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31 results about "Gonad tissue" patented technology
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Method for producing early gonad paraffin section of Chinese soft shell turtle
ActiveCN103954484AWide variety of sourcesLow costPreparing sample for investigationEmbryoPre embedding
The invention relates to a method for producing an early gonad paraffin section of Chinese soft shell turtle, which comprises the following steps: 1)fixing a sample, 2)dehydrating and transparentizing, 3)performing paraffin infiltration and embedding, 4)slicing and spreading the section, and 5)dyeing and sealing the section. The method is characterized in that the sample in the step 1) is embedded in a bean product pretreated by a stationary liquid. Compared with prior art, the method has the advantages that the bean product can be taken as a pre-embedding material for embryo stage gonad paraffin section of Chinese soft shell turtle, the source is wide, cost is low, through the pre-embedding, the embryo stage gonad can be better embedded in bean product, and subsequent tissue embedding and slicing are not influenced. The method avoids the disadvantages in a traditional method, and overcomes the disadvantages of difficult operation and observation of early gonad paraffin section of Chinese soft shell turtle; in addition, the pre-embedding gonad position is fixed and several gonad tissue can be processed at a time, so that large scale slicing and analysis are convenient to operate, and the method is especially suitable for researches by aiming at different individuals.
Owner:ZHEJIANG WANLI UNIV
Construction and application method of cynoglossus semilaevis pseudo male gonad cell line
InactiveCN104152402AMicroorganism based processesArtificial cell constructsCynoglossus semilaevisEstablished cell line
The invention aims to provide a construction method of a cynoglossus semilaevis pseudo male gonad cell line, thereby establishing the cynoglossus semilaevis pseudo male gonad cell line and providing a basis of researching sex differentiation and sex reversal regulatory mechanism of cynoglossus semilaevis. In the construction method, the cynoglossus semilaevis pseudo male gonad cell line is successfully established by employing cynoglossus semilaevis pseudo male gonad tissue. Continuous passage of the established cell line can be achieved. By means of the method, the passage cells can be generated for more than 60 generations and a large amount of cynoglossus semilaevis pseudo male gonad cells can be provided. The method can be widely applied in researches of exogenous gene, transfection of plasmids and functions of sex relative genes, can be applied in researches of breeding of common viruses in fish and a cooperative action mechanism of cells, and can also be applied in detection of environmental pollutants. The method has an important practical application value.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Juvenile pomfret gonad fixing and obtaining method
InactiveCN102217564AEasy to getSimple processClimate change adaptationPisciculture and aquariaJuvenile fishCuticle
The invention relates to a juvenile pomfret gonad fixing and obtaining method including the steps as follows: firstly, determining fresh dead juvenile pomfret, cutting the belly epidermis of the fresh dead juvenile pomfret to open, cutting away partial tissues and organs which do not contain gonad, then fixing the part containing gonad with Bouin fixative, at last, taking out the fixed tissue block and finding the gonad tissue of the pomfret behind the stomach intestine visceral mass under an anatomical lens. The juvenile pomfret gonad fixing and obtaining method can be used for sectioning inthe gonad histology of the juvenile pomfret and also applicable to research on the gonad histology of other aquatic fishes; and the juvenile pomfret gonad fixing and obtaining method has broad applicability.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Sex identification method for juvenile fish of hybrid pelteobagrus fulvidraco (richardson)
InactiveCN103529036AEfficient identificationRapid identificationPreparing sample for investigationMaterial analysis by optical meansJuvenile fishStaining
The invention discloses a sex identification method for juvenile fish of hybrid pelteobagrus fulvidraco (richardson) [pelteobagrus fulvidraco (richardson) (male)* pelteobagrus vachelli (richardson) (female)]. The hybrid pelteobagrus fulvidraco (richardson) having an individual body length of above 3 cm is identified by utilization of a method of living-body gonad squashing, acetocarmine staining and ethanol crystal violet staining, and the accuracy of the method is verified by a paraffin section of the gonad tissue. The sex identification method is suitable for sex identification for juvenile fish of common pelteobagrus fulvidraco (richardson) and of "all-male No.1" pelteobagrus fulvidraco (richardson). By the sex identification method, sex identification of one juvenile fish can be completed in 3 min, and 100 fishes can be identified by cooperation of two persons for 2 h. The sex identification method has characteristics of high efficiency, economy, rapidness, accuracy and stability. The sex identification method has important application value for male fry cultivation of the pelteobagrus fulvidraco (richardson) and the hybrid pelteobagrus fulvidraco (richardson) thereof, and can be applied for rapid sex identification of a pelteobagrus fulvidraco (richardson) commodity summer fry.
