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Rice grain width gene gw5l and its application

A technology for transgenic plants and grain types, which can be used in applications, genetic engineering, plant genetic improvement, etc., and can solve the problems of limited molecular regulation mechanism and genetic network research.

Active Publication Date: 2020-06-09
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, the research on the molecular regulation mechanism and genetic network of rice grain shape genes is very limited

Method used

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  • Rice grain width gene gw5l and its application
  • Rice grain width gene gw5l and its application
  • Rice grain width gene gw5l and its application

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0043] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0044] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0045] N6 medium was purchased from PhytoTechnology Laboratories in the United States, and the item number was C167.

[0046] Rice variety Kitaake ( Oryza sativa ) in the literature "Li Z, Wan J, Xia J, et al.Mapping of quantitative trait loci controlling physico-chemical properties of rice grains ( Oryza sativa L. ). Breeding science, 2003, 53(3): 209-215", the public can obtain it from relevant institutions and the Institute of Crop Science, Chinese Academy of Agricultural Sciences.

[0047] Agrobacterium tumefaciens EHA105 ( Agrobacterium tumefaciens EHA105) in "Hood, Elizabeth E; Gelvin, Stanton B; Melchers, Leo S; Hoekema, Andre. 1993. New Agrobacterium helper plasmads for gene transfer to plants. Transgenic rese...

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Abstract

The invention discloses a rice grain width gene GW5L and an application thereof, and provides an application of protein GW5L, a DNA molecule encoding the protein GW5L or a recombinant vector containing the DNA molecule encoding the protein GW5L in regulation of the grain shape of a plant. The gene GW5L is transferred into wild rice, and transgenic rice is obtained. Compared with recipient rice, the gene GW5L in the transgenic rice is overexpressed, the grain width of the rice is reduced substantially, and the grain length is increased significantly. A foundation is laid for cultivating transgenic plants with changed grain shapes.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to rice grain width genes GW5L Isolation of coding regions and their applications. Background technique [0002] Rice ( Oryza sativa L.) is an important food crop in the world, rice is the staple food of more than half of the world's population, and it is also the largest food crop in my country. For a long time, in order to solve the ration problem of 1.3 billion, my country's rice breeding has always focused on high yield and neglected quality improvement, resulting in the current situation of "high yield but not high quality" in my country's rice varieties. In recent years, with the rapid development of the market economy and the continuous improvement of people's living standards, the market's demand for high-quality rice has become increasingly strong, and people's requirements for the appearance of rice and the quality of cooking and eating are also increasing, especially in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10A01H6/46
Inventor 雷财林田鹏万建民刘家范赵志超王洁程治军张欣王久林郭秀平任玉龙吴赴清林启冰朱杉杉
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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