprises the steps that a cotton seedling is fixed in depletion liquid, and during depletion treatment of 0-1 h, a depletion liquid sample is taken once every 5-10 min; during depletion treatment of 1-h, the depletion liquid sample is taken once every 30-60 min; during depletion treatment of 10-30h, the depletion liquid sample is taken once every 2-4 h, and the concentrations of potassium ions in the depletion liquid samples are measured respectively. Related parameters in the experiment process are optimized, especially the sampling time interval is optimized, and a drawn absorption curve can reflect the potassium ion absorption law of the cotton more precisely. The method is easy to operate and high in precision, the absorption kinetics curve is smooth, and a theoretical basis is provided for accurate screening of the efficient gene type cotton and reasonable application of potash fertilizer.
Owner:INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Tobacco AKT1 gene and application
Owner:GUIZHOU TOBACCO SCI RES INST
Nucleic acid sequences of novel wheat potassium channel beta subunit KTB and KHB genes and the application thereof
InactiveCN101565704AIncrease profitImprove conversion ratePlant peptidesFermentationBiotechnologyPotassium ions
The invention discloses a novel wheat voltage gated potassium channel beta subunit encoding genes KTB and KHB (respectively from wheat and barley). Shown in sequence tables SEQ ID NO.1 and SEQ ID NO.2, the wheat crops absorb and transfer potassium ions in the different culture process, which can be evaluated through the gene sequences, and expression products can be used for presuming association of the potassium ions in an absorbing and transferring channel and related function substances. Meanwhile, the genes are transferred into the wheat crops by utilizing a transgene technology so as to obviously improve low potassium dgermination of the wheat crops. For improvement of utilization efficiency on potassium fertilizer, the invention has important practical value and theoretical significance.
Owner:SICHUAN AGRI UNIV
Tobacco KUP8 protein and encoding gene and application thereof
ActiveCN109369786APromote absorptionFunction increasePlant peptidesFermentationYeastNicotiana tabacum
The invention relates to the field of genetic engineering and particularly discloses a tobacco KUP8 protein and an encoding gene and application thereof. A KUP8 gene separated from tobaccos is provided for the first time, a nucleotide sequence of the KUP8 gene is shown as SEQ ID NO.2, and the encoded nucleotide sequence of the KUP8 gene is shown as SEQ ID NO.1. It is shown through functional identification that the provided KUP8 gene is transferred into a potassium absorption deficient yeast mutant strain R5421, then a recombinant yeast expressing the KUP8 gene has functions of potassium ion absorption and transport. It is explained that the KUP8 gene provided by the invention has the function of promoting the absorption and transport of potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
Potassium transport protein TPK1 from tobacco and coded gene and application thereof
The invention relates to the field of genetic engineering, and particularly discloses a potassium transport protein TPK1 from tobacco and a coded gene and application thereof. A TPK1 gene separated from the tobacco is provided for the first time, the full length of the TPK1 gene is 1062 bp, functional verification proves that after the provided TPK1 gene is transferred to a potassium uptake defected type yeast mutant strain R5421, the function of potassium ion absorbing and transferring is regained by a recombinant yeast expressing the TPK1 gene, and therefore the provided TPK1 gene has the function of promoting potassium ion absorbing and transferring.
Owner:GUIZHOU TOBACCO SCI RES INST
Tobacco KUP7 protein as well as coding gene and application thereof
The invention relates to the field of gene engineering and particularly discloses tobacco KUP7 protein as well as a coding gene and application thereof. The invention firstly provides the KUP7 gene separated from tobacco, wherein the nucleotide sequence of the KUP7 gene is shown as SEQ ID NO.2 and the amino acid sequence of the encoded protein KUP7 is shown as SEQ ID NO.1. Through functional verification, after the KUP7 gene provided by the invention is transferred into potassium absorption auxotrophic yeast mutant strain R5421, recombinant yeast expressing the KUP7 gene has the functions of absorbing and transporting potassium ions. The KUP7 gene provided by the invention is proved to have the function of promoting the absorption and transportation of the potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
Tobacco AKT2/3 gene and application
The invention relates to a tobacco AKT2 / 3 gene and an application. The tobacco AKT2 / 3 gene and the coded protein sequence are shown as SEQ ID NO: 1 and 2 respectively. The AKT2 / 3 gene is cloned from tobacco for the first time, and the biological function of the AKT2 / 3 gene is verified through a complementary experiment of yeast function. The AKT2 / 3 gene can promote the absorption and transport ofpotassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
SNP molecular markers linked with rice sodium and potassium ion absorption QTL and application thereof
PendingCN111733278AImprove purposeHigh linkageMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyMolecular breeding
The invention relates to the field of genetic breeding, and particularly relates to SNP molecular markers linked with rice sodium and potassium ion absorption QTL qK / Na-3-1, primers and application. The SNP molecular markers RS (36172492) and RS (36174628) are located on a Chr3 chromosome short arm of an IRGSP-1.0 genome version, the sequences are shown as SEQ ID NO. 1 and SEQ ID NO. 2, the polymorphisms are (T / G) (G / -), (A / -), and the SNP molecular markers can be obtained by amplifying the primers shown as SEQ ID NO. 3 and SEQ ID NO. 5. The molecular markers can accurately track the rice sodium and potassium ion absorption QTL qK / Na-3-1 and predict the potential salt tolerance characteristic of rice, and then molecular breeding is facilitated. The invention further discloses a method foridentifying the rice sodium and potassium ion absorption QTL qK / Na-3-1 molecular markers. By means of the method, the accuracy of sodium and potassium ion absorption prediction can be enhanced, so that rice varieties or strains with potential enhanced rice salt tolerance are quickly screened out for breeding, and the breeding process of the rice varieties with potential salt tolerance can be greatly accelerated.
