Rice grain width gene GW5L and application thereof
A technology of transgenic plants and grain type, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of limited research on molecular regulation mechanism and genetic network
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[0044] The experimental methods used in the following examples are conventional methods unless otherwise specified.
[0045] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.
[0046] N6 medium was purchased from PhytoTechnology Laboratories in the United States, and the item number was C167.
[0047] Rice variety Kitaake ( Oryza sativa ) in the literature "Li Z, Wan J, Xia J, et al.Mapping of quantitative trait loci controlling physico-chemical properties of rice grains ( Oryza sativa L. ). Breeding science, 2003, 53(3): 209-215", and the public can obtain it from relevant institutions and the Institute of Crop Science, Chinese Academy of Agricultural Sciences.
[0048] Agrobacterium tumefaciens EHA105 ( Agrobacterium tumefaciens EHA105) in "Hood, Elizabeth E; Gelvin, Stanton B; Melchers, Leo S; Hoekema, Andre. 1993. New Agrobacterium helper plasmads for gene transfer to plants. Transgenic ...
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