A kind of rice grain shape related protein gif1 and its coding gene and application

A technology of related proteins and coding genes, applied in rice grain shape-related protein GIF1 and its coding gene and application field, can solve the problems of unclear and incomplete regulation mechanism of grain shape in rice, complex QTL cloning of quantitative traits, etc.

Inactive Publication Date: 2019-03-01
SICHUAN AGRI UNIV
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  • Application Information

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Problems solved by technology

[0004] In recent years, under the active efforts of scientists all over the world, especially Chinese scientists, the cloning of genes related to rice grain shape and yield traits has made great progress. However, due to the complexity of quantitative trait QTL cloning, the cloned rice grain shape There are still very few genes related to yield traits, and the mechanism of grain shape regulation in rice is still unclear and incomplete

Method used

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  • A kind of rice grain shape related protein gif1 and its coding gene and application
  • A kind of rice grain shape related protein gif1 and its coding gene and application
  • A kind of rice grain shape related protein gif1 and its coding gene and application

Examples

Experimental program
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Effect test

Embodiment 1GI

[0058] Embodiment 1 GIF1 gene rice transgenic test

[0059] 1. Construction of GIF1 gene overexpression vector

[0060] In this example, the expression vector PHB (Mao et al., 2005, PNAS 102: 12270-12275, commercially available) was used as the rice transgene carrier. The vector encodes a bacterial origin of replication (ori), a kanamycin resistance gene (Kan r ), hygromycin resistance gene (Hyg r ), herbicide resistance gene (Bar r ), 2×35S promoter, NOS gene termination signal sequence and multiple cloning site (MCS) for connecting target fragments. The GIF1 gene or its fragment can be inserted into the restriction endonuclease site to construct a transgenic plasmid.

[0061] Using the normal wild-type Nipponbare RNA as a template, the first-strand cDNA was synthesized, and the oligonucleotides at the 5' and 3' ends of the GIF1 gene ORF sequence were used as PCR primers (SEQ ID NO:3 and SEQ ID NO:4), Amplify with high-fidelity enzyme, obtain the full-length cDNA (SEQ ID...

Embodiment 2

[0070] Example 2 GIF1 gene function analysis

[0071] The method described in embodiment 1 obtains the transgenic plant of rice GIF1 overexpression, is divided into 5 groups, named respectively OE1-1, OE1-6, OE2-5, OE4-1, OE4-5, and normal control group control , Observing rice grain phenotypes with transgenic T1 generation plants. The result is as Figure 2-6 as shown, image 3 middle bar=1cm, Figure 3-6 Among them, ** is significant at the 0.01 level. It can be seen from the figure that compared with the control group, the grain length, grain width and thousand-grain weight of the plants OE1-1, OE1-6, OE2-5, OE4-1, and OE4-5 transfected with GIF1 overexpression genes were higher than those of the control group. There was a significant increase. It indicated that the overexpression of GIF1 would increase the grain length and grain width, and then lead to the increase of 1000-grain weight.

[0072] From the above analysis, it can be seen that GIF1 can control rice grain...

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Abstract

The invention provides rice grain shape associated protein GIFI as well as an encoding gene and an application thereof. The GIFI protein has an amino acid sequence shown in SEQ ID NO.2 or an amino acid sequence which has the same function and is formed by replacement, deficiency or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.2; the encoding gene has a nucleotide sequence shown in SEQ ID NO.1 or a nucleotide sequence hybridized with the nucleotide sequence shown in SEQ ID NO.1 under the strict condition. After the GIFI encoding gene is recombined in an expression carrier and transforms rice, overexpressed GIFI can increase the width and length of rice grains and increase the rice yield.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a protein GIF1 related to rice grain shape, its coding gene and its application. Background technique [0002] As an important food crop, rice plays an important role in my country's food security, and continuously increasing the yield of rice per unit area is a major issue related to the national economy and people's livelihood. Grain type has a huge impact on endosperm structure, appearance of rice grains, thousand-grain weight, seed setting rate, and number of grains per panicle. It is not only an important indicator of rice quality, but also a key factor for increasing yield. With the continuous development of gene mapping groups and the further development of new molecular markers, more rice key trait genes have been mapped and cloned. Research on these new genes has greatly enhanced people's understanding of the genetic characteristics and mol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10A01H6/46
CPCC07K14/415C12N15/8261
Inventor 李双成李平高烽焱王世全邓其明郑爱萍朱军刘怀年王玲霞
Owner SICHUAN AGRI UNIV
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