Rice grain width gene gw5l and its application
A technology for transgenic plants and grain types, which can be used in applications, genetic engineering, plant genetic improvement, etc., and can solve the problems of limited molecular regulation mechanism and genetic network research.
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[0043] The experimental methods used in the following examples are conventional methods unless otherwise specified.
[0044] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.
[0045] N6 medium was purchased from PhytoTechnology Laboratories in the United States, and the item number was C167.
[0046] Rice variety Kitaake ( Oryza sativa ) in the literature "Li Z, Wan J, Xia J, et al.Mapping of quantitative trait loci controlling physico-chemical properties of rice grains ( Oryza sativa L. ). Breeding science, 2003, 53(3): 209-215", the public can obtain it from relevant institutions and the Institute of Crop Science, Chinese Academy of Agricultural Sciences.
[0047] Agrobacterium tumefaciens EHA105 ( Agrobacterium tumefaciens EHA105) in "Hood, Elizabeth E; Gelvin, Stanton B; Melchers, Leo S; Hoekema, Andre. 1993. New Agrobacterium helper plasmads for gene transfer to plants. Transgenic rese...
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