50results about How to "Easy identification" patented technology

The invention discloses a molecular marking method of an indica rice variety subjected to anti-brown planthopper host gene Bph27 transfer. The developed and designed gene Bph27 is closely interlocked with molecular marking primers B471 and B58, and differential fragments can be amplified from an anti-brown planthopper donor Balamawee and a susceptible indica rice variety; the anti-brown planthopper host gene Bph27 is genetically transferred through molecular marking of a gene Bph27 locus, the required fragments can be accurately and quickly guided, the selection efficiency of the gene Bph27 is greatly improved, and a new anti-brown planthopper indica rice variety 991RB is developed.

The invention provides an SNP marker related to the sugar tolerance of grass carps and application of the SNP marker. The SNP marker comprises two SNP loci, namely H3 and H4, wherein H3 is located at position 1817 from the 5' end of the nucleotide sequence shown as SEQ ID NO:1 in the specification, and base at the position 1817 is T or C; H4 is located at position 1818 from the 5' end of the nucleotide sequence shown as SEQ ID NO:1 in the specification, and base at the position 1818 is G or T. The SNP marker provided by the invention is closely related to the sugar tolerance of the grass carps, and can be effectively used for identification or selective breeding of high-sugar-tolerance grass carp species.

The invention discloses a primer and kit for the real-time fluorescence quantification PCR detection of a wild strain TaqMan-MGB of a cattle nodular skin disease virus and a detection method. According to the method, two primers and a specific MGB probe are designed and respectively have the DNA sequences of SEQ ID NOs.1-3. The kit contains amplification reaction liquid, a positive control, a negative control and sterilized deionized water. A target sequence can be rapidly, efficiently, specifically and sensitively detected by virtue of a two-step amplification method, the operation is simpleand convenient, expensive instruments and reagents are not used, the technical requirements on operators are not required, the detection cost is low, the flux is high, and the detection period is short.

The invention relates to the technical field of virus nucleic acid detection methods, and particularly relates to an RT-LAMP nucleic acid detection primer group of SARS-CoV-2 and a closed SARS-CoV-2 isothermal amplification nucleic acid detection kit. According to the kit provided by the invention, nucleic acid extraction is not needed for virus RNA of a throat swab sample, and the sample can be directly amplified. The kit is a visual instant screening kit for the SARS-CoV-2 by a one-step method. The kit has good specificity, can eliminate non-specific interference of other coronavirus strains, is quick and efficient, and can complete detection within one hour. Large expensive and precise instruments and equipment are not needed, only a common metal bath or water bath kettle is needed, detection cost is low, toxic reagents are not involved and closed operation is performed in the whole process, secondary pollution is avoided, and safety of operators and an environment is ensured.

The invention discloses a miR-3197 molecular marker for detecting type 2 diabetic retinopathy and an amplification primer and an application thereof. The miR-3197 molecular marker is characterized in that a nucleotide sequence of the miR-3197 molecular marker is 5'GGAGGCGCAGGCUCGGAAAGGCG-3', and a nucleotide sequence of a positive amplification primer of the miR-3197 molecular marker is 5'-GGA GGC GCA GGC TCG GAA -3'. The miR-3197 molecular marker has the advantages of high detection efficiency and high specificity, and can be applied to auxiliary diagnosis, detection or medicament screening of the type 2 diabetic retinopathy.

The invention belongs to the field of molecular biology DNA labeling technology and application, and in particular relates to Apostichopus japonicas express sequence tag EST micro-satellite label screening development and application. The invention provides Apostichopus japonicas AjE101 micro-satellite specific DNA primers, a polymerase chain reaction (PCR) reaction system by utilizing the primers, and a detection method for an Apostichopus japonicas AjE101 micro-satellite DNA label. The detection method comprises the following steps of: extracting genome of Litopenaeus vannamei Boone; designing the specific primers at two ends of an Apostichopus japonicas AjE101 micro-satellite DNA core sequence; and performing PCR amplification on genome DNA of different groups of the Litopenaeus vannamei Boone or individuals in the group by using the primers, analyzing products, and determining the genome of each individual so as to obtain an Apostichopus japonicas polymorphism genetic variation map. The invention is mainly applied to Apostichopus japonicas germ plasm resource and genetic diversity analysis, cular population genetics, construction of genetic maps and research of functional genes.