Owner:NANJING NORMAL UNIVERSITY
Andrias davidianus female specific marker and applications thereof
ActiveCN108192979ASolving the problem of giant salamander sex identificationSimple technologyMicrobiological testing/measurementDNA/RNA fragmentationChinese giant salamanderNucleotide
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Separation method and culture medium for ricefield eel germline stem cells
ActiveCN112251399AHigh purityTypical Stem Cell MorphologyCell dissociation methodsClimate change adaptationAlkaline phosphatase stainingBiochemistry
The invention relates to a culture medium for ricefield eel germline stem cells. The culture medium comprises a basic culture medium and FBS, wherein the concentration of the FBS is 5-8%. The invention also relates to a method for separating and culturing the ricefield eel germline stem cells. The method comprises the step of culturing the ricefield eel germline stem cells by using the culture medium. According to the culture medium disclosed by the invention, the adherence efficiency of the ricefield eel germline stem cells can be improved, differentiation of the ricefield eel germline stem cells is prevented, and the proliferation function of the ricefield eel germline stem cells is not influenced. By the method disclosed by the invention, ricefield eel female germline stem cells and male germline stem cells which have higher purity can be separated and obtained in five days by using gonad tissues of ricefield eels. The obtained germline stem cells have typical stem cell morphology,typical stem cell clone can be formed, alkaline phosphatase staining is positive, and related molecular markers of the stem cells are also highly expressed. After the germline stem cells obtained by the method disclosed by the invention are cryopreserved, the germline stem cells can be successfully recovered.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Method for evaluating PBDEs xenoestrogen effect by using gobiocyprisrarus gene expression
InactiveCN101875969AObvious estrogen-like effectMicrobiological testing/measurementXenoestrogenBiology
The invention discloses a method for evaluating a PBDEs xenoestrogen effect by using gobiocyprisrarus gene expression, and relates to the evaluation of the influence of water environmental pollution on hydrobios. In the method, the expression quantity of CYP19b genes in the brain tissue and gonad tissue of gobiocyprisrarus cultivated in water with different amounts of PBDEs is detected to illustrate that the PBDEs content of the water is higher, the xenoestrogen effect is more obvious, so that the method for evaluating the PBDEs xenoestrogen effect is obtained.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Method for ascertaining degree of maturity of tridacna ovums
InactiveCN108513934AFast non-destructive identificationClimate change adaptationMaterial analysis by optical meansWrinkle skinYolk
The invention discloses a method for ascertaining degree of maturity of tridacna ovums. The method comprises the following steps: A, tridacna parent selection: selecting healthy and undamged individual tridacna as an ascertaining object of the degree of maturity of ovums and cleaning and temporarily rearing selected tridacna into seawater; B, collection of gonadal tissues: utilizing an injector tostretch from a tridacna water outlet hole, insert into a gonad surface-layer tissue of tridacna and absorb gonad tissue; C, ascertaining of degree of maturity: performing microscopic observation on the collected gonad tissue. Under a microscope, the observed condition of the degree of maturity of ovums comprises the following three types including an ovum-immaturity stage where ovums are shaped like teardrops or pearls and have the diameters not up to the specification of naturally-discharged ovums; an ovum-maturity stage where ovums are circular or elliptical and have the diameters identicalto ovums in sizes with yolk containing enriched accumulated matter and the ovums have double-layer smooth ovum membranes; and a ovum-maturity stage wherein ovums are circular and the ovum membranes wrinkle so that ovums are likely to crack, thereby making yolk overflow.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Establishment and application of coilia ectenes gonad somatic cell line
The invention relates to the technical field of biology, in particular to establishment and application of a coilia ectenes gonad somatic cell line. The method comprises the following steps: (1) separating and digesting gonad tissues of coilia ectenes, and collecting the cells for plank culturing; and (2) after the cells are adhered to the wall, carrying out passage culture for 45-60 generations in sequence. The obtained coilia ectenes gonad somatic cell line can be stably passaged and proliferated, and can induce fish spermatogonial stem cells to differentiate in vitro to generate sperms.
