Tobacco KUP7 protein as well as coding gene and application thereof

Abstract

The invention relates to the field of gene engineering and particularly discloses tobacco KUP7 protein as well as a coding gene and application thereof. The invention firstly provides the KUP7 gene separated from tobacco, wherein the nucleotide sequence of the KUP7 gene is shown as SEQ ID NO.2 and the amino acid sequence of the encoded protein KUP7 is shown as SEQ ID NO.1. Through functional verification, after the KUP7 gene provided by the invention is transferred into potassium absorption auxotrophic yeast mutant strain R5421, recombinant yeast expressing the KUP7 gene has the functions of absorbing and transporting potassium ions. The KUP7 gene provided by the invention is proved to have the function of promoting the absorption and transportation of the potassium ions.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a potassium transporter KUP7 from tobacco and its coding gene and application. Background technique [0002] Potassium transporter is a carrier protein that can transport potassium ions into cells when the external potassium ion concentration is extremely low. Potassium transporters are usually some transmembrane proteins, which complete the transmembrane transport of potassium ions through conformational changes. It is an active transport process that requires ATP to provide energy, usually K + / H + or K + / Na + Collaborative transport. [0003] Existing technology studies on potassium transporter genes are extensive in the model plant Arabidopsis thaliana. For example, studies have shown that Arabidopsis mutant kup6 has a lower survival rate under drought stress than wild-type plants, while 35S::KUP6 overexpression The drought tolerance of transgenic plants was significa...

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Problems solved by technology

[0003] Existing technology studies on potassium transporter genes are extensive in the model plant Arabidopsis thaliana. For example, studies have shown that Arabidopsis mutant kup6 has a lower survival rate under drought stress than wild-type plants, while 35S::KUP6 overexpression The drought tolerance of transgenic plants was significantly enhanced (Shabala et al., 2007); while the mutant seedlings of KUP7 gene exhibited a low-potassium-sensitive phenotype of yellowing leaves under low-potassium stress, and the potassium ions in the roots The content decreased significantly, and the loss of KUP7 gene function reduced the ability of Arabidopsis seedlings to absorb potassium under low potassium conditions, and the concentration of potassium ions in xylem juice decreased (Min et al., 2016)

[0004] Tobacco is a crop that consumes a lot of potassium. The potassium content of tobacco leaves is an important indicator to measure the quality of tobacco leaves. At present, there are few studies on potassium transporter genes in tobacco, and the function of tobacco KUP is unknown.

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