The invention relates to a LAMP reagent kit used for detecting O type foot-and-mouth disease virus, and an establishing method and the application thereof. The reagent kit is a detection system composed of LAMP reaction solution including six LAMP primers; the LAMP reaction solution of 23 microlitres has the concrete collocations just as the rightward presentation; the detection system can detect the O type foot-and-mouth disease virus rapidly, conveniently and efficiently under the isothermal condition in a high specific and high sensitive manner without complicated instruments; therefore, a new technology platform is provided for food safety inspection, and the urgent need for the field detection on the foot-and-mouth disease at present is better satisfied; the LAMP reagent is used in the field detection of import and export quarantines, food sanitation departments, animal husbandry farms, etc., is easy for large-scale popularization and application, and has wide market prospect, and bigger economic benefits as well as social benefits.

Sequences of primers for identifying fusarium solani and / or fusarium oxysporum, a kit and a molecular detection method thereof are disclosed. By verification, the primer sequences, the kit and the method have high specificity. By utilization of the group of the primers and the method, the fusarium solani and the fusarium oxysporum can be identified rapidly and accurately. Establishment of a system has important meaning for rapid early-phase detection of melon root rot and wilt, soil germ distribution and disease control. In addition, establishment of a molecular detection system based in TEF1-alpha as a target gene provides theoretical foundations and identification methods for complex systematic classification and identification of fusarium pathogenic bacteria.

The invention discloses a polymerase chain reaction (PCR) detection method for a trans-cry2A-genic rice line T2A-1 and application thereof, which relate to the technical field of biology. The PCR detection method for the trans-cry2A-genic rice line T2A-1 is characterized in that: a forward primer in PCR is designed according to base sequences from the first site to the 473rd site of a nucleotide sequence shown by SEQ ID NO.1, while a reverse primer is designed according to the base sequences of the 474th to 708th sites of the nucleotide sequence shown by SEQ ID NO.1. The method has the advantage of fast and accurately identifying the trans-cry2A-genic rice line T2A-1 and rice lines taking the trans-cry2A-genic rice line T2A-1 as a parent by utilizing an ordinary PCR apparatus and a reagent.

The invention discloses a primer composition for identification of human adenovirus type 55 and application thereof. The primer composition provided by the invention includes a DNA fragment-I, II, a DNA fragment-II, a DNA fragment-III and a DNA fragment-IV, all the fragments are single-stranded DNA fragments and are shown as sequence 1, sequence 2, sequence 3 and sequence 4 in order. The primer composition can also include a single-stranded DNA fragment-V and / or a single-stranded DNA fragment-VI, and the fragments are shown as sequence 5 and sequence 6 in order. The primer composition provided by the invention is applicable to specific detection and quantitative analysis on the human adenovirus type 55. The primer composition provided by the invention has the advantages of: (1) amplification reaction just under constant temperature and no need for special equipment; (2) high specificity; (3) fast speed and high efficiency, completion of the amplification reaction in a short period of time; (4) high sensitivity; and (5) convenient and simple identification. The primer composition provided by the invention is especially suitable for rapid detection of viruses in clinical samples.

The present invention relates to a cosmetic container which is coupled to an airless outlet pump and is capable of discharging contents by a constant amount. More specifically, a transparent outer container is coupled to an opaque inner container which is dually coupled to the airless outlet pump, wherein an insertion groove is formed on the inner surface of the outer container, and a remaining amount indicator is connected to a disc inside of the inner container and inserted into the insertion groove, so that by this configuration and the opaque inner container, denaturation and discoloration and the like can be prevented; and various printing portions are formed on the outer surface of the inner container, thereby providing product features and producing a uniqueness for a product; and since the printing portions of the inner container is seen through the transparent outer container a more luxurious aesthetic is produced; and convenience of use is further improved since the remaining amount of the contents can be precisely and easily recognized and checked as the remaining amount indicator can be seen with the naked eye through the outer container.