Owner:SHANGHAI OCEAN UNIV
Early sex identification method for tortoises
The invention relates to an early sex identification method for tortoises, belonging to the field of sex differentiation and sex adjustment and control of temperature-dependent sex determination animals. After being weighed, young tortoises, which are two weeks old, are immersed by ethyl alcohol; then, external morphological parameters are acquired and measured; male and female young tortoise gonads are observed; found from tissue section observation, the sexes are determined; external characteristic parameters of the tortoises obviously related to the sexes are obtained through statistical analysis; therefore, a regression equation is obtained; and the sexes of the newborn tortoises can be judged through the regression equation. Animals do not need to be slaughtered; sex identification of the young tortoises, which are about two weeks old, can be carried out through appearance; compared with a gonad tissue section method, the method is simple, high-efficiency and simple to operate; the accuracy rate is higher than 95%; the operability is high; tools required for performing sex identification are simple and easy to obtain; operation is simple; and the tools include a vernier caliper and a computer.
Owner:SHENZHEN POLYTECHNIC
Method for identifying physiological sex of fish at early development stage by using 5SrRNA
The invention discloses a method for identifying the physiological sex of fish at an early development stage by using 5S rRNA, which comprises the following steps: (1) extracting the total RNA of fish gonad tissues by using a method for conventionally extracting the total RNA of animal cells by using a total RNA extraction kit or a TRIzol reagent, determining an OD260 / 280 value by using a nucleic acid analyzer, and judging the RNA purity, and then separating total RNA by using an agarose gel electrophoresis method, and observing the brightness and distribution of 5S rRNA, 18S rRNA and 28S rRNA; (2) carrying out microcapillary electrophoresis on the extracted gonad total RNA, and calculating the proportion of 5S rRNA in the total RNA according to the RNA type coverage area obtained by nucleic acid analysis software; (3) according to the proportion of the 5S rRNA in the total RNA, judging the physiological male and female of the fish at the early development stage, when the proportion of the 5S rRNA is smaller than or equal to 9.0%, judging the fish as a testis tissue, and when the proportion of the 5S rRNA is larger than or equal to 12%, judging the fish as an ovarian tissue. The method is easy to implement and simple and convenient to operate, the physiological sex identification of more than 100 fish individuals is realized within 12 hours, and the physiological sex of the fish is determined.
Owner:HUAZHONG AGRI UNIV
A kind of preparation method of frozen section of penaeus vannamei gonad tissue
ActiveCN110274807BImprove slicing efficiencyShorten the timeWithdrawing sample devicesPreparing sample for investigationStainingMelicertus
Owner:ZHEJIANG MARICULTURE RES INST
Preparation method of frozen sections of gonad tissues of penaeus vannamei boone
ActiveCN110274807AImprove slicing efficiencyShorten the timeWithdrawing sample devicesPreparing sample for investigationFresh TissueGonad tissue
The invention discloses a preparation method of frozen sections of gonad tissues of penaeus vannamei boone. The preparation method comprises the following steps: tissue dehydrating after formalin fixation, tissue embedding, section freezing, section dyeing and sealing. The method provided by the invention is different from a freezing slicing technology of fresh tissues aiming at a freezing slicing technology of penaeus vannamei boone gonad tissues fixed by formaldehyde. Compared with a traditional paraffin slicing method, the method has higher slicing efficiency, and saves the time. Compared with a common freezing slicing method, the method has a better slicing effect and is beneficial to staining observation.
