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Microsatellite primers, kits and identification methods for identifying Tridacna safranatum, Tridacna scales and their hybrid offspring

A technology of safflower clam and identification method, which is applied in biochemical equipment and methods, microbe measurement/inspection, DNA/RNA fragments, etc., and can solve the problems of difficult identification of hybrid species, morphological characteristics of safflower clam and squamous clam Difficult to distinguish and other problems, to achieve intuitive results, simple methods, and good repeatability

Active Publication Date: 2021-07-27
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

Hybrid species are even more difficult to identify because of the indistinguishability between safflower and scale clams in the juvenile stage based on morphological characteristics

Method used

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  • Microsatellite primers, kits and identification methods for identifying Tridacna safranatum, Tridacna scales and their hybrid offspring

Examples

Experimental program
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Embodiment 1

[0021] A small amount of mantle tissue was cut from the pore of giant clam safflower, giant clam scale and their hybrids, and 80 individual samples of each of giant clam safflower, giant clam scale and their hybrid offspring were extracted by phenol-chloroform extraction. Genomic DNA. Fully cut up the adductor muscle, absorb the water with clean filter paper, put it into a 1.5mL centrifuge tube, then add 400μL lysate and 10μL proteinase K (10mg / mL), mix well on a shaker, and digest in a water bath at 55°C 3 to 5 hours until the lysate is clear. Add an equal volume of 200 μL of saturated phenol and 200 μL of chloroform / isoamyl alcohol mixture (24:1 v / v) and extract three times. Precipitate DNA with 1 mL of absolute ethanol, wash with 70% alcohol, dry at room temperature and dissolve in 100 μL of ultrapure water. The concentration and purity of DNA were detected by ultraviolet spectrophotometer, and the integrity of DNA was detected by 1% agarose gel electrophoresis. Using th...

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Abstract

The invention discloses a microsatellite primer, a kit and an identification method for identifying giant clam safflower, giant clam scale and the first generation of their hybrids. The microsatellite primer TC111 is: F: 5'-CCATTTTGGTGCGTAGTA-3'; R: 5'-CTTGAGGGCACCTTTAG-3'. The method of the invention can quickly and accurately identify giant clams safflower, giant clams scales, and the first generation of hybrids of giant clams safflower × scale clams, which has important practical application value. At the same time, the invention has the advantages of simple method, intuitive and accurate results. Effective, not affected by the environment and developmental period, low cost and other advantages.

Description

technical field [0001] The invention belongs to shellfish molecular marker and hybrid species identification technology in aquatic biology technology, and specifically relates to a microsatellite primer, a kit and an identification method for identifying giant clam safflower, giant clam scale and their hybrid progenies. Background technique [0002] Tridacna is a large marine bivalve mollusc. It is not only delicious in meat and rich in nutrition, but also the shell can be made into exquisite shell carving art, which has extremely high ornamental value, collection value and medicinal value. In recent years, due to overfishing, giant clam resources have been severely damaged. For example, giant clam kushii is almost extinct in the South my country Sea, phosphorus-free giant clam is also extremely rare, and most other giant clam are also endangered. Breeding and value-added release is imminent. [0003] Tridacna crocea (Tridacna crocea) is a small species of giant clam, with a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2531/113C12Q2525/151C12Q2565/125
Inventor 马海涛喻子牛张跃环邓亚平肖述
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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