Owner:SHANGHAI ACAD OF AGRI SCI
Application and expression vectors of paddy rice stomata open type potassium ion channel gene OsK2-1
ActiveCN109082428AImprove effective utilizationStomatal opening increasedPlant peptidesFermentationAgricultural scienceINCREASED EFFECT
The invention relates to application and expression vectors of a paddy rice stomata open type potassium ion channel gene OsK2-1. The paddy rice stomata open type potassium ion channel gene OsK2-1 is obtained through cloning. The gene is mainly expressed in paddy rice overground part mesophyll and stomatal guard cells, and the gene protein has transport activity for mediating potassium ion inward absorption. The invention discovers the application of the paddy rice stomata open type potassium ion channel gene OsK2-1 in the increasing of crop photosynthetic rate, stomatal conductance and transpiration rate and in the increasing of nitrogen utilization efficiency in crop high-nitrogen environments for the first time. The animal expression vector and the plane overexpression vector of the paddy rice stomata open type potassium ion channel gene OsK2-1 evidently increase the biomass and grain yield of paddy rice modified by the OsK2-1 gene and especially has a good yield increasing effect and promising application prospect under a high-nitrogen condition.
Owner:INST OF SOIL SCI CHINESE ACAD OF SCI
Tobacco kup2 gene and its application
The invention relates to tobacco KUP2 gene and its application. The sequences of the tobacco KUP2 gene and its encoded protein are shown in SEQ ID NO: 1 and 2, respectively. The present invention clones the KUP2 gene from tobacco for the first time, and verifies the biological function of the gene through the yeast function complementation experiment. The tobacco KUP2 gene has the function of promoting potassium ion absorption and transport.
Owner:GUIZHOU TOBACCO SCI RES INST
Tobacco KC1 gene and application thereof
The present invention relates to a tobacco KC1 gene and an application thereof. The tobacco KC1 gene and a sequence thereof for encoding proteins are respectively shown as SEQ ID NOS: 1 and 2. The KC1gene is obtained by cloning from tobaccos for the first time, biological functions of the gene are verified by yeast functional complementation experiments, and the tobacco KC1 gene has functions ofpromoting potassium ion absorption and transport.
Owner:GUIZHOU TOBACCO SCI RES INST
A potassium transporter kup9 from tobacco and its coding gene and application
The invention relates to the field of genetic engineering, and specifically discloses a potassium transporter KUP9 from tobacco, its coding gene and application. The present invention provides for the first time the KUP9 gene isolated from tobacco. The full length of the KUP9 gene is 2370 bp. After functional verification, the KUP9 gene provided by the present invention is transferred into the potassium absorption-deficient yeast mutant strain R5421, and then expresses the recombinant yeast of the KUP9 gene. It regained the function of potassium ion absorption and transport. Therefore, the KUP9 gene provided by the present invention has the function of promoting the absorption and transport of potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
Tobacco KUP5 protein and coding gene and application thereof
The invention relates to the field of genetic engineering, and specifically discloses tobacco KUP5 protein and a coding gene and application thereof. The tobacco KUP5 protein and the coding gene and application thereof have the advantages that a KUP5 gene isolated from tobacco is provided for the first time, the nucleotide sequence of the KUP5 gene is shown in SEQ ID NO.2, and the amino acid sequence of the KUP5 protein encoded by the KUP5 gene is shown in SEQ ID NO.1; through functional verification, after the KUP5 gene is transferred into a potassium-absorptive deficit type yeast mutant strain R5421, the recombined yeast expressing the KUP5 gene has potassium ion absorption and transport functions, that is to say, the KUP5 gene has the function of promoting the absorption and transport of potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
Method for cultivating low-potassium-tolerant rice
The invention discloses a method for cultivating low-potassium-tolerant rice. The rice OsSPL19 gene is directionally knocked out by using a CRISPER / Cas9 technology, and it is found that a transgenic plant with the OsSPL19 gene knocked out is sensitive to low potassium, the plant is short and small under low potassium treatment, the root is shortened, the potassium ion content of the root and the overground part is obviously reduced, and the potassium ion absorption capacity of the root is obviously reduced. Overexpression of the OsSPL19 gene in rice shows that a plant overexpressed by the OsSPL19 gene is tolerant to low potassium and grows well under a low-potassium condition, the potassium ion content of plant roots and overground parts is obviously increased, and the potassium ion absorption capacity of the roots is obviously enhanced. The result shows that the OsSPL19 gene participates in a rice low-potassium-resistant signal pathway and is a rice low-potassium-resistant gene. According to the invention, guarantee can be provided for improving the low-potassium resistance of the rice and cultivating low-potassium-resistant rice varieties.