Owner:ZHEJIANG MARICULTURE RES INST
A method for separating and purifying flounder oocytes
ActiveCN108265026BLittle loss in quantityEasy to operateCell dissociation methodsGerm cellsOogoniumConcentration gradient
The invention relates to a method for separating and purifying oogonia of paralichthys olivaceus. The method comprises the following steps of 1), stripping tunica albuginea from the gonadal tissue offemale paralichthys olivaceus, cutting up, centrifuging, then digesting an obtained deposit by using combined enzyme liquid, filtering obtained cell digestive juice through a cell screen, centrifuging, and then resuspending to obtain cell suspension, wherein the combined enzyme liquid is prepared from trypsin and a DNA (Deoxyribonucleic Acid) enzyme I; 2), preparing Percoll liquid under physiological osmotic pressure to different concentration gradients, lightly flowing each level of well prepared Percoll gradient liquid into a centrifugal tube along a tube wall layer by layer from a high gradient to a low gradient to prepare for forming Percoll gradient liquid, and putting the cell suspension on the uppermost layer of the gradient; centrifuging, and then absorbing two uppermost layers ofcell bands to obtain the oogonia of the paralichthys olivaceus. The method provided by the invention is easy to operate; the whole operation is controllable; the oogonia of the paralichthys olivaceuscan be successfully separated out by adopting the method; the proportion of the oogonia is 80 percents or above, and the quantitative loss of the oogonia before and after purification is low.
Owner:中国水产科学研究院北戴河中心实验站
A kind of cold water purification cultivation method of female fish as raw material for processing sturgeon caviar
ActiveCN109452202BEasy to separateIncrease elasticitySpecific water treatment objectivesWater contaminantsBiotechnologyAnimal science
The invention relates to a cold-water purification female fish cultivation method for raw processing materials of sturgeon caviar. Through manual construction of a land cold-water quiet rearing base,equipping a low-temperature cold water system and extraction of reservoir bottom-layer water, a required cold water resource is provided and controlled. Through the combination of a breeding method ofbittern and feed deprivation, development of the gonad to the processible standard can be promoted, and roes are more easily separated from gonadal tissue when processed; meanwhile, the elasticity ofthe roes is improved, and the flavor and mouthfeel are a caviar product are promoted.
Owner:HANGZHOU QIANDAOHU XUNLONG SCI TECH +1
New use of Chinese medicine compound pharmaceutical composition
ActiveCN101214338BHigh activityNo effect on proliferationUnknown materialsSexual disorderAromataseRhizome
The present invention provides a medicine compound including the following raw materials with the weight portions such as 18 to 30 portions of prepared rehmannia rhizome, 9 to 12 portions of cornus dogwood fruit, 8 to 16 portions of Chinese yam, 9 to 12 portions of medlar, 8 to 16 portions of field dodder and 8 to 16 portions of deerhorn glue, and the purpose in preparing the medicine for delaying vaginal aging. The medicine is an active medicine for strengthening external gonad tissue aromatase and a medicine for increasing vaginal blood supply. The medicine is a medicine for preventing or / and curing senile vaginitis. Under a specific diagnosis standard, the medicine provided by the present invention has definite efficacy. The medicine has the advantages of same efficacy on delaying vaginal aging, few raw materials, wide sources, effective cost saving due to selection of raw materials, no effect on the level of serum estrogen after long-term administration, no proliferation on endometrium and safety. The present invention provides a new choice for clinically curing senile vaginitis, in particular preventing senile vaginitis or recurrence of senile vaginitis.
