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Identification primers, kit and identification method for tridacna crocea, tridacna maxima and first filial generation of tridacna crocea and tridacna maxima

A technology of safranaceus and identification method, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Low cost, intuitive results, simple method effect

Pending Publication Date: 2021-09-03
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to its habit of living in tropical shallow transparent seas, it is very easy to be caught, especially since the 1960s and 1970s, with the popularization of diving equipment and motor boats, giant clam resources have been seriously damaged, even in the Some species have become extinct in many sea areas

Method used

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  • Identification primers, kit and identification method for tridacna crocea, tridacna maxima and first filial generation of tridacna crocea and tridacna maxima

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Embodiment 1

[0023] A small amount of mantle tissue was cut from the foot silk pores of Tridacna safranatum, Tridacna longis and their hybrids, and 40 individuals of each of 40 individuals of Tridacna safranus, Tridacna longis and their hybrids were extracted by phenol-chloroform extraction. Genomic DNA. Specifically, shred the mantle tissue fully, absorb the water with clean filter paper, put it into a 1.5mL centrifuge tube, then add 400μl lysate and 10μl proteinase K (10mg / ml), mix well on a shaker, and store at 50°C Digest in a water bath for 3 to 5 hours until the lysate is clear. Add an equal volume of saturated phenol (200 μL), chloroform / isoamyl alcohol (24:1) (200 μL) mixture and extract three times. Precipitate DNA with 1 mL of absolute ethanol, wash with 70% alcohol, dry at room temperature and dissolve in 100 μL of ultrapure water. The concentration and purity of DNA were detected by ultraviolet spectrophotometer, and the integrity of DNA was detected by 1% agarose gel electro...

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Abstract

The invention discloses identification primers, a kit and an identification method for tridacna crocea, tridacna maxima and a first filial generation of the tridacna crocea and the tridacna maxima. The identification primers are F:CCATTTTGGTGCGTAGTA and R:CTTGAGGGGCACCTTTTAG. According to the identification primers, the kit and the identification method, a pair of microsatellite primers TC111 which can be universally used in the tridacna crocea and the tridacna maxima and can be used for distinguishing two types of tridacna according to the size of an amplification band and have a clear band type, good repeatability and strong reliability is screened from transcriptome microsatellite primers of 150 pairs of the tridacna crocea; and the microsatellite primers TC111 have important application value in identification of individuals of the first filial generation of the tridacna crocea and the tridacna maxima.

Description

Technical field: [0001] The invention belongs to the field of identification of shellfish and its hybrids in aquatic biology technology, and in particular relates to an identification primer, a kit and an identification method for giant clams safranatum, giant clams and their hybrid progenies. Background technique: [0002] Giant clams are a kind of tropical large marine coral reef benthic shellfish. The species of giant clams distributed in the South China Sea mainly include giant clams of the genus Kushii, scaleless giant clams, scaled giant clams, long giant clams, and saffron giant clams. , Noah's clam, and Tridacna oysters of the genus Tridacna. Tridacna is a species of high economic value. It is the material basis of tropical marine life and fishery economy, providing resources such as aquarium ornamental, craft materials, and aquatic food. In addition, giant clams are also a major component of tropical island and reef ecosystems, providing habitats, protection areas,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125C12Q2525/151Y02A50/30
Inventor 马海涛喻子牛李军张跃环秦艳平
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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