30 results about "Tridacna" patented technology

The invention relates to a medication method of pearl nuclei. The technical proposal adopted by the method for solving the technical problems thereof is as follows: high-purity gelatin, tetracycline hydrochloride, polyvinylpyrrolidone (PVP) and vitamin C are weighed respectively as according to the mass ratio of 2-4%, 5-8%, 0.4-0.8% and 0.2-0.4%, added with sterilized seawater to 1,000ml, stirred in water bath at 50 DEG C and dissolved overnight; the pearl nuclei (clam or tridacna shells are ground to be round) are heated in a 50-60 DEG C thermotank to the temperature of 50-60 DEG C, the pearl nuclei are taken out, the overnight dissolved solution is put into a paint sprayer, and the surface of the pearl nuclei is sprayed and continuously turned over to cause the sprayed drug layer to be even, smooth and dry; the pearl nuclei are packaged in vacuum and transported to a pearl cultivation farm, pearl-culturing oyster are inserted into the pearl nuclei by the conventional nuclei insertion technology, and then post-operation recuperation and hanging cultivation are carried out. The medication method of the pearl nuclei has the advantages of effectively improving pearl nacre, lowering the mortality rate and nuclei spitting rate, improving the pearl output and economic benefit of breeding production, simple using method, easy operation and remarkable effect.

The invention discloses a micro-satellite primer used for identifying Tridacna maxima and Tridacna rosewateri, and an identification method. The sequence of the micro-satellite primer TC89 is F:5'-ACAAACGAAGCAGGTGTT-3', R:5'- GGTTCTGGATAAATGGGT-3'. The identification method is capable of realizing rapid and accurate identification of Tridacna maxima and Tridacna rosewateri, is simple, accurate, and effective, and is low in cost; the results are visual; no influence is caused by the environment or the growth period.

The invention discloses a tridacna larvae artificial breeding method capable of improving the survival rate during proliferation and releasing. The method includes the steps of: performing selection and treatment on tridacna larvae for artificial breeding, and transporting the treated tridacna larvae to a sea area for proliferation and releasing; taking the tridacna larvae out of a flowing water bin, performing semi-buried treatment on the taken tridacna larvae in a calcium carbonate-material releasing base, then putting the releasing base with the buried tridacna larvae in a mesh cage rack with a lid, fixing the mesh cage rack on the bottom of the sea, and keeping the distance between the mesh cage rack and the slack tide water level at 3-15 meters and the distance between the mesh cage rack and the bottom surface of a seabed base at 30 cm or above; when the length of shells of the tridacna larvae grows to 6 cm or above, removing the tridacna and mesh cage rack, putting the removed tridacna and mesh cage rack in a coral reef area which has smooth water flow, light intensity of 6,000-12,000 LX and flat parts for growth, cleaning covering matter in time in a typhoon season, performing recovery and fixation on rollover tridacna bases, and eliminating harmful organisms. Through the tridacna larvae artificial breeding method capable of improving the survival rate during proliferation and releasing, the survival rate of the tridacna larvae can be improved effectively, and technical support can be provided for proliferation of the tridacna resource and ecological restoration of coral reefs in China.

The invention discloses an identification primer suitable for hippopus and common tridacna varieties in South China Sea and hybrids thereof and a method thereof. The identification primer is a TC114 primer pair, and a forward primer and a reverse primer of the identification primer are as follows: F: 5'-AACACTGATTGGGCTCTT-3'R: 5'-TTGACGACGATGACGATA-3'. A pair of microsatellite primer TC114 primerpair is screened from 60 pairs of tridacna crocea microsatellite primers, can be universally used in hippopus and common tridacna varieties (tridacna derasa, tridacna squamosa, tridacna maxima, tridacna crocea and tridacna ovalvata) in South China, and can be distinguished according to the size of an amplified band, meanwhile, the microsatellite primer TC114 primer pair is clear in banding pattern, good in repeatability and high in reliability, and has potential application value in individual identification of hybrids between hippopus and common tridacna in South China Sea.

The invention discloses microsatellite primers, a kit and an identification method for identifying tridacna crocea, tridacna squamosa and hybrid first filial generations of the tridacna crocea and thetridacna squamosa. The microsatellite primers TC111 are represented as F: 5'-CCATTTTGGTGCGTAGTA-3' and R: 5'-CTTGAGGGCACCTTTTAG-3'. With the adoption of the method, the tridacna crocea, the tridacnasquamosa and the hybrid first filial generations of the tridacna crocea and the tridacna squamosa can be rapidly and accurately identified, the method has important actual application value, and meanwhile, the method has the advantages of being simple and low in cost, obtaining a direct, accurate and effective result, avoiding influence of the environment and the development stage and the like.