Owner:CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
Manufacturing method of macrobrachium rosenbergii gonad tissue frozen section
PendingCN114414346AImprove slicing efficiencyComplete organizational formPreparing sample for investigationPolyoxymethyleneStaining
The invention discloses a manufacturing method of a macrobrachium rosenbergii gonad tissue frozen section, a method for manufacturing the macrobrachium rosenbergii gonad tissue frozen section through a frozen section technology and application of the macrobrachium rosenbergii gonad tissue frozen section. The macrobrachium rosenbergii gonad tissue freezing and slicing method mainly comprises the steps of pretreatment of a glass slide before slicing, fixation of tissues by 4% paraformaldehyde, tissue embedding pretreatment, embedding, freezing and slicing and HE dyeing. Compared with a paraffin sectioning technology, the method has higher sectioning efficiency, and compared with a conventional freezing sectioning technology, the method has a more complete tissue form.
Owner:徐海晶
A method for developing an ecologically safe transgenic fluorescent ornamental fish
ActiveCN105063088BVector-based foreign material introductionAnimal husbandryEcological safetyWhole body
The invention discloses a fluorescent protein expression vector, methods for constructing and applying the fluorescent protein expression vector, a sexual gonad tissue specific regulation and expression vector, methods for constructing and applying the sexual gonad tissue specific regulation and expression vector and a method for cultivating ecological safety type transgenic fluorescent ornamental fish. Fluorescent proteins can be driven by the aid of promoters CMV with whole-body general expression functions to be expressed in the ornamental fish, and whole-body expression functions of the ornamental fish can be realized by the aid of the fluorescent protein expression vector. Reverse tetracycline-controlled transactivators rtTA can be driven by sexual gonad specific promoters kop to be expressed, and TRE promoters can be driven to start downstream Cre protein expression plasmid vectors after the reverse tetracycline-controlled transactivators rtTA and tetracycline are combined with one another. Sexual gonad of the ecological safety type transgenic fluorescent ornamental fish cultivated by the aid of the method does not contain transgenic components after the ecological safety type transgenic fluorescent ornamental fish is authenticated.
Owner:CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
Hyriopsis cumingii gender identification method
PendingCN112481388AEasy to judgeLower requirementMicrobiological testing/measurementPhysiologyMale gonad
The invention provides a hyriopsis cumingii gender identification method, and the method comprises: collecting a small amount of gonad tissue samples, and extracting DNA; designing a primer accordingto an excessive specific sequence of the M-type COII gene for PCR amplification; finally, observing strip results through agarose gel electrophoresis to judge male and female hyriopsis cumingii, and judging the hyriopsis cumingii with strips as male hyriopsis cumingii and the hyriopsis cumingii without strips as female hyriopsis cumingii. According to the method, the gender of hyriopsis cumingii is accurately identified mainly according to the genome amphiphilic and monophilic heredity phenomenon of hyriopsis cumingii and the characteristic that COII genes in an M-type mitochondrial genome arespecifically expressed in male gonads, and the result is simple and intuitive, so that the gender of hyriopsis cumingii is accurately identified; therefore, the method can be used for effectively distinguishing genders of early-stage fry of hyriopsis cumingii, and is less limited by samples and high in accuracy; besides, the DNA sample is used for judging more quickly and simply through gel electrophoresis, a subsequent sequencing result is not needed, and the detection time is greatly shortened, so that the detection efficiency is improved.
