A major qtl locus controlling wheat grain length and closely linked kasp primers and applications
A technology for wheat kernels and wheat, which is applied to biochemical equipment and methods, recombinant DNA technology, and microbial assay/inspection, etc., can solve problems such as low breeding efficiency, increased breeding costs, and time-consuming, saving breeding costs, The effect of improving grain weight and expanding stability
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experiment example 1
[0023] Experimental example 1 Controlling the acquisition of grain length main effect QTL QKl.sau-6A and KASP-AX-109894590 molecular marker
[0024] (1) F 1 Generation, F 1 F 2 A high-generation self-bred genetic population containing 371 lines was obtained through the single-seed method;
[0025] (2) Using the 55K SNP gene chip to construct Chuannong 18×T1208 containing 371 lines (F 11 -F 13 ) of the recombined inbred population to scan the genome and construct a linkage genetic map, which covers 21 chromosomes of wheat with a total length of 4192.62cM;
[0026](3) During 2016-2017, 2018-2019, and 2019-2020, it was planted at the Qionglai Wheat Breeding Base of Sichuan Agricultural University (Jianjin Town, Qionglai City, Sichuan Province, 30°25′N, 103°28′E, 493.3m above sea level). Random block design is adopted, the plot length is 2m, each row has 10 plants, and the row spacing is 0.25m. Each strain is planted in 4 rows and repeated three times. The cultivation managem...
experiment example 3
[0038] Experimental example 3 Application of KASP-AX-109894590 molecular marker in Chuannong 18×T1208 high-generation self-bred population
[0039] Using the same method as above, randomly select 127 lines (including two parents) in the Chuannong 18×T1208 high-generation self-bred population to extract DNA for fluorescent quantitative PCR genotyping, and the typing results have the same fluorescent signal as Chuannong 18 The ones with the same fluorescent signal as T1208 are recorded as the B genotype. Finally, it can be seen that there are 33 strains with the A genotype and 94 strains with the B genotype, and the strains with the B genotype The grain length of the genotype strains was significantly higher than that of the A genotype strains, and the results are shown in Table 2 and Table 3.
[0040] Table 2 shows the genotyping of 127 lines in Chuannong 18×T1208 high-generation self-bred population
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[0046] Note: A:...
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