Molecular marker MAG7237 of wheat fusarium head blight infection resistant gene Fhb4 and application of molecular marker MAG7237

A technology of MAG7237 and MAG7237-F, which is applied in the field of molecular markers of wheat head blight resistance gene Fhb4, can solve the problems of large genetic distance and speed up gene cloning research, and achieve stable amplification, convenient identification, and convenient detection Effect

Inactive Publication Date: 2014-07-02
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

In addition, although Fhb4 has been finely mapped, the genetic distance in the interval is still very large. It is necessary to develop more closely linked molecular markers to speed up the cloning research of this gene

Method used

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  • Molecular marker MAG7237 of wheat fusarium head blight infection resistant gene Fhb4 and application of molecular marker MAG7237
  • Molecular marker MAG7237 of wheat fusarium head blight infection resistant gene Fhb4 and application of molecular marker MAG7237
  • Molecular marker MAG7237 of wheat fusarium head blight infection resistant gene Fhb4 and application of molecular marker MAG7237

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Experimental program
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Effect test

Embodiment 1

[0044] The molecular marker of wheat head blight resistance gene Fhb4 was obtained by the following methods:

[0045] (1) Wangshuibai Fhb4 near-isogenic line NMAS007 and its recurrent parent Mianyang 99-323F 2：3 Creation of populations and screening of Fhb4 segment recombinants:

[0046] (1) NMAS007 (♀) and wheat variety Mianyang 99-323 hybrid F 1 , F 1 Selfing produced F containing 4303 individuals 2 group;

[0047] (2) Using the border markers Xhbg226 and Xgwm149 of Fhb4 (Torada et al.2006; et al.1998) in F 2 In the population, 187 heterozygous individuals with recombination in this segment were screened, and the same marker was used in its F 3 Generation screening yielded 459 homozygous individuals recombined in this segment.

[0048] (2) Identification of recombinant disease-resistant phenotypes

[0049] Sow susceptible wheat grains in the field about ten days before the recombinants flower to inoculate these homozygous recombinants, and repeat once a week late...

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Abstract

The invention belongs to the field of breeding of plants and discloses a molecular marker MAG7237 of a wheat fusarium head blight infection resistant gene Fhb4 and application of the molecular marker MAG7237. The genetic distance between the molecular marker MAG7237 and the Fhb4 gene is 0.34 cm. The molecular marker most closely linked with the Fhb4 is acquired by the invention for the first time in the world. The molecular marker is a co-dominant marker with the advantages of being convenient to detect, and stable and simple to amplify; by adopting the marker MAG7237 to detect the Fhb4 gene, the existence of the Fhb4 or not and the existence state can be determined, and the fusarium head blight resistance of the wheat can be forecasted; and furthermore, the plants with the Fhb4 can be fast screened and used for breeding of disease-resistant varieties.

Description

[0001] This application is a divisional application filed on March 27, 2012, entitled "Molecular marker MAG7237 of wheat head blight resistance gene Fhb4 and its application", with application number 201210082937.9. technical field [0002] The invention belongs to the field of crop breeding, and relates to a molecular marker of wheat scab infection resistance gene Fhb4 and its application. technical background [0003] Wheat is one of the most important food crops in the world, providing about 20% of human energy. Many biotic and abiotic stresses directly affect the yield and quality of wheat in wheat production. Scab is one of the most important biological adversities, which seriously affects the quality and yield of wheat. The breeding and application of disease-resistant varieties is the most economical and effective method to control scab. The discovery of disease-resistant genes is the premise and foundation of disease-resistant breeding, and the development of molecu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68A01H1/04C12N15/29C12N15/10
Inventor 马正强薛树林李国强
Owner NANJING AGRICULTURAL UNIVERSITY
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