Owner:SHANGHAI OCEAN UNIV
A method for identifying the sex and gonad maturity of Apostichopus japonicus
InactiveCN106259081BReduce lossReduce volumeClimate change adaptationPisciculture and aquariaPlant Germ CellsBiology
The invention discloses an identification method for the sex and the gonad maturity degree of apostichopus japonicus, and belongs to the technical field of aquaculture. Parts of gonad tissue of anesthetized apostichopus japonicus are obtained through a negative-pressure tissue collector, the sex and the gonad maturity degree of apostichopus japonicus are identified according to the colors of female and male glands of apostichopus japonicus and the species, the size, the quantity and the structural characteristics of germ cells of female and male glands of apostichopus japonicus. The sex and the gonad maturity degree of apostichopus japonicus are identified with the method, a trauma is small, apostichopus japonicus parents do not need to be dissected, and losses of parents and the economic losses are reduced.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
A Method for Isolating and Cultivating Germ Cells from Gonad Tissue of Mud clam
ActiveCN110777109BStable survivalImprove disinfection and sterilization effectCell dissociation methodsInvertebrate cellsPlant Germ CellsMale gonad
The invention discloses a method for isolating and cultivating germ cells of gonad tissue of cockles. First, the gonad tissue of cockles is sucked with a sterile syringe under aseptic conditions, sterilized, and a mixed solution containing 0.05% trypsin and 0.2% collagenase is used at 23°C. Digest and enzymolyze gonadal tissue under certain conditions, collect the cell suspension, centrifuge, remove the supernatant, and suspend the precipitated cells with sterile buffer. The horizontal centrifugation speed was 1600rpm / m, and the cell suspension was taken, spread on a 6-well plate, and cultured upside down in a biochemical incubator at 26°C for 24 hours, then placed upright, and 1 mL of medium was added to each well, and half of the medium was replaced every 2 days thereafter. Constant temperature static culture for 5-10 days for primary culture, the present invention selects the culture conditions of gonad tissue of clam through a large number of basic experiments, so that the gonad cells can be isolated, purified, cultured and survived in vitro.
Owner:ZHEJIANG MARICULTURE RES INST
DsRNA of oncorhynchus mykiss ccne1 gene and application thereof
PendingCN114875030AReduce expressionEfficient combinationClimate change adaptationMicroinjection basedPathway analysisJuvenile fish
The invention discloses dsRNA of oncorhynchus mykiss ccne1 gene and application of the dsRNA, and belongs to the technical field of biology. The invention provides a rainbow trout ccne1 gene, an interference gene segment and dsRNA of the rainbow trout ccne1 gene. The method comprises the following steps: firstly, cloning by taking total RNA (Ribonucleic Acid) of gonad tissues of juvenile rainbow trout as a template to obtain a gene sequence of ccne1, wherein the nucleotide sequence of the gene sequence is SEQ ID NO.1, and the amino acid sequence of the gene sequence is SEQ ID NO.2; a ccne1 gene sequence is used as a template to design a primer of an interference gene segment, the interference gene segment is obtained, the nucleotide sequence is SEQ ID NO.3, and the dsRNA of the rainbow trout ccne1 gene is synthesized through in-vitro transcription. The dsRNA of the oncorhynchus mykiss ccne1 gene can efficiently silence the ccne1 gene and effectively reduce the expression of other meiosis related genes in oncorhynchus mykiss, and provides a research idea for oncorhynchus mykiss meiosis pathway analysis.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Identification method for sex and gonad maturity degree of apostichopus japonicus
InactiveCN106259081AReduce lossReduce volumeClimate change adaptationPisciculture and aquariaPlant Germ CellsBiology
The invention discloses an identification method for the sex and the gonad maturity degree of apostichopus japonicus, and belongs to the technical field of aquaculture. Parts of gonad tissue of anesthetized apostichopus japonicus are obtained through a negative-pressure tissue collector, the sex and the gonad maturity degree of apostichopus japonicus are identified according to the colors of female and male glands of apostichopus japonicus and the species, the size, the quantity and the structural characteristics of germ cells of female and male glands of apostichopus japonicus. The sex and the gonad maturity degree of apostichopus japonicus are identified with the method, a trauma is small, apostichopus japonicus parents do not need to be dissected, and losses of parents and the economic losses are reduced.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
A cryopreservation solution of sturgeon gonad tissue and a method for cryopreservation and recovery
ActiveCN111567515BLong-term effective preservationLong-term effective reproductive stem cell propertiesDead animal preservationArtificial cell constructsBiotechnologyAmerican paddlefish