The invention discloses an artificial pearl nucleus as well as a preparation method and application thereof. A high polymer material polylactic acid with histocompatibility is used as a carrier, hydroxyapatite powder and nano silver powder are uniformly mixed according to a certain proportion, then an injection molding method is adopted to produce a pearl nucleus, and the pearl nucleus is boiled and purified for nucleus implantation and pearl cultivation production. The artificial pearl nucleus is simple in preparation process, controllable in shape, pure white in color and proper in density,and has good histocompatibility and antibacterial activity. By cultivating pearls through the artificial pearl nucleus, and the survival rate of pearl cultivating shells and the quality of the pearlscan be remarkably improved. The artificial pearl nucleus can replace the traditional tridacna or clam pearl nucleus to cultivate pearls.

The invention discloses a method for ascertaining degree of maturity of tridacna ovums. The method comprises the following steps: A, tridacna parent selection: selecting healthy and undamged individual tridacna as an ascertaining object of the degree of maturity of ovums and cleaning and temporarily rearing selected tridacna into seawater; B, collection of gonadal tissues: utilizing an injector tostretch from a tridacna water outlet hole, insert into a gonad surface-layer tissue of tridacna and absorb gonad tissue; C, ascertaining of degree of maturity: performing microscopic observation on the collected gonad tissue. Under a microscope, the observed condition of the degree of maturity of ovums comprises the following three types including an ovum-immaturity stage where ovums are shaped like teardrops or pearls and have the diameters not up to the specification of naturally-discharged ovums; an ovum-maturity stage where ovums are circular or elliptical and have the diameters identicalto ovums in sizes with yolk containing enriched accumulated matter and the ovums have double-layer smooth ovum membranes; and a ovum-maturity stage wherein ovums are circular and the ovum membranes wrinkle so that ovums are likely to crack, thereby making yolk overflow.

The invention discloses a tridacna household peculiar smell removing and air purifying device. The structure of the device comprises a tridacna cover, a control panel, a power interface and a collection cabinet, wherein the control panel is installed on the upper surface of the tridacna cover in a matched mode, the power interface is embedded in the right side of the tridacna cover, and the collection cabinet is connected to the front side of the tridacna cover in a movable clamping mode. The tridacna household peculiar smell removing and air purifying device has the beneficial effects that indoor air is sucked into the tridacna cover, then moisture, impurities and peculiar smell in the air are isolated, when a purification block in the tridacna cover needs to be replaced and washed, two sides of the tridacna cover are pressed to enable clamping plates to be bent inwards and squeeze supporting springs upwards, then the tridacna cover can be conveniently opened for replacing and washing the interior of the purification block, meanwhile, the moisture makes contact with the impurities to generate agglomerates which fall downwards, air flow can only push poking pieces to rotate in one direction under engagement of guide blocks and stop blocks, then the agglomerates are conveniently discharged into a storage groove through through grooves to be stored on the surface of a bottom plate, the agglomerates are prevented from being adhered to the interior of the tridacna cover, and the agglomerates can be cleaned by pulling out a storage box through the collection cabinet.

The invention discloses a chemical method for effectively avoiding self-fertilization of tridacna. The method includes: allocating ID numbers to all tridacna subjected to induction, respectively placing into a sperm collection drum, timing from spermination in large quantity, obtaining sperms after spermination for 2-3min, filtering through a 500-mesh bolting silk screen to obtain filtrate which is sperm fluid, and marking +ID numbers ; moving the tridacna from the sperm collection drum into an ovum collection drum containing medicated bath liquid, wherein the liquid level of the medicated bath liquid is at least higher than a highest position of the tridacna; after ovulation for 15-20min, taking out the tridacna to obtain ovum cell liquid, filtering the ovum cell liquid through a 300-mesh bolting silk screen to obtain ovum cells on the bolting silk screen, cleaning the ovum cells on the bolting silk screen with seawater, and marking +ID numbers; checking sperm and ovum gamete quality, abandoning low-quality gametes, and subjecting the sperms and the ova different in source according to the different ID numbers to artificial fertilization as required.

The invention belongs to the technical field of medicine and health care, and specifically relates to a traditional Chinese medicine extractive capable of inhibiting bacteria and strengthening teeth, and a preparation method and applications thereof. The traditional Chinese medicine extractive is composed of following ingredients, by weight, 5 to 60% of tridacna, 10 to 25% of rhizoma imperatae, 5 to 25% of honeysuckle stem, 5 to 10% of fructus forsythia, 5 to 10% of betel nut, and 15 to 25% of Lonicera japonica. According to the preparation method, the ingredients above are smashed and mixed; an obtained mixture is subjected to percolation extracting; and a percolation solvent is recycled so as to obtain the traditional Chinese medicine extractive. According to the traditional Chinese medicine extractive, which is a natural marine drug extractive, tridacna is taken as a main ingredient; the traditional Chinese medicine extractive is capable of strengthening teeth, inhibiting bacteria, and maintaining oral cavity health, is convenient for carrying, and is simple for using; the preparation method is simple; manufacturing is convenient; and it is beneficial for industrialized popularization and application.

