SNP molecular marker kasp-be-kl-sau2 linked to wheat grain length major QTL and its application

A kasp-be-kl-sau2, molecular marker technology, applied in the field of crop molecular genetics and breeding, can solve the problem of not many molecular markers, and achieve the effects of accurate and efficient detection, convenient and stable amplification, and high success rate

Active Publication Date: 2022-05-24
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many closely linked molecular markers that are related to wheat grain length traits and can be used in practical molecular breeding.

Method used

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  • SNP molecular marker kasp-be-kl-sau2 linked to wheat grain length major QTL and its application
  • SNP molecular marker kasp-be-kl-sau2 linked to wheat grain length major QTL and its application
  • SNP molecular marker kasp-be-kl-sau2 linked to wheat grain length major QTL and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Acquisition of wheat grain length QTL Qkl.sau-BE-4A and its molecular marker KASP-BE-kl-sau2

[0044] (1) Using the wheat line '99E18' as the female parent and the wheat variety 'BLS1' as the male parent, hybrid F1 was obtained, F1 generation was selfed to obtain F2, and the single seed method was used in F2 to propagate to the F3 generation, and the hybrid F1 was obtained. The recombinant inbred lines of 238 individual plants constituted the genetic mapping population.

[0045] (2) Grain length phenotype identification of the F3 population: The F3 population lines were harvested, threshed, dried and phenotypic identification of the grain characters at the maturity stage of wheat. The average value was obtained, representing the grain length of the line.

[0046] (3) Using the mixed population segregation analysis (BSA), two parental pools were constructed, and two groups of lines with 30 extreme grain length differences were selected in the F3 population to ...

Embodiment 2

[0066] Example 2 Application of molecular marker KASP-BE-kl-sau2 in selection and control of grain length QTL Qkl.sau-BE-4A

[0067] (1) Using the common wheat line 'BLS1' with longer grain length as the male parent and the common wheat line 'Sumai3' as the female parent to construct F 3 population, 183 lines were selected among the progeny lines.

[0068] (2) KASP-BE-kl-sau2 marker detection was performed on the obtained 183 strains, and the specific method was as follows: DNA of 183 strains was extracted; Specific primer pairs are primers for PCR amplification and fluorescence reading, and the primers are:

[0069] Primer on FAM tag: (the underlined part is the FAM tag sequence) 5'- GAAGGTGACCAAGTTCATGCT CCAGCCGGCAGGTGGAATGCC-3' (SEQ ID NO: 4)

[0070] Primer on HEX tag: (the underlined part is the HEX tag sequence) 5'- GAAGGTCGGAGTCAACGGATT CCAGCCGGCAGGTGGAATGCT-3' (SEQ ID NO: 5)

[0071] Universal downstream primer: 5'-AAGGGGAAGGTCAGCTGCTG-3' (SEQ ID NO:6)

[0072]...

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Abstract

The invention discloses a SNP molecular marker KASP-BE-kl-sau2 linked with the main effect QTL of wheat grain length and its application, belonging to the field of crop molecular genetics and breeding. The molecular marker KASP‑BE‑kl‑sau2 polymorphism is C / T, KASP‑BE‑kl‑sau2 co-localizes with wheat grain length QTL Qkl.sau‑BE‑4A on the short arm of wheat chromosome 4A, and is located Within the Qkl.sau‑BE‑4A interval. The molecular marker KASP-BE-kl-sau2 disclosed by the present invention is extremely significantly correlated with the grain length QTLQkl.sau-BE-4A, presents the characteristics of closely linked markers, has high accuracy for molecular marker-assisted selection, and can accurately predict wheat grain length Traits play an important role in improving wheat yield and efficiently screening high-quality wheat varieties with longer grain length, and are also conducive to improving the efficiency of wheat breeding.

Description

technical field [0001] The invention relates to the technical field of crop molecular genetics and breeding, in particular to the SNP molecular marker KASP-BE-kl-sau2 linked with the main QTL of wheat grain length and its application. Background technique [0002] Common wheat (Triticum aestivum L.2n=6x=42) is one of the main crops in temperate regions, contributing essential amino acids, minerals and vitamins, as well as beneficial dietary fiber, to the human diet. Due to global climate change, population growth and massive reductions in arable land, future food demand can only be met by increasing crop yield potential. Because grain yield components, such as fertile spikelets per panicle, 1000-grain weight, and effective tiller number per unit area, generally exhibit higher heritability than grain yield, improving these factors is an important way to increase wheat grain yield potential. [0003] The reduction of genetic diversity among wheat varieties under modern breedi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 马建谢欣霖王素荣玄其京刘航唐华苹牟杨唐力为苟璐璐谭翠江千涛魏育明郑有良兰秀锦
Owner SICHUAN AGRI UNIV
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