Application and expression vectors of paddy rice stomata open type potassium ion channel gene OsK2-1

Abstract

The invention relates to application and expression vectors of a paddy rice stomata open type potassium ion channel gene OsK2-1. The paddy rice stomata open type potassium ion channel gene OsK2-1 is obtained through cloning. The gene is mainly expressed in paddy rice overground part mesophyll and stomatal guard cells, and the gene protein has transport activity for mediating potassium ion inward absorption. The invention discovers the application of the paddy rice stomata open type potassium ion channel gene OsK2-1 in the increasing of crop photosynthetic rate, stomatal conductance and transpiration rate and in the increasing of nitrogen utilization efficiency in crop high-nitrogen environments for the first time. The animal expression vector and the plane overexpression vector of the paddy rice stomata open type potassium ion channel gene OsK2-1 evidently increase the biomass and grain yield of paddy rice modified by the OsK2-1 gene and especially has a good yield increasing effect and promising application prospect under a high-nitrogen condition.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the application of a rice stomatal open type potassium ion channel protein gene Osk2-1. Background technique [0002] Potassium is a key mineral nutrient element necessary for the growth and development of crops. It not only has the functions of improving the activity of enzyme systems in plants, promoting plant metabolism, material transportation in vivo and protein synthesis, but also can enhance plant photosynthesis and plant stress resistance. sex. Potassium uptake by plants is primarily mediated by various classes of potassium transporters and potassium channels (Epstein et al., 1963). The main feature of the transporter is that it combines with the transported ion and realizes transmembrane transport through a series of conformational changes. So far, three families of potassium transporters have been found in plants, namely KUP / HAK / KT family, TRK / HKT f...

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Problems solved by technology

Existing studies on the improvement of nitrogen nutritional shape in rice by overexpressing ammonium uptake assimilation or response genes by means of molecular regulation, overexpressing the rice ammonium uptake system OsAMT1; 4 + (much lower than the actual farmland ammonium level) showed some positive effects, at higher ammonium (3mM NH 4 + ) under the condition of rice growth inhibition phenomenon (Ranathunge et al., 2014); regulation of another rice ammonium uptake system OsAMT1; Rice plants expressing ammonium assimilation genes such as OsGS1;1,1;2 and glnA all had reduced yield (Cai et al., 2009)

Overexpression of the nitrogen metabolism regulatory gene Dof1, a transcription factor derived from maize, can improve the nitrogen use efficiency of rice, but this positive effect only appears under low nitrogen conditions (Kurai et al., 2011)

Some other transcription factors found in recent years, such as HAP3, 14-3-3 protein, qNGR9, MiRNA, and kinases CIPK8 and CIPK23 are involved in the regulation of nitrogen uptake metabolism, but it has not been seen that these genes are involved in improving nitrogen use efficiency in rice. Applications

And the effective genes found in the application are all effective under the condition of low nitrogen supply, which does not match the current highly intensive production mode in my country

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