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Molecular marking method of indica rice variety subjected to anti-brown planthopper host gene Bph27 transfer

A molecular marker and brown planthopper resistance technology, applied in the field of molecular genetics, can solve the problems of low efficiency of insect resistance identification and slow progress of insect resistance breeding, etc., and achieve the effect of convenient identification, convenient and fast detection, and clear fragment position

Inactive Publication Date: 2015-03-04
NANJING AGRICULTURAL UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

The variability of BPH biotypes and the complexity of insect resistance identification lead to low efficiency of conventional breeding methods and slow progress in insect resistance breeding

Method used

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  • Molecular marking method of indica rice variety subjected to anti-brown planthopper host gene Bph27 transfer
  • Molecular marking method of indica rice variety subjected to anti-brown planthopper host gene Bph27 transfer
  • Molecular marking method of indica rice variety subjected to anti-brown planthopper host gene Bph27 transfer

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Experimental program
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Effect test

Embodiment Construction

[0022] Materials and Methods:

[0023] (1) Materials and group construction

[0024] F obtained by crossing the brown planthopper-resistant variety Balamawee with 02428 1 , selfed to obtain a F containing 135 individuals 2 Primary segregation population, and each F 2 The corresponding F obtained by selfing of a single plant 2:3 family lineage. The genetic population was further expanded using a F of Balamawee / 02428 containing 18308 individuals 2 Isolate populations for fine mapping of Bph27.

[0025] (2) Phenotypic identification

[0026] The insect source was originally collected from rice fields in the suburbs of Nanjing, and has been raised on Taichung Local No. 1 (TN1) for more than 10 generations. In this study, N. lugens were bred on TN1 to provide the source of insects needed for identification. Breeding in the greenhouse of Nanjing Agricultural University. Pay attention to natural enemies and ants.

[0027] Balamawee, 02428, F1 single plant and 135 F 2:3 Ins...

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Abstract

The invention discloses a molecular marking method of an indica rice variety subjected to anti-brown planthopper host gene Bph27 transfer. The developed and designed gene Bph27 is closely interlocked with molecular marking primers B471 and B58, and differential fragments can be amplified from an anti-brown planthopper donor Balamawee and a susceptible indica rice variety; the anti-brown planthopper host gene Bph27 is genetically transferred through molecular marking of a gene Bph27 locus, the required fragments can be accurately and quickly guided, the selection efficiency of the gene Bph27 is greatly improved, and a new anti-brown planthopper indica rice variety 991RB is developed.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a molecular marker method for transgenic indica rice varieties with main gene Bph27 resistant to brown planthopper. Background technique [0002] Brown planthopper (Nilaparvata lugens ), also known as brown rice louse, is a world-renowned migratory pest and one of the main pests of rice, seriously affecting the yield and quality of rice. In recent years, the annual damage area has reached more than 200 million mu, and since 2005, it has occurred in successive years. In 2005, the damage area of ​​rice planthoppers in my country reached 386 million mu, and the occurrence area in 2006 and 2007 reached 500 million mu. So far, the main pest control measures are the use of chemical pesticides, and the long-term and extensive use of chemical pesticides not only worsens the environment, seriously endangers human health, but also promotes the resistance of pests to chemical pesticides, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 万建民刘裕强陈亮明江玲何俊刘艳玲仇泽宇赵志刚王益华刘喜刘世家田云录
Owner NANJING AGRICULTURAL UNIVERSITY
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