The invention discloses identification primers, a kit and an identification method for tridacna crocea, tridacna maxima and a first filial generation of the tridacna crocea and the tridacna maxima. The identification primers are F:CCATTTTGGTGCGTAGTA and R:CTTGAGGGGCACCTTTTAG. According to the identification primers, the kit and the identification method, a pair of microsatellite primers TC111 which can be universally used in the tridacna crocea and the tridacna maxima and can be used for distinguishing two types of tridacna according to the size of an amplification band and have a clear band type, good repeatability and strong reliability is screened from transcriptome microsatellite primers of 150 pairs of the tridacna crocea; and the microsatellite primers TC111 have important application value in identification of individuals of the first filial generation of the tridacna crocea and the tridacna maxima.

The invention discloses a cement and coral sand mixed type substratum and a manufacturing and application method thereof. The light and environment-friendly cement and coral sand mixed type substratum is developed through the technical means of material preparation, substratum mold manufacturing, substratum manufacturing, substratum treatment, substratum application and the like. According to the method, the survival rate of the tridacna juvenile mollusks is increased, the growth speed of the juvenile mollusks is increased, and the breeding period is shortened. The method is different from the traditional corallite attachment base which is uneven and destroys coral reefs, and has the advantages of low cost, simplicity and convenience in operation, environment friendliness, easiness in popularization and the like.

The invention discloses an in-situ induced spawning method for giant clam parents. The method comprises the technical links of parent selection, in-situ spawning induction, larva examination, spawning induction effect evaluation and the like. The method is different from a tridacna full-artificial breeding method, a field semi-closed lagoon face and other environments are screened as an induced spawning field, in-situ induced spawning is conducted at night, the artificial fry production process is simulated as much as possible, risks and enemies are avoided, and artificial induced spawning fries consistent with wild fries are obtained in batches; and parent-child recognition is carried out by combining a modern molecular marker technology, and the in-situ induced spawning effect is evaluated. According to the method, the floating characteristic of the giant clam in the larva stage is fully utilized, large-area diffusion is achieved by means of sea waves, ocean currents and the like, a part of giant clam seed gathering area is formed, sufficient parents are provided for giant clam breeding in the future, and technical guarantee is provided for rapid restoration of giant clam resources in the South China Sea. The method has the advantages of simplicity, convenience, feasibility, remarkable effect and the like.

The invention discloses a method for artificially transplanting open sea island reef giant clams. The method comprises the following steps: fixing a metal culture cage at the seabed of a giant clam growth sea area, collecting corallite, filling the corallite in the metal culture cage, then placing giant clam in the corallite, enabling an outer sleeve film to face upwards, covering and locking a cover, and enabling the giant clam to grow. The device is high in water flow resistance, corrosion resistance, typhoon resistance and the like; even after one year, the survival rate of the tridacna can reach 60% or above without related maintenance. Therefore, the technical support can be provided for ecological restoration of the giant clam on islands far away from mainland, such as related islands in Nansha and Xiasha islands in China, and the technical guidance can also be provided for development of the giant clam culture industry.

The invention discloses a method for culturing tridacna juvenile mollusks through a hanging type net cage. The floating cutting culture method of the tridacna juvenile mollusks is created through the technical means of selection and attachment of the tridacna juvenile mollusks, manufacturing of the hanging type net cage, transportation of the tridacna juvenile mollusks, culture of the hanging type net cage, nursing management and the like. According to the method, the tridacna juvenile mollusks are bred in the selected sea area in a floating cutting and hanging breeding mode for the first time, the bottom sowing proliferation cost of the tridacna is greatly reduced, the proliferation efficiency and the growth speed of the tridacna are improved, joint restoration of various tridacna resources is achieved, and technical guarantee is provided for benign and healthy development, reef building and protection and the like of the South China Sea coral reef ecosystem in China. The method has the advantages of being high in practicability and easy to popularize.

The invention provides a tridacna mantle picture acquisition device and application method thereof, and belongs to the technical field of shellfish color measurement. The device includes a glass jar, a glass culture dish, a full spectrum halogen lamp, a camera fixing frame and a camera; black light-absorbing plastic films are utilized to perform sealing on the periphery and the outer part of the bottom surface of the glass jar; the glass culture dish is placed in the square glass jar, and holds white corallites whose particle size is 2 to 5mm and thickness is 2 to 3cm; the camera fixing frame is arranged outside the glass jar to enable a camera to be fixed right above the glass culture dish; and the full spectrum halogen lamp is fixed at one side in the glass jar, the height is 30cm, light irradiates the center of the culture dish downwards, light and the horizontal plane form an angle of 30 to 45 degrees, and the illumination intensity is 6000 to 8000lux. The invention also provides a method for using the abovementioned device to quantify the tridacna mantle color, and the method enables tridacna mantle color change research to be more scientized and normalized.