The invention provides a gene sequence for identifying the sex of tapisicia sinensis. The gene sequence is used for identifying the male and amphiprotic sex of tapisicia sinensis, and through the SCAR molecular marker primer acquired by utilizing the gene sequence, the male and amphiprotic sex of tapisicia sinensis can be rapidly and simply judged. The SCAR marker primer for identifying the sex of tapisicia sinensis comprises a forward primer TS-SCAR-F and a reverse primer TS-SCAR-R, and the specific sequences are TS-SCAR-F:5'-ATCTTCATAGCCGCATAGCAAA-3', and TS-SCAR-F:5'-CCAAATCCTTCAACAATATACCTG-3'. The acquired male-specific SRAP molecular marker is successfully converted into a stable, accurate and simple SCAR marker which can be used for completely identifying different sexes of tapisicia sinensis. The SCAR primer combination can be used for accurately identifying sexes of manually cultured tapisicia sinensis and tapisicia sinensis at different regions of wild populations, and the application range is wide.

The invention discloses a pet socializing system and method. The method comprises the steps of receiving the first pet file information of a first user; determining the first type and the first gradeof a first pet; determining the first price of the first pet based on the first type and the first grade; displaying the first price, the first type and the first grade of the first pet so as to recommend a second pet matching with the first pet. The invention is advantageous in that personal buyer and seller can conduct on-line pet identification, off-line pet trading and pairing, and can get ridof the neglect of pet grade of a pet store, and make profit; it is convenient for a pet hospital to identify the pet grade, customize price and carry out a more comprehensive counting.

The present invention relates to a millet waxy gene cosegregation molecular marker and a detection method thereof, which are used for utilization of millet waxy resource and breeding of a novel waxy variety. The molecular marker is an InDel molecular marker, and an amplification primer is a waxy-TSI2 / int1 primer combination and a waxy-int1-1F / 4R primer combination.

The invention belongs to the technical field of microorganisms, and particularly relates to streptomyces venezuelae, and an authentication method and application thereof. The invention screens an oiland gas indication microorganism, i.e., the streptomyces venezuelae, with high specificity, the streptomyces venezuelae in soil can be quickly detected through a specific primer, a defect that an authentication period is short and an applicable range is small in the common art is eliminated so as to be quick and convenient, and the concentration anomaly characteristics of the microorganism are analyzed to predict an oil and gas enrichment region and an oil and gas reservoir. The invention also provides a method for indicating the concentration of upward floating gaseous hydrocarbon of the oiland gas reservoir by the streptomyces venezuelae. The method adopts an improved primer to amplify the 16S rDNA conserved sequence of the streptomyces venezuelae, and according to the luminance of a target stripe in an amplification product, the concentration of the floating gaseous hydrocarbon can be accurately and efficiently judged.

The invention provides an immunomagnetic head negative enrichment method and an application thereof. The method comprises the following steps: obtaining a separating medium from a pretreated pleuroperitoneal fluid sample, balancing, and centrifuging to remove deposited red blood cells; performing adsorption reaction on supernatant liquid and magnetic beads; centrifuging, balancing all liquid deposited on the magnetic beads by using a hCTC buffer solution, and then placing on a magnetic frame to adsorb the magnetic beads; centrifuging, then collecting supernatant liquid, and balancing the supernatant liquid by using the hCTC buffer solution; centrifuging to remove the supernatant; and counting cells, diluting, smearing and drying, and then performing FISH (Fluorescence In Situ Hybridization) detection. By combining the FISH detection, the method provided by the invention can improve the sensitivity and specificity of tumor cell enrichment, and is used for differential diagnosis of benign and malignant pleuroperitoneal fluid, dynamic gene detection of solid tumors, thereby realizing individualized treatment.

The invention belongs to the field of breeding of plants and discloses a molecular marker MAG7237 of a wheat fusarium head blight infection resistant gene Fhb4 and application of the molecular marker MAG7237. The genetic distance between the molecular marker MAG7237 and the Fhb4 gene is 0.34 cm. The molecular marker most closely linked with the Fhb4 is acquired by the invention for the first time in the world. The molecular marker is a co-dominant marker with the advantages of being convenient to detect, and stable and simple to amplify; by adopting the marker MAG7237 to detect the Fhb4 gene, the existence of the Fhb4 or not and the existence state can be determined, and the fusarium head blight resistance of the wheat can be forecasted; and furthermore, the plants with the Fhb4 can be fast screened and used for breeding of disease-resistant varieties.

