A major qtl locus controlling wheat grain length and closely linked kasp primers and applications

A technology for wheat kernels and wheat, which is applied to biochemical equipment and methods, recombinant DNA technology, and microbial assay/inspection, etc., can solve problems such as low breeding efficiency, increased breeding costs, and time-consuming, saving breeding costs, The effect of improving grain weight and expanding stability

Active Publication Date: 2022-05-20
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the molecular mechanism of inheritance of these traits is not well understood, and it takes a lot of time and effort to identify the phenotype, which not only leads to increased breeding costs, but also low breeding efficiency

Method used

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  • A major qtl locus controlling wheat grain length and closely linked kasp primers and applications
  • A major qtl locus controlling wheat grain length and closely linked kasp primers and applications
  • A major qtl locus controlling wheat grain length and closely linked kasp primers and applications

Examples

Experimental program
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Effect test

experiment example 1

[0023] Experimental example 1 Controlling the acquisition of grain length main effect QTL QKl.sau-6A and KASP-AX-109894590 molecular marker

[0024] (1) F 1 Generation, F 1 F 2 A high-generation self-bred genetic population containing 371 lines was obtained through the single-seed method;

[0025] (2) Using the 55K SNP gene chip to construct Chuannong 18×T1208 containing 371 lines (F 11 -F 13 ) of the recombined inbred population to scan the genome and construct a linkage genetic map, which covers 21 chromosomes of wheat with a total length of 4192.62cM;

[0026](3) During 2016-2017, 2018-2019, and 2019-2020, it was planted at the Qionglai Wheat Breeding Base of Sichuan Agricultural University (Jianjin Town, Qionglai City, Sichuan Province, 30°25′N, 103°28′E, 493.3m above sea level). Random block design is adopted, the plot length is 2m, each row has 10 plants, and the row spacing is 0.25m. Each strain is planted in 4 rows and repeated three times. The cultivation managem...

experiment example 3

[0038] Experimental example 3 Application of KASP-AX-109894590 molecular marker in Chuannong 18×T1208 high-generation self-bred population

[0039] Using the same method as above, randomly select 127 lines (including two parents) in the Chuannong 18×T1208 high-generation self-bred population to extract DNA for fluorescent quantitative PCR genotyping, and the typing results have the same fluorescent signal as Chuannong 18 The ones with the same fluorescent signal as T1208 are recorded as the B genotype. Finally, it can be seen that there are 33 strains with the A genotype and 94 strains with the B genotype, and the strains with the B genotype The grain length of the genotype strains was significantly higher than that of the A genotype strains, and the results are shown in Table 2 and Table 3.

[0040] Table 2 shows the genotyping of 127 lines in Chuannong 18×T1208 high-generation self-bred population

[0041]

[0042]

[0043]

[0044]

[0045]

[0046] Note: A:...

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Abstract

The present invention discloses a major QTL site for controlling wheat grain length and a closely linked KASP primer and application. The genetic position of the major QTL site QK1.sau-6A on the 6A chromosome of wheat is 16.78-31.64 cM, the SNP molecular markers on both sides of the major QTL site QK1.sau-6A are AX-110126107 and AX-109894590, respectively, and their physical positions on the Chinese Spring reference genome IWGSC RefSeq v1.0 are 13.35Mb and 617.82Mb, respectively . The present invention discloses for the first time a main QTL site QK1.sau-6A that controls grain length. This QTL can significantly increase grain length and grain weight of wheat, and has high value for high-yield wheat breeding.

Description

technical field [0001] The invention relates to a main effect QTL locus for controlling wheat grain length, in particular to a main effect QTL locus for controlling wheat grain length, closely linked KASP primers and application thereof. Background technique [0002] Wheat is one of the three major staple crops in the world, providing about 20% of protein and calories in human food. In recent years, with the reduction of arable land and the influence of population growth and many other factors, it is difficult for future food production to meet people's demand for food. It is predicted that by 2050, the annual growth rate of grain production needs to be at least 2.4% to meet people's demand for food. The yield of wheat is mainly affected by three factors of yield, namely the number of spikes per unit area, thousand-grain weight, and the number of grains per spike. Thousand-grain weight, one of the three factors of yield, is mainly affected by grain traits, including grain ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113C12Q2563/107
Inventor 任天恒范涛李治欧霞谭飞泉
Owner SICHUAN AGRI UNIV
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