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Protein related to plant type as well as coding gene and application of the protein

A protein and plant technology, applied in the field of protein and its coding gene and application, can solve the problem of unknowable regulation of plant type

Active Publication Date: 2021-06-18
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it has been found that such proteins in plants are involved in the regulation of multiple physiological and biochemical processes such as seed germination, photosynthesis, various stress responses, flowering, hormone signal transduction, etc., but the regulation of plant type is still unknown

Method used

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  • Protein related to plant type as well as coding gene and application of the protein
  • Protein related to plant type as well as coding gene and application of the protein

Examples

Experimental program
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Effect test

Embodiment 1

[0080] Example 1. Plant type-related protein DHT1 can regulate rice plant height, panicle length and grain width

[0081] This embodiment provides a plant type-related protein derived from rice Kitaake (Oryza sativa var. Kitaake), its name is DHT1, the amino acid sequence of DHT1 in rice Kitaake is SEQ ID No.1, the coding sequence of DHT1 gene ( That is, the CDS sequence) is SEQ ID No.2, and the genome sequence of the DHT1 gene is SEQ ID No.3.

[0082] 1. Construction of an RNA interference vector that reduces the expression of DHT1 gene

[0083] 1. Obtaining of DHT1 gene expression interference fragments

[0084] (1) Total RNA of 14-day seedlings of rice Kitaake (Oryzasativa) was extracted using RNApreppure Plant Total RNA Extraction Kit (Tiangen Biochemical Technology (Beijing) Co., Ltd.), and cDNA was obtained by reverse transcription.

[0085] (2) With the cDNA that step (1) obtains as template, carry out PCR amplification with the primer that DHT1-sense-F and DHT1-sense...

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Abstract

The invention discloses a protein related to a plant type as well as a coding gene and application of the protein. The invention provides application of DHT1 protein or related biological materials thereof in regulation and control of plant grain development. The related biological materials are nucleic acid molecules capable of expressing the DHT1 protein or expression cassettes, recombinant vectors, recombinant bacteria or transgenic cell lines containing the nucleic acid molecules; and the DHT1 protein is a protein as shown in SEQ ID No.1 or a protein obtained after the protein as shown in SEQ ID No.1 is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, or a protein which has more than 99%, more than 95%, more than 90%, more than 85% or more than 80% of homology in sequence and has the same function, or a fusion protein which is obtained after the N end and / or C end of the protein are / is connected with a label. Experiments prove that the DHT1 and the coding gene thereof can regulate and control the plant type of the plant, and have important application value for effectively regulating and controlling the plant type by utilizing the gene resource with genetic breeding and genetic engineering methods.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein related to plant type and its coding gene and application. Background technique [0002] Rice is an important food crop that feeds more than one-third of the world's population. With population growth, reduction of arable land and environmental degradation, rice production is facing increasing pressure to ensure yield. The plant type of rice is an important agronomic trait related to yield. The plant type mainly depends on several aspects such as plant height, leaf type, tiller number, tiller angle and panicle shape. Therefore, it is of great significance for rice breeding and production to discover and utilize related genes that control rice plant type. [0003] So far, most of the plant height genes that have been cloned in rice are related to the metabolism or signal transduction of hormones such as gibberellin, brassinosteroids and strigolactone, such as the semi-dwar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C07K14/415A01H5/00A01H5/10A01H6/46
CPCC12N15/8261C07K14/415
Inventor 万建民刘天珍张欣林启冰程治军任玉龙朱杉杉蕾财林郭秀平王洁赵志超
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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