Single nucleotide polymorphism (SNP) molecular marker linked with maximum root length QTL QMrl.sau-3D of wheat and application of SNP molecular marker

A molecular marker, wheat technology, applied in the field of molecular genetic breeding of wheat, can solve the problem of not many molecular marker-assisted breeding and so on

Active Publication Date: 2022-07-29
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006]In recent years, many QTLs related to maximum root length have been reported on different chromosomes of wheat, but few have been applied to actual molecular marker-assisted breeding

Method used

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  • Single nucleotide polymorphism (SNP) molecular marker linked with maximum root length QTL QMrl.sau-3D of wheat and application of SNP molecular marker
  • Single nucleotide polymorphism (SNP) molecular marker linked with maximum root length QTL QMrl.sau-3D of wheat and application of SNP molecular marker
  • Single nucleotide polymorphism (SNP) molecular marker linked with maximum root length QTL QMrl.sau-3D of wheat and application of SNP molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Acquisition of QTL QMrl.sau-3D for maximum root length in wheat and its molecular marker KASP-Mrl-sau1

[0039] (1) Using the wheat line '20828' as the female parent and the wheat line 'SY95-71' as the male parent, a recombinant inbred line containing 128 lines was obtained by single-seed transmission, forming a genetic mapping population.

[0040] (2) Identification of the maximum root length phenotype of the recombinant inbred line population: using seeds from the recombinant inbred line population from 5 different environments (Ya'an in 2017, Ya'an in 2018, Chongzhou in 2018, Chongzhou in 2019 and Wenjiang in 2019) As the test material, a hydroponic test at the seedling stage was carried out. Each line was measured for the maximum root length of 9 seedlings, and the average value was obtained to represent the root length of the line, and the BLUP value in different environments was calculated. .

[0041] (3) 55K SNP chip analysis

[0042] a) DNA extractio...

Embodiment 2

[0059] Example 2 Application of molecular marker KASP-Mrl-sau1 in selecting QTL QMrl.sau-3D for controlling maximum root length

[0060] (1) A recombinant inbred line was constructed using the common wheat line 'S849-8' with a shorter maximum root length as the female parent and the common wheat line with a longer maximum root length 'SY95-71' as the male parent. -8' and 'SY95-71' for KASP-Mrl-sau1 labeling detection.

[0061] (2) 86 lines were randomly selected from the progeny lines of the recombinant inbred lines, and the KASP-Mrl-sau1 marker was detected to verify the practicability of KASP-Mrl-sau1. The specific method is as follows: extract the DNA of 'S849-8' and 'SY95-71' and 86 strains; use it as a template, use the specific primer pair of KASP-Mrl-sau1 to carry out quantitative PCR amplification, and carry out fluorescence quantitative PCR amplification The read value, the specific primer pair is:

[0062]Primer on FAM tag: (the underlined part is the FAM tag seque...

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Abstract

The invention discloses a wheat maximum root length QTLQMrl.sau-3D linked SNP (Single Nucleotide Polymorphism) molecular marker and application thereof, and relates to the field of wheat molecular genetic breeding. The SNP molecular marker and QMrl.sau-3D are co-localized on a 3D chromosome long arm of a wheat genome, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.16, and C/T mutation exists at the 36bp position of the SNP molecular marker. The SNP molecular marker KASP-Mrl-sau1 is closely linked with the maximum root length QTLQMrl.sau-3D of wheat, the maximum root length QTLQMrl.sau-3D of wheat can be accurately tracked, the maximum root length characteristic of wheat can be predicted, the selection and identification efficiency of the maximum root length of wheat can be remarkably improved, wheat varieties or strains with the long root length can be rapidly screened out, and the application prospect is wide. The process of wheat molecular genetic breeding is accelerated.

Description

technical field [0001] The invention relates to the field of wheat molecular genetics and breeding, in particular to a QTL QMrl.sau-3D linked SNP molecular marker for the maximum root length of wheat and its application. Background technique [0002] Common wheat (Triticum aestivum L.), one of the first grains to be domesticated, is listed as a "big three" cereal crop worldwide due to its better environmental adaptability, richer nutrition and higher yield potential Widely planted. Due to population growth and continuous reduction of arable land, wheat yield needs to be increased urgently, and genetic improvement of wheat is one of the main means to increase yield. [0003] The root system is one of the most important organs of plants and plays a key role in regulating the absorption of water and nutrients by plants, as well as the transformation and transportation of substances in plants. In the study of root-related traits, the contribution of the maximum root length to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6851C12N15/11
CPCC12Q1/6895C12Q1/6851C12Q2600/156C12Q2600/13C12Q2531/113C12Q2563/107
Inventor 马建陈黄鑫周界光玄其京曾照勇唐华苹江千涛蒋云峰魏育明郑有良兰秀锦
Owner SICHUAN AGRI UNIV
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