Owner:HUNAN INST OF MICROBIOLOGY
Application of cotton gene GhEIN3dD in improving low potassium tolerance of plants
The invention relates to the field of plant breeding, in particular to application of a GhEIN3dD gene in promoting low potassium stress tolerance of plants, a recombinant vector, a recombinant microorganism, a method for improving the low potassium stress tolerance of the plants and a plant breeding method. According to the application of the GhEIN3dD gene in promoting the low potassium stress tolerance of the plant, the GhEIN3dD gene in the plant is over-expressed to improve the low potassium stress tolerance of the plant and / or improve the potassium ion absorption capacity of the plant, and the nucleotide sequence of the GhEIN3dD gene is shown as SEQ ID NO: 1. According to the application of the GhEIN3dD gene in promoting the low potassium stress resistance of the plant provided by the embodiment of the invention, the low potassium stress resistance of the plant can be improved in a low potassium environment in which a trace amount of K < + > is exogenously added, and the growth of the plant is remarkably promoted.
Owner:CHINA AGRI UNIV
Tobacco KUP2 gene and applications thereof
The present invention relates to a tobacco KUP2 gene and applications thereof. The sequences of the tobacco KUP2 gene and a protein encoded by the gene are respectively shown in SEQ ID NO:1 and SEQ IDNO:2. The KUP2 gene is obtained by cloning from tobacco for the first time, and the biological functions of the gene are verified by a yeast functional complementation experiment. The tobacco KUP2 gene has the function of promoting potassium ion absorption and transport.
Owner:GUIZHOU TOBACCO SCI RES INST
A potassium transporter kup3 from tobacco and its coding gene and application
ActiveCN109354611BPromote absorptionFunction increasePlant peptidesFermentationYeastNicotiana tabacum
The invention relates to the field of genetic engineering, and specifically discloses a potassium transporter KUP3 from tobacco, its coding gene and its application. The present invention provides for the first time the KUP3 gene isolated from tobacco. The full length of the KUP3 gene is 2364bp. After functional verification, the KUP3 gene provided by the present invention is transferred to the potassium absorption-deficient yeast mutant strain R5421, and the recombinant yeast expressing the KUP3 gene It regained the function of potassium ion absorption and transport. Therefore, the KUP3 gene provided by the present invention has the function of promoting the absorption and transport of potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
Application of MYC transcription factor related biological product in improving low potassium resistance of upland cotton
The invention relates to the technical field of plant breeding, in particular to application of MYC transcription factor related biological products to improvement of low potassium resistance of upland cotton. The MYC type transcription factor comprises GhMYC2bA or / and GhMYC2dA, and the MYC type transcription factor comprises GhMYC2bA or / and The base sequence of the GhMYC2bA gene for coding the GhMYC2bA type transcription factor is as shown in SEQ ID NO. 1 (sequence identifier number 1). The GhMYC2bA or GhMYC2dA gene in the cotton is overexpressed through a gene engineering method, the adaptive capacity of the cotton in a low-potassium environment can be remarkably enhanced, and the expression is that the plant growth is improved, the dry matter accumulation is increased, the potassium ion absorption efficiency is improved, and the leaf yellowing symptom is relieved. The invention provides a new gene target and a new breeding strategy for cultivating a low-potassium-tolerant cotton variety, and has important significance for solving the problem of potassium deficiency in farmland and ensuring stable and high yield of cotton.
