Method for ascertaining degree of maturity of tridacna ovums

Abstract

The invention discloses a method for ascertaining degree of maturity of tridacna ovums. The method comprises the following steps: A, tridacna parent selection: selecting healthy and undamged individual tridacna as an ascertaining object of the degree of maturity of ovums and cleaning and temporarily rearing selected tridacna into seawater; B, collection of gonadal tissues: utilizing an injector tostretch from a tridacna water outlet hole, insert into a gonad surface-layer tissue of tridacna and absorb gonad tissue; C, ascertaining of degree of maturity: performing microscopic observation on the collected gonad tissue. Under a microscope, the observed condition of the degree of maturity of ovums comprises the following three types including an ovum-immaturity stage where ovums are shaped like teardrops or pearls and have the diameters not up to the specification of naturally-discharged ovums; an ovum-maturity stage where ovums are circular or elliptical and have the diameters identicalto ovums in sizes with yolk containing enriched accumulated matter and the ovums have double-layer smooth ovum membranes; and a ovum-maturity stage wherein ovums are circular and the ovum membranes wrinkle so that ovums are likely to crack, thereby making yolk overflow.

Description

Technical field: [0001] The invention belongs to the field of shellfish genetic breeding in marine biotechnology, in particular to a method for identifying the maturity of giant clam eggs. Background technique: [0002] Tridacna belongs to the family Tridacnaidae, the mollusc Phylumbranchia, clams, and is mainly distributed in the coral reefs or shallow reefs near the low tide areas of the Indian Ocean, the Western Pacific Ocean and the South China Sea. In my country, the main species of giant clam include giant clam kushii, scaleless giant clam, scaled giant clam, long giant clam, nova giant clam, safflower giant clam and giant giant oyster of the genus Tridacna. With the popularization of diving equipment and motor boats, giant clam resources have been severely damaged. For example, giant clam kushii is almost extinct in the South my country Sea, and scaleless giant clam has become extremely rare. Other species of giant clam are still facing endangerment. The artificial br...

AI Technical Summary

Problems solved by technology

Among them, as long as the sperm is in a motile state, most of them are in the mature stage and have the ability to fertilize; while the maturity of the egg is the key factor determining the quality of the zygote, but since there is no significant phenotypic difference in the appearance of giant clams, it is difficult to directly judge the maturity of the egg Spend

In the past, judging the maturity of giant clam eggs was often done by dissection, which killed a large number of giant clam parents, resulting in serious parental loss.

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