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Molecular marker for efficient utilization of QTL site by barley phosphorus and application of marker

A molecular marker, barley phosphorus technology, applied in the field of molecular biology, can solve the problems of different phosphorus absorption and utilization capacity and yield contribution difference, and achieve the effect of increasing phosphorus utilization efficiency, high accuracy, and improving selection and identification efficiency.

Inactive Publication Date: 2019-06-21
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that different genotypes of barley have different abilities to absorb and utilize phosphorus, and their contributions to yield also vary

Method used

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  • Molecular marker for efficient utilization of QTL site by barley phosphorus and application of marker
  • Molecular marker for efficient utilization of QTL site by barley phosphorus and application of marker
  • Molecular marker for efficient utilization of QTL site by barley phosphorus and application of marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Mapping of QTL site Qspue.sau-3H.01 for high phosphorus utilization in barley and acquisition of molecular marker Hvc630

[0038] 1) The cultivated barley Baudin was used as the female parent, and the phosphorus efficient utilization genotype CN4027 was used as the male parent to obtain the hybrid F 1 , F 1 F 2 , using the single seed method to obtain F with 128 lines 8 Generation RIL population constitutes genetic mapping population;

[0039] 2) Using the CTAB method to extract the DNA of each strain of the genetic mapping population and its parents, using DArT chip technology, using the DNA of the parents Baudin and CN4027 as templates, genotyping to obtain the genotype of the RIL population material.

[0040] The band pattern of the parent Baudin is marked as a, and the band pattern of the parent CN4027 is marked as b. f 8 The zonation type of population strains derived from Baudin is denoted as a, and that derived from CN4027 is denoted as b;

[004...

Embodiment 2

[0054] Example 2 Application of Molecular Marker Hvc630 Tightly Linked to Barley Phosphorus Efficient Utilization QTL Site Qspue.sau-3H.01

[0055] 1. Extraction of DNA

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Abstract

The invention discloses a molecular marker for efficient utilization of a QTL site by barley phosphorus and an application of the marker. The molecular marker is Hvc630, a nucleotide sequence of the marker is shown in SEQ ID NO: 1 and is positioned on a barley 3H chromosome, and thus the marker can accurately track the QTL site efficiently utilized by the barley phosphorus, predict barley phosphorus utilization efficiency characteristics, and further facilitate molecular design breeding for nutrient efficient utilization. The invention further provides the application of the molecular marker Hvc630 in barley breeding with high-yield high-efficiency nutrients. By using the method, the accuracy of barley phosphorus utilization efficiency prediction can be enhanced, and barley varieties or strains with QTL for improving barley phosphorus utilization efficiency can be rapidly screened for breeding, so that the breeding process of high-yield high-efficiency barley varieties can be greatly accelerated.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, relates to plant nutrition and barley molecular breeding, in particular to a molecular marker and application of barley phosphorus efficient utilization QTL site. Background technique [0002] Barley (Hordeum vulgare L.) is one of the important cereal crops, planted all over the world, ranking fifth in the world's crop production, with a planting area of ​​about 560,000 km 2 , the total output is about 120 million metric tons, and the planting area in my country is about 24.38 million mu. [0003] Phosphorus is an important guarantee for high-yield and high-quality crops, and its status in agricultural production is increasingly prominent. The low content of available phosphorus in the soil is one of the important factors that limit the increase of crop yield. According to reports, 30% to 40% of the cultivated land in the world is severely inhibited due to soil phosphorus deficiency. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 陈光登李雨航陈卉儒陈柯胡德益刘亚西马建张森峰陈晨高铱遥袁澍
Owner SICHUAN AGRI UNIV
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