The invention discloses
antibacterial protein secreted by a
lactic acid tablet and a separation and identification method of the
antibacterial protein. The preparation method of the
lactic acid tablet specifically comprises the following steps: preparing
fermentation supernate of
pediococcus acidilactici YH-15; comprising the following steps: preparing a whey culture medium, inoculating and culturing YH-15, and preparing and storing
fermentation supernatant. Carrying out separation and purification and characterization detection on the
bacteriocin-like substance; comprising the steps of
salting-out of saturated
ammonium sulfate protein, purification of
protein through hydrophobic
column chromatography,
dialysis and freeze-
drying, and relates to the technical field of
protein spectrum identification, according to the
antibacterial protein secreted by the
lactic acid tablet and the separation and identification method thereof, a protein sample is subjected to enzymolysis through an FASP enzymolysis method, and after reduction and
alkylation centrifugal treatment is conducted by adding a
reagent, the protein sample is subjected to freeze-
drying;
ammonium bicarbonate and pancreatin are used for enzymolysis,
peptide fragments after enzymolysis are collected in a centrifugal mode, high-quality
peptide fragment raw materials are provided for identification of the protein spectrum,
impurity dry burning is reduced, and meanwhile the identification precision of the protein spectrum of a protein sample is effectively improved.