Proteins related to plant type and their coding genes and applications

A protein and plant technology, applied in the field of protein and its coding gene and application, can solve the problem of unknowable regulation of plant type

Active Publication Date: 2022-05-03
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it has been found that such proteins in plants are involved in the regulation of multiple physiological and biochemical processes such as seed germination, photosynthesis, various stress responses, flowering, hormone signal transduction, etc., but the regulation of plant type is still unknown

Method used

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  • Proteins related to plant type and their coding genes and applications
  • Proteins related to plant type and their coding genes and applications

Examples

Experimental program
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Effect test

Embodiment 1

[0080] Example 1. Plant type-related protein DHT1 can regulate rice plant height, panicle length and grain width

[0081] This embodiment provides a plant type-related protein derived from rice Kitaake (Oryza sativa var. Kitaake), its name is DHT1, the amino acid sequence of DHT1 in rice Kitaake is SEQ ID No.1, the coding sequence of DHT1 gene ( That is, the CDS sequence) is SEQ ID No.2, and the genome sequence of the DHT1 gene is SEQ ID No.3.

[0082] 1. Construction of an RNA interference vector that reduces the expression of DHT1 gene

[0083] 1. Obtaining of DHT1 gene expression interference fragments

[0084] (1) Total RNA of 14-day seedlings of rice Kitaake (Oryzasativa) was extracted using RNApreppure Plant Total RNA Extraction Kit (Tiangen Biochemical Technology (Beijing) Co., Ltd.), and cDNA was obtained by reverse transcription.

[0085] (2) With the cDNA that step (1) obtains as template, carry out PCR amplification with the primer that DHT1-sense-F and DHT1-sense...

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Abstract

The invention discloses a protein related to plant type, its coding gene and application. The invention provides the application of DHT1 protein or its related biological material in regulating the development of plant grain. The relevant biological material is a nucleic acid molecule capable of expressing the DHT1 protein or an expression cassette containing the nucleic acid molecule, a recombinant vector, a recombinant bacterium or a transgenic cell line; the DHT1 protein is the protein shown in SEQ ID No. Substitution and / or deletion and / or addition of amino acid residues, or a protein whose sequence has more than 99%, more than 95%, more than 90%, more than 85% or more than 80% homology and has the same function, or its N A fusion protein obtained after tagging at the end and / or C-terminus. Experiments have proved that DHT1 and its coding gene can regulate the plant type of the plant, which has important application value for effectively regulating the plant type by using the gene resource through genetic breeding and genetic engineering methods.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein related to plant type and its coding gene and application. Background technique [0002] Rice is an important food crop that feeds more than one-third of the world's population. With population growth, reduction of arable land and environmental degradation, rice production is facing increasing pressure to ensure yield. The plant type of rice is an important agronomic trait related to yield. The plant type mainly depends on several aspects such as plant height, leaf type, tiller number, tiller angle and panicle shape. Therefore, it is of great significance for rice breeding and production to discover and utilize related genes that control rice plant type. [0003] So far, most of the plant height genes that have been cloned in rice are related to the metabolism or signal transduction of hormones such as gibberellin, brassinosteroids and strigolactone, such as the semi-dwar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C07K14/415A01H5/00A01H5/10A01H6/46
CPCC12N15/8261C07K14/415
Inventor 万建民刘天珍张欣林启冰程治军任玉龙朱杉杉蕾财林郭秀平王洁赵志超
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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