Protein related to plant type as well as coding gene and application of the protein
A protein and plant technology, applied in the field of protein and its coding gene and application, can solve the problem of unknowable regulation of plant type
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[0080] Example 1. Plant type-related protein DHT1 can regulate rice plant height, panicle length and grain width
[0081] This embodiment provides a plant type-related protein derived from rice Kitaake (Oryza sativa var. Kitaake), its name is DHT1, the amino acid sequence of DHT1 in rice Kitaake is SEQ ID No.1, the coding sequence of DHT1 gene ( That is, the CDS sequence) is SEQ ID No.2, and the genome sequence of the DHT1 gene is SEQ ID No.3.
[0082] 1. Construction of an RNA interference vector that reduces the expression of DHT1 gene
[0083] 1. Obtaining of DHT1 gene expression interference fragments
[0084] (1) Total RNA of 14-day seedlings of rice Kitaake (Oryzasativa) was extracted using RNApreppure Plant Total RNA Extraction Kit (Tiangen Biochemical Technology (Beijing) Co., Ltd.), and cDNA was obtained by reverse transcription.
[0085] (2) With the cDNA that step (1) obtains as template, carry out PCR amplification with the primer that DHT1-sense-F and DHT1-sense...
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