Method for ascertaining degree of maturity of tridacna ovums

A technology of maturity and clam, which can be used in measuring devices, material analysis by optical means, instruments, etc., and can solve problems such as loss of parent shells, difficulty in egg maturity, etc.

Inactive Publication Date: 2018-09-11
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, as long as the sperm is in a motile state, most of them are in the mature stage and have the ability to fertilize; while the maturity of the egg is the key factor determining the quality of the zygote, but since there is no significant phenotypic differenc

Method used

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  • Method for ascertaining degree of maturity of tridacna ovums

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A. Tridacna parent selection: In May 2015, 10 giant clams were collected from Hainan and transported to the Hainan Tropical Marine Biology Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences. Sand-filtered seawater at a temperature of 26-28°C. Select a healthy and undamaged individual with a shell length of 30-40cm and an average weight of 5kg as the identification object of gonad maturity;

[0017] B. Collection of gonad tissue: put the selected giant clam parent into a glass fiber reinforced plastic bucket filled with seawater, and mark it well. Use a 5mL plastic syringe to extend into the outlet hole of the giant clam, insert it into the surface of the gonad, and draw 1.5mL of gonad tissue into a centrifuge tube to avoid puncturing the kidney or other important organs. After one instance was taken, the syringe and needle were rinsed with seawater and sterilized with alcohol before being used on another instance.

[0018]...

Embodiment 2

[0021] A. Tridacna parent selection: In June 2015, 10 giant clams were collected from Hainan and transported to the Hainan Tropical Marine Biology Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences. After cleaning, they were slightly inflated and temporarily raised at a salinity of 32ppt. Sand-filtered seawater at a temperature of 26-28°C. Select healthy and undamaged individuals with a shell length of 30-40cm and an average weight of 4kg as gonad maturity identification objects;

[0022] B. Collection of gonad tissue: put the selected giant clam parent into a fiberglass bucket filled with fresh seawater, and mark it. Use a 5mL plastic syringe to extend into the outlet hole of the giant clam, insert it into the surface of the gonad, and draw 1.5mL of gonad tissue into a centrifuge tube to avoid puncturing the kidney or other important organs. After one instance was taken, the syringe and needle were rinsed with seawater and steril...

Embodiment 3

[0026] A. Tridacna parent selection: In March 2016, 20 giant clam oysters were collected from Hainan and transported to the Hainan Tropical Marine Biology Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences. Sand filtered sea water at 26-28°C. Select a healthy and undamaged individual with a shell length of 30-40cm and an average weight of 5kg as the identification object of gonad maturity;

[0027] B. Collection of gonad tissue: put the selected giant clam parent into a fiberglass bucket filled with fresh seawater, and mark it. Use a 5mL plastic syringe to extend into the outlet hole of the giant clam, insert the superficial gonad tissue and draw 1.5ml of gonad tissue into a centrifuge tube to avoid puncturing the kidney or other important organs. After one instance was taken, the syringe and needle were rinsed with seawater and sterilized with alcohol before being used on another instance.

[0028] C. Microscope observation: Obs...

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Abstract

The invention discloses a method for ascertaining degree of maturity of tridacna ovums. The method comprises the following steps: A, tridacna parent selection: selecting healthy and undamged individual tridacna as an ascertaining object of the degree of maturity of ovums and cleaning and temporarily rearing selected tridacna into seawater; B, collection of gonadal tissues: utilizing an injector tostretch from a tridacna water outlet hole, insert into a gonad surface-layer tissue of tridacna and absorb gonad tissue; C, ascertaining of degree of maturity: performing microscopic observation on the collected gonad tissue. Under a microscope, the observed condition of the degree of maturity of ovums comprises the following three types including an ovum-immaturity stage where ovums are shaped like teardrops or pearls and have the diameters not up to the specification of naturally-discharged ovums; an ovum-maturity stage where ovums are circular or elliptical and have the diameters identicalto ovums in sizes with yolk containing enriched accumulated matter and the ovums have double-layer smooth ovum membranes; and a ovum-maturity stage wherein ovums are circular and the ovum membranes wrinkle so that ovums are likely to crack, thereby making yolk overflow.

Description

Technical field: [0001] The invention belongs to the field of shellfish genetic breeding in marine biotechnology, in particular to a method for identifying the maturity of giant clam eggs. Background technique: [0002] Tridacna belongs to the family Tridacnaidae, the mollusc Phylumbranchia, clams, and is mainly distributed in the coral reefs or shallow reefs near the low tide areas of the Indian Ocean, the Western Pacific Ocean and the South China Sea. In my country, the main species of giant clam include giant clam kushii, scaleless giant clam, scaled giant clam, long giant clam, nova giant clam, safflower giant clam and giant giant oyster of the genus Tridacna. With the popularization of diving equipment and motor boats, giant clam resources have been severely damaged. For example, giant clam kushii is almost extinct in the South my country Sea, and scaleless giant clam has become extremely rare. Other species of giant clam are still facing endangerment. The artificial br...

Claims

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Application Information

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IPC IPC(8): A01K61/54G01N21/84
CPCA01K61/54G01N21/84Y02A40/81
Inventor 李军张跃环张扬肖述向志明马海涛喻子牛
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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