The invention discloses fluorescence quantitative RT-PCR primers and probe and method for detecting APPV (atypical porcine pestivirus) as well as an application. The sequences of the primers and probeare shown in SEQ ID NO:1-3. The APPV can be rapidly, specifically and sensitively detected, and the lowest detection concentration is 5 copies / mu L. The used operation method is simple, a reaction result is easy to judge, the sensitivity and detection rate are high, the false positive phenomenon can be avoided, and the primers and probe are applicable to disease monitoring, scene emergency and detection of clinical samples and are suitable for popularization and application on a large scale.

The invention relates to universal primers capable of highly efficiently and quickly amplifying cephalopod mitochondrial COI genes of various cephalopods, and a design and amplification method thereof. By designing the universal primers of the cephalopod mitochondrial COI genes, various cephalopod COI genes can be amplified at a time in batches, specific primers are not needed to be designed for acertain single species, the time cost is saved, the work efficiency is greatly improved, and the method has high-efficiency and fast characteristics and provides important molecular means and data accumulation for species identification, systematic evolution, germplasm resource conservation and sustainable development and utilization of the cephalopods.

The invention discloses microsatellite primers, a kit and an identification method for identifying tridacna crocea, tridacna squamosa and hybrid first filial generations of the tridacna crocea and thetridacna squamosa. The microsatellite primers TC111 are represented as F: 5'-CCATTTTGGTGCGTAGTA-3' and R: 5'-CTTGAGGGCACCTTTTAG-3'. With the adoption of the method, the tridacna crocea, the tridacnasquamosa and the hybrid first filial generations of the tridacna crocea and the tridacna squamosa can be rapidly and accurately identified, the method has important actual application value, and meanwhile, the method has the advantages of being simple and low in cost, obtaining a direct, accurate and effective result, avoiding influence of the environment and the development stage and the like.

The invention belongs to the technical field of traditional Chinese medicine quality identification, and particularly relates to a shape feature collection device, a database and an identification system for traditional Chinese medicine. The identification unit is used for comparing and identifying at least one shape feature of the traditional Chinese medicine to be identified with a corresponding shape feature in the shape feature database, and outputting a comparison and identification result; and the identification result display unit is used for displaying the comparison identification result of the identification unit. The traditional Chinese medicine identification system is based on a machine vision technology and is combined with an artificial intelligence technology to finally generate a traditional Chinese medicine variety standard image library, so that traditional Chinese medicine morphological characteristic identification standardization is realized, which is an innovative application of intelligence and standardization in traditional Chinese medicine morphological identification, and has great significance in promoting traditional Chinese medicine identification accuracy and improving traditional Chinese medicine decoction piece quality. And the traditional Chinese medicine composition has important effect and significance for ensuring clinical curative effect.

The invention discloses a miRNA (micro ribonucleic acid) molecular marker for detecting type 2 diabetes mellitus, and amplification primers and application of the miRNA molecular marker. The molecularmarker has the characteristics that a nucleotide sequence of an miR-145-5P molecular marker is 5'-GUCCAGUUUUCCCAGGAAUCCCU-3'; a nucleotide sequence of a forward amplification primer of the miR-145-5Pmolecular marker is 5'-GAGGGCACACCGTCGCGA-3'; and the molecular marker can be applied to a kit for detecting the type 2 diabetes mellitus. The molecular marker has the advantages that the molecular marker is high in detection efficiency and strong in pertinence and can be used for auxiliary diagnosis, detection or drug screening of the type 2 diabetes mellitus.

The invention provides a Pleurotus nebrodensis volatile flavor component extract and an extraction method and an identification method of the extract. The extract contains fatty acids, esters and pyrimidines, wherein the weight of fatty acids accounts for 97.96% of the total weight of the extract, the weight of esters accounts for 1.46% of the total weight of the extract, and the weight of pyrimidines accounts for 0.58% of the total weight of the extract. The invention adopts supercritical CO2 extraction technology to extract Pleurotus nebrodensis volatile flavor components, studies the optimum technological parameters systematically and analyzes and identifies the volatile flavor components by adopting optimized chromatography-mass spectrometry analytical method, so that the invention provides a basis and a method for full understanding of composition of Pleurotus nebrodensis and further development and application of Pleurotus nebrodensis.