The invention provides a cryopreservation solution of sturgeon gonad tissue and a method for cryopreservation and recovery. The present invention first takes the cultured fish as the object, systematically researches and screens out a cryopreservation solution with relatively simple components and a simple cryopreservation method, and proves the reproductive stem cell characteristics of gonad cells prepared after resuscitation through germ cell transplantation experiments , and also applied this method to the cryopreservation of the testis of the endangered fish Acipenser sinensis. Utilize the method provided by the present invention to carry out ultra-low temperature cryopreservation of sturgeon gonad tissue, prepare gonad single cell suspension after recovery, the cell activity can reach more than 90%, and there is no difference in the activity of cells prepared from fresh gonads; further effective through germ cell transplantation technology The germline stem cell properties of gonad cells were assessed. The cryopreservation solution of the invention has simple components and convenient operation of the cryopreservation method, and provides a new and effective way for long-term preservation and species recovery of endangered sturgeon germplasm resources.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Preparation method of sea urchin peptide oral liquid
InactiveCN106636067AOvercome limitationsOvercome the problem of limited production raw materialsVector-based foreign material introductionDNA preparationUltrafiltrationSea urchin
The invention discloses a preparation method of sea urchin peptide oral liquid and aims at solving the problems that sea-urchin gonad can be easily influenced by catching season and the like when being taken as a raw material in the prior art and production cost is high. The preparation method comprises the following steps: selecting sea-urchin gonad tissues, and obtaining RNA of sea-urchin gonad cells by adopting a chloroform extraction method; then adopting an RT-PCR method to obtain cDNA of the sea-urchin gonad cells, then carrying out PCR by utilizing a random primer to obtain all kinds of random gene fragments, then cloning the obtained gene fragments into a prokaryotic expression vector, carrying out induced expression to obtain random protein encoded by a random gene, carrying out protease digestion, and carrying out ultrafiltration to obtain sea urchin peptide solution; and blending the sea urchin peptide solution with wolfberry extract and mint extract to obtain the sea urchin peptide oral liquid. The obtained oral liquid is rich in lycium barbarum polysaccharide, organic acid, glycosides and flavones, taste is mellow, sweetness and acidity are moderate, antioxidant activity is good, and nutritional value is high; and technology is reasonable, operation is easy, production cost is low, and large-scale production can be easily realized.
Owner:山东明鑫集团有限公司
Juvenile pomfret gonad fixing and obtaining method
InactiveCN102217564BEasy to getSimple processClimate change adaptationPisciculture and aquariaJuvenile fishCuticle
The invention relates to a juvenile pomfret gonad fixing and obtaining method including the steps as follows: firstly, determining fresh dead juvenile pomfret, cutting the belly epidermis of the fresh dead juvenile pomfret to open, cutting away partial tissues and organs which do not contain gonad, then fixing the part containing gonad with Bouin fixative, at last, taking out the fixed tissue block and finding the gonad tissue of the pomfret behind the stomach intestine visceral mass under an anatomical lens. The juvenile pomfret gonad fixing and obtaining method can be used for sectioning inthe gonad histology of the juvenile pomfret and also applicable to research on the gonad histology of other aquatic fishes; and the juvenile pomfret gonad fixing and obtaining method has broad applicability.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Children chest X-ray fixation aid device for imaging departments
PendingCN110141264AGuaranteed therapeutic effectMove to preventPatient positioning for diagnosticsRadiation safety meansX-rayChest region
The present invention discloses a children chest X-ray fixation aid device for imaging departments. The children chest X-ray fixation aid device comprises a bracket, a first radiation shielding plate,connecting rods, a second radiation shielding plate, a sliding mechanism and a fixing mechanism. The children chest X-ray fixation aid device for the imaging departments can conduct a position restraining and fixing treatment on both arms of child patients, in addition, during a chest X-ray process of the child patients, a monitoring end of a chest X-ray apparatus only conducts ray irradiation ofchests of the child patients, heads and gonad tissues of the child parents are not subjected to the ray irradiation by the monitoring end of the chest X-ray apparatus due to shielding of the first radiation shielding plate and the second radiation shielding plate, and thus the device can protect the heads and the gland tissues of the child patients, can also prevent the arms of the child patientsfrom moving to the chest positions and ensures chest X-ray treatment effects of the child patients.
