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Method for measuring potassium ion absorption kinetics of cotton

A potassium ion and kinetic technology, applied in the agricultural field, can solve the problems of rough and inaccurate measurement methods, difficult measurement of cotton plants, etc., and achieve the effects of improving the efflux effect, optimizing the sampling duration, and reducing the concentration of K+ ions.

Active Publication Date: 2016-06-15
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0005] The purpose of the present invention is to provide an improved method for measuring cotton potassium ion absorption kinetics, which adjusts relevant experimental parameters in the determination process, aiming at defects such as large cotton plants are difficult to measure, and existing measurement methods are rough and inaccurate. , the experimental conditions for forming different types of absorption curves were established

Method used

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  • Method for measuring potassium ion absorption kinetics of cotton
  • Method for measuring potassium ion absorption kinetics of cotton
  • Method for measuring potassium ion absorption kinetics of cotton

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The determination of potassium ion uptake kinetics of cotton seedlings in the first leaf stage of embodiment 1

[0039] Starvation treatment: choose to provide sufficient potassium 2.5mmolL -1 (High Potassium) conditions obtained under the one-leaf stage, 6 representative cotton seedlings with consistent growth (the difference in weight between groups is not more than 2g), 3 plants are used as a repetition (3 repetitions), and the roots of the seedlings are deionized. Rinse well with water and dry with absorbent paper. Take the triangular flask, put 600mL starvation solution (5mmolL -1 MES, 200μmolL -1 Ca 2 SO 4 , pH=6.8), wrapped the joints of the seedlings’ rhizomes with sponges, fixed them in the Erlenmeyer flask for 24h of starvation treatment (changed the starvation solution every 8h); Keep ventilation in the Erlenmeyer flask.

[0040] Depletion treatment: After 24 hours of starvation treatment, the cotton seedlings were taken out from the starvation solution...

Embodiment 2 2

[0042] The determination of potassium ion uptake kinetics of cotton seedlings in the second leaf stage of embodiment 2

[0043] The operation method of this example is the same as that of Example 2, the only difference is that the concentration of potassium ions in the depletion solution and the age of the seedlings are different. The concentrations of potassium ions in the depletion solution of this example are: 162 μmolL -1 , 204 μmol L -1 , 252 μmol L -1 , the seedling age is the two-leaf stage seedlings, the absorption kinetic curve obtained by drawing is as follows figure 2 shown.

Embodiment 3 3

[0044] Potassium ion uptake kinetics determination of embodiment 3 three-leaf stage cotton seedlings

[0045] The operating method of the present embodiment is the same as that of Example 2, and the difference is that the materials used in the experiment are different; the materials used in the present embodiment are: under low potassium stress 0.05mmolL -1 (low potassium) and sufficient potassium supply 2.5mmolL -1 Cotton seedlings cultivated to the three-leaf stage under (high potassium) conditions; the absorption kinetic curve obtained by drawing is as follows image 3 shown.

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Abstract

The invention relates to the technical field of agriculture, in particular to a method for measuring potassium ion absorption kinetics of cotton. The method specifically comprises the steps that a cotton seedling is fixed in depletion liquid, and during depletion treatment of 0-1 h, a depletion liquid sample is taken once every 5-10 min; during depletion treatment of 1-h, the depletion liquid sample is taken once every 30-60 min; during depletion treatment of 10-30h, the depletion liquid sample is taken once every 2-4 h, and the concentrations of potassium ions in the depletion liquid samples are measured respectively. Related parameters in the experiment process are optimized, especially the sampling time interval is optimized, and a drawn absorption curve can reflect the potassium ion absorption law of the cotton more precisely. The method is easy to operate and high in precision, the absorption kinetics curve is smooth, and a theoretical basis is provided for accurate screening of the efficient gene type cotton and reasonable application of potash fertilizer.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for measuring cotton potassium ion absorption kinetics. Background technique [0002] Potassium is one of the three elements of crop nutrition. Compared with soil available nitrogen and phosphorus, the content of available potassium in agricultural soil in my country is relatively rich. Originally, the problem of potassium deficiency was not very prominent, but with the development of agricultural production, crop yields increased , the amount of nutrients taken away increased, the nutrients returned to the soil decreased sharply, the application of traditional farmyard manure and plant ash decreased, and the application of nitrogen and phosphorus fertilizers increased, resulting in nutrient imbalance and potassium deficiency in varying degrees. There are mainly two ways to alleviate the shortage of potassium sources in my country: on the one hand, relying...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/31
CPCG01N21/3103G01N33/5097
Inventor 王宁许庆华杨杰苏桂兰周红黄群严根土
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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