The invention discloses a visual kit for detecting staphylococcus aureus through loop-mediated isothermal amplification (LAMP) and application thereof. The kit comprises a premixed reaction liquid, a fluorescent color developing agent and a detection tube, wherein sterilized filter paper is attached to an inner cap of the detection tube; the premixed reaction liquid comprises BstDNA (bacillus stearothermophilus deoxyribonucleic acid) polymerase, a reaction buffer, dNTP (deoxy-ribonucleoside triphosphate), magnesium sulfate, a specific primer group, betaine and sterile double distilled water. Staphylococcus aureus in samples can be rapidly detected by using the kit through simple treatment of the samples. The kit has the advantages of simplicity and convenience in operation, rapidness, convenience in result determination, strong specificity, high sensitivity, low cost, and the like.

The invention relates to the technical field of biology, and particularly provides an application of an OPTN gene in identification of dental pulp stem cells for identifying the dental pulp stem cells, and a method thereof and a product. The biomarker OPTN gene capable of being used for identifying the dental pulp stem cells is discovered for the first time through tests, and the high-purity dental pulp stem cells can be identified and separated through differential expression of the gene.

The invention belongs to the technical field of biology, and particularly relates to a method for genotyping by utilizing high-throughput sequencing, a kit and application of the kit. According to theinvention, a set of universal barcode fragments similar to the kit is constructed, so that genotype identification is carried out at a lower cost and a higher speed. According to the invention, a novel genotype identification technology developed by combining barocode and next-generation sequencing is quite efficient and simple for identification of SNP and InDel, the required cost is far lower than the market price, and especially the advantage of identification of large-batch samples is more outstanding. In addition, the technology can also be applied to gene localization, genetic map drawing and the like, so that the application range of the technology is quite wide.

The invention discloses a recycling device, system and method for recyclable waste. The recycling device comprises a ceiling and a plurality of recycling boxes arranged below the ceiling. Each recycling box comprises a box body, a box door and a recycling window provided with a box top, wherein a cover plate is arranged on the recycling window, one side of the cover plate is hinged to the box top,the other side of the cover plate is connected with the box top through an electric lock, the electric lock is in a normally closed state and is unlocked through wireless control, an electronic scaleand an iron basket are arranged in the box body, the electronic scale is arranged at the bottom of the box body, and the iron basket is arranged on the electronic scale. According to the device, theelectric locks of the recycling windows are unlocked in a wireless control mode, and the recycling windows are opened; the waste is weighed, corresponding points can be redeemed according to the weight of the waste, the points can be used for product purchase from a vending machine or a supermarket, and the enthusiasm of people for classified throwing of the waste is mobilized.

The invention discloses a method for detecting bar-transgenic sugarcane and appliance. The method comprises the steps of: directly immersing sugarcane leaves to be detected into herbicide solution and processing; and judging the result by directly observing the damage level, compared with the bar gene-free sugarcane leaves. The method is used for appraising bar gene-containing sugarcane plant, is especially suitable for the fast diction of the transgenosis regeneration plant, and can greatly reduce the detection for the molecule of the transgenosis plant, greatly improve the detection efficiency, and reduce the detection cost.

The invention relates to a dye fluorescent quantitative RT PCR primer, a kit and application thereof. The primer comprises an upstream primer MRV F and a downstream primer MRV R, wherein the nucleotide sequence of the upstream primer MRV F is shown as SEQ ID NO: 1, and the nucleotide sequence of the downstream primer MRV R is shown as SEQ ID NO: 2. The specific primer and the kit can be used forrapidly and accurately identifying the positive reovirus of a mammal, and have the characteristics of simplicity, rapidness, high sensitivity and strong specificity.

The invention discloses a primer group and a detection system for detecting poliovirus by a loop-mediated isothermal amplification technique. The primer group for detecting the poliovirus by the loop-mediated isothermal amplification technique consists of nucleotide sequences shown as SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, and SEQ ID No.4 in a sequence table. The detection system can quickly, conveniently, efficiently and highly specifically and sensitively detect the poliovirus under the isothermal condition without any complex instrument, and provides a novel technical platform for the detection of the poliovirus.