Owner:THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
Separation and culture method for germ cells of gonadal tissue of tegillarca granosa
ActiveCN110777109AStable survivalImprove disinfection and sterilization effectCell dissociation methodsInvertebrate cellsBiotechnologyPlant Germ Cells
The invention discloses a separation and culture method for germ cells of gonadal tissue of tegillarca granosa. The separation and culture method comprises the following steps: firstly, sucking gonadal tissue of tegillarca granosa by a sterile injector under a sterile condition, carrying out disinfection, performing digestive enzymolysis on the gonadal tissue at 23 DEG C with a mixed solution containing 0.05% of pancreatin and 0.2% of collagenase, collecting a cell suspension, carrying out centrifugation, removing a supernatant, suspending precipitated cells with a disinfection buffer solution, controlling the horizontal centrifugation speed at 2600 rpm / m when taking sperm cells from male gonadal tissue, controlling the horizontal centrifugation speed at 1600 rpm / m when taking egg cells from female gonadal tissue, taking the cell suspension, paving a 6-hole plate, carrying inverted adherent culture in a biochemical culture box at 26 DEG C for 24 h, then setting the plate right, adding1 mL of culture medium to each hole, then, replacing a half of culture medium every 2 d, carrying out constant-temperature standing culture for 5-10 d, and performing primary culture. The separation and culture method selects culture conditions of the gonadal tissue of the tegillarca granosa through a large quantity of basic experiments, so that gonadal cells can be separated, purified, cultured and survive in vitro.
Owner:ZHEJIANG MARICULTURE RES INST
A female-specific marker of Chinese giant salamander and its application
ActiveCN108192979BMicrobiological testing/measurementDNA/RNA fragmentationChinese giant salamanderNucleotide
The invention discloses an Andrias davidianus female specific marker and applications thereof. The steps comprise: A, cloning and verifying a specific fragment: 1) analyzing Andrias davidianus RAD candidate female specific fragments, 2) recovering a PCR product, cloning, and sequencing, and 3) analyzing the cloned and sequenced sequence; B, identifying physiological gender through tissue slicing:1) fixing and storing a collected Andrias davidianus gonad sample, 2) carrying out step-by-step ethanol dehydration on the sample, and 3) slicing the gonad tissue, and observing and identifying the physiological gender of Andrias davidianus; C, establishing a genetic sex identification system: 1) extracting genomic DNA, 2) design specific primers, wherein a nucleotide sequence represented by the verified sequence SEQ ID NO.1 is cloned, and 3) carrying out PCR amplification according to the reaction system; and D, applying the specific molecular marker in the identification of common individuals and sex reversal individuals of Andrias davidianus. According to the present invention, the marker has advantages of simple operation, accuracy and reliability, and can identify the genetic gender of Andrias davidianus through the non-invasive sampling; and the system for identifying the gender of Andrias davidianus and the sex reversal Andrias davidianus is firstly established, the simple and easy-performing molecular biology method is provided for the genetic gender identification of Andrias davidianus, and the approach is provided for the subsequent gender control breeding of Andrias davidianus.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Temperature regulating method for silvery pomfret parents strengthening culturing
InactiveCN107125169APromote nutrient accumulationImprove reproductive performanceClimate change adaptationPisciculture and aquariaWater temperatureGonad tissue
The invention relates to a temperature regulating method for silvery pomfret parents strengthening culturing. The method comprises the following steps: naturally reducing the water temperature of a silvery pomfret parents culturing pond to an interval of 12 to 13 DEG C, and keeping the water temperature at 12 to 13 DEG C for one week through heating; increasing the water temperature of the culturing pond to 18 to 19 DEG C according to an increasing amplitude of 0.4 to 0.6 DEG C every day; keeping the water temperature in the culture pond at 18 to 19 DEG C till the middle ten days and the last ten days of April in the next year, so as to ensure spawning and fertilization of silvery pomfret parents. The method can effectively promote the accumulation of nutrient in gonad tissues of the pomfret parents, obvioussy increases gonad index, fertilized egg quality and hatching rate, and finally improves the reproductive performance of silvery pomfret.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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