Single nucleotide polymorphism (SNP) molecular marker linked with maximum root length QTL QMrl.sau-3D of wheat and application of SNP molecular marker
A molecular marker, wheat technology, applied in the field of molecular genetic breeding of wheat, can solve the problem of not many molecular marker-assisted breeding and so on
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Embodiment 1
[0038] Example 1 Acquisition of QTL QMrl.sau-3D for maximum root length in wheat and its molecular marker KASP-Mrl-sau1
[0039] (1) Using the wheat line '20828' as the female parent and the wheat line 'SY95-71' as the male parent, a recombinant inbred line containing 128 lines was obtained by single-seed transmission, forming a genetic mapping population.
[0040] (2) Identification of the maximum root length phenotype of the recombinant inbred line population: using seeds from the recombinant inbred line population from 5 different environments (Ya'an in 2017, Ya'an in 2018, Chongzhou in 2018, Chongzhou in 2019 and Wenjiang in 2019) As the test material, a hydroponic test at the seedling stage was carried out. Each line was measured for the maximum root length of 9 seedlings, and the average value was obtained to represent the root length of the line, and the BLUP value in different environments was calculated. .
[0041] (3) 55K SNP chip analysis
[0042] a) DNA extractio...
Embodiment 2
[0059] Example 2 Application of molecular marker KASP-Mrl-sau1 in selecting QTL QMrl.sau-3D for controlling maximum root length
[0060] (1) A recombinant inbred line was constructed using the common wheat line 'S849-8' with a shorter maximum root length as the female parent and the common wheat line with a longer maximum root length 'SY95-71' as the male parent. -8' and 'SY95-71' for KASP-Mrl-sau1 labeling detection.
[0061] (2) 86 lines were randomly selected from the progeny lines of the recombinant inbred lines, and the KASP-Mrl-sau1 marker was detected to verify the practicability of KASP-Mrl-sau1. The specific method is as follows: extract the DNA of 'S849-8' and 'SY95-71' and 86 strains; use it as a template, use the specific primer pair of KASP-Mrl-sau1 to carry out quantitative PCR amplification, and carry out fluorescence quantitative PCR amplification The read value, the specific primer pair is:
[0062]Primer on FAM tag: (the underlined part is the FAM tag seque...
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