Owner:CHINA AGRI UNIV
Tobacco kup1 gene and its application
Owner:GUIZHOU TOBACCO SCI RES INST
A kind of tobacco kup7 protein and its coding gene and application
The invention relates to the field of genetic engineering, and specifically discloses a tobacco KUP7 protein and its coding gene and application. The present invention provides for the first time the KUP7 gene isolated from tobacco. The nucleotide sequence of the KUP7 gene is shown in SEQ ID NO.2, and the amino acid sequence of the protein KUP7 encoded by it is shown in SEQ ID NO.1. After functional verification, After the KUP7 gene provided by the invention is transferred into the potassium absorption-deficient yeast mutant strain R5421, the recombinant yeast expressing the KUP7 gene has the functions of potassium ion absorption and transport. It shows that the KUP7 gene provided by the present invention has the function of promoting the absorption and transport of potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
A potassium transporter tpk1-1 from tobacco and its coding gene and application
The invention relates to the field of genetic engineering, and specifically discloses a potassium transporter TPK1-1 from tobacco, its coding gene and its application. The present invention provides the TPK1-1 gene isolated from tobacco for the first time. The full length of the TPK1-1 gene is 1287 bp. After functional verification, the TPK1-1 gene provided by the present invention is transferred into the potassium absorption-deficient yeast mutant strain R5421, and expresses the TPK1-1 gene. The recombinant yeast with the TPK1‑1 gene has regained the functions of potassium ion uptake and transport. Therefore, the TPK1-1 gene provided by the present invention has the function of promoting the absorption and transport of potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
A method for measuring cotton potassium ion absorption kinetics
ActiveCN105675856BMethods to refine absorption kineticsReflects the absorption of potassium ionsColor/spectral properties measurementsBiological testingAbsorption kineticsPhysical chemistry
Owner:INST OF COTTON RES CHINESE ACAD OF AGRI SCI
A kind of tobacco kup5 protein and its coding gene and application
ActiveCN109354610BPromote absorptionEasy to transportPlant peptidesFermentationNicotiana tabacumNucleotide sequencing
The invention relates to the field of genetic engineering, and specifically discloses a tobacco KUP5 protein, its coding gene and its application. The present invention provides for the first time the KUP5 gene isolated from tobacco. The nucleotide sequence of the KUP5 gene is shown in SEQ ID NO.2, and the amino acid sequence of the protein KUP5 encoded by it is shown in SEQ ID NO.1. After functional verification, After the KUP5 gene provided by the invention is transferred into the potassium absorption-deficient yeast mutant strain R5421, the recombinant yeast expressing the KUP5 gene has the functions of potassium ion absorption and transport. It shows that the KUP5 gene provided by the present invention has the function of promoting the absorption and transport of potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST
Potassium transport protein KUP3 from tobacco and coded gene and application thereof
The invention relates to the field of genetic engineering, and particularly discloses a potassium transport protein KUP3 from tobacco and a coded gene and application thereof. A KUP3 gene separated from the tobacco is provided for the first time, the full length of the KUP3 gene is 2364 bp, functional verification proves that after the provided KUP3 gene is transferred to a potassium uptake defected type yeast mutant strain R5421, the function of potassium ion absorbing and transferring is regained by a recombinant yeast expressing the KUP3 gene, and therefore the provided KUP3 gene has the function of promoting potassium ion absorbing and transferring.
Owner:GUIZHOU TOBACCO SCI RES INST
Potassium translocator KUP11 from tobacco and coding gene and application thereof
The invention relates to the field of genetic engineering, and specifically discloses a potassium translocator KUP11 from tobacco and a coding gene and application thereof. The KUP11 gene separated from the tobacco is provided for the first time. The KUP11 gene is 2391bp in length. According to functional verification, after the KUP11 gene is transferred into the potassium absorption deficient yeast mutant R5421, recombinant yeast expressing the KUP11 gene has the functions of potassium ion absorption and transportation again. Therefore, the KUP11 gene has the functions of promoting potassiumion absorption and transportation.
Owner:GUIZHOU TOBACCO SCI RES INST
A kind of tobacco kup6 protein and its coding gene and application
ActiveCN109438564BFunctionalPromote absorptionPlant peptidesFermentationNicotiana tabacumNucleotide sequencing
The invention relates to the field of genetic engineering, and specifically discloses a tobacco KUP6 protein, its coding gene and its application. The present invention provides the KUP6 gene isolated from tobacco for the first time. The nucleotide sequence of the KUP6 gene is shown in SEQ ID NO.2, and the amino acid sequence of the protein KUP6 encoded by it is shown in SEQ ID NO.1. After functional verification, After the KUP6 gene provided by the invention is transferred into the potassium absorption-deficient yeast mutant strain R5421, the recombinant yeast expressing the KUP6 gene has the functions of potassium ion absorption and transport. It shows that the KUP6 gene provided by the present invention has the function of promoting the absorption and transport of potassium ions.
Owner:GUIZHOU TOBACCO SCI RES INST