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Molecular marking method for indica-japonica segment substitution of fertility loci of indica-japonica hybrid

A technology of molecular markers and sites, applied in the field of molecular genetics, can solve the problems of hybrid sterility between indica and japonica subspecies, and achieve the effects of convenient identification, cost saving and production cost saving

Active Publication Date: 2012-09-12
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

This method is suitable for the breeding of indica-japonica offspring fertility locus segment replacement lines, which can solve the problem of hybrid sterility between indica-japonica subspecies and promote the utilization of heterosis among indica-japonica subspecies

Method used

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  • Molecular marking method for indica-japonica segment substitution of fertility loci of indica-japonica hybrid
  • Molecular marking method for indica-japonica segment substitution of fertility loci of indica-japonica hybrid
  • Molecular marking method for indica-japonica segment substitution of fertility loci of indica-japonica hybrid

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Embodiment Construction

[0052] (1) On the one hand, the CMS line Samsara 422S was crossed with the japonica rice variety Zhendao 88 to obtain the hybrid F 1 , to hybrid F 1 As the female parent, Zhendao 88 was used as the recurrent parent to obtain the backcross population BC 1 f 1 , from BC 1 f 1 At the seedling stage, the markers RMz5 at the S5 site and RMz8 at the S8 site were used for detection. Samsara 422S is a wide-compatibility variety. When amplified with molecular marker RMz5, a fragment with a size of 105 bp can be obtained, and when amplified with molecular marker RMz8, a fragment with a size of 131 bp can be obtained (see Table 2). Zhendao 88 is a japonica rice variety. When amplified with molecular marker RMz5, a 131bp fragment can be obtained; when amplified with molecular marker RMz8, a 145bp fragment can be obtained. Lines with the desired indica segment detected at the fertility locus are used as the female parent to cross the background parent at the flowering stage, and the s...

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Abstract

The invention provides a molecular marking method for indica-japonica fragment substitution of fertility loci of an indica-japonica hybrid, which belongs to the field of molecular genetics. Molecular marker primers RMz 5, RMz7, RMz8 and RMz9 which are developed and designed by the invention amplify differential fragments at infertile loci of the indica-japonica hybrid, namely S5, S7, S8 and S9. Indica-japonica fragments can be substituted by molecular markers of the fertility gene loci; the required fragments can be precisely and quickly introduced; the selection efficiency of fragment substitution of the indica-japonica fertility loci is greatly improved, and a japonica type indica-compatible light and temperature-sensitive male sterile line 509S can be bred.

Description

1. Technical field [0001] The invention provides a molecular marker method for indica-japonica fragment replacement of indica-japonica hybrid fertility sites, belonging to the field of molecular genetics. And use this method to breed japonica-type indica-tropic photothermo-sensitive male sterile line 509S. This method is suitable for the breeding of indica-japonica hybrid progeny fertility locus segment replacement lines, and can promote the utilization of heterosis among indica-japonica subspecies. 2. Background technology [0002] In recent years, with the continuous increase of my country's population and limited arable land, increasing rice yield is of great significance to ensure national food security, and the utilization of indica and japonica heterosis is an important way to increase rice yield. But the indica-japonica interspecies hybrid F 1 Usually exhibit partial sterility, which limits the effective use of its strong heterosis in production. The discovery of w...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01H1/02
Inventor 万建民赵志刚江玲陈亮明刘世家刘喜王益华
Owner NANJING AGRICULTURAL UNIVERSITY
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