Molecular marking method of indica rice variety subjected to anti-brown planthopper host gene Bph27 transfer

Abstract

The invention discloses a molecular marking method of an indica rice variety subjected to anti-brown planthopper host gene Bph27 transfer. The developed and designed gene Bph27 is closely interlocked with molecular marking primers B471 and B58, and differential fragments can be amplified from an anti-brown planthopper donor Balamawee and a susceptible indica rice variety; the anti-brown planthopper host gene Bph27 is genetically transferred through molecular marking of a gene Bph27 locus, the required fragments can be accurately and quickly guided, the selection efficiency of the gene Bph27 is greatly improved, and a new anti-brown planthopper indica rice variety 991RB is developed.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a molecular marker method for transgenic indica rice varieties with main gene Bph27 resistant to brown planthopper. Background technique [0002] Brown planthopper (Nilaparvata lugens ), also known as brown rice louse, is a world-renowned migratory pest and one of the main pests of rice, seriously affecting the yield and quality of rice. In recent years, the annual damage area has reached more than 200 million mu, and since 2005, it has occurred in successive years. In 2005, the damage area of ​​rice planthoppers in my country reached 386 million mu, and the occurrence area in 2006 and 2007 reached 500 million mu. So far, the main pest control measures are the use of chemical pesticides, and the long-term and extensive use of chemical pesticides not only worsens the environment, seriously endangers human health, but also promotes the resistance of pests to chemical pesticides, ...

AI Technical Summary

Problems solved by technology

The variability of BPH biotypes and the complexity of insect resistance identification lead to

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