Tridacna mitochondrion 16S gene amplification primer and application thereof

A gene amplification and mitochondrial technology, applied in the field of genetic engineering, can solve the problems of difficult identification and phylogenetic analysis of giant clam species
CN106399548APending Publication Date: 2017-02-15HAINAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN ¡ China
Patent Type
Applications(China)
Current Assignee / Owner
HAINAN UNIVERSITY
Publication Date
2017-02-15

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Abstract

The invention discloses a tridacna mitochondrion 16S gene amplification primer and application thereof. A universal forward primer F is designed on the basis of a tridacna 16S sequence in a Genbank database, specific reverse primers are designed aiming at different tridacna including Tri_squ_YZ_R (tridacna squamosa), Tri_max_YZ_R (tridacna maxima), Tri_gig_YZ_R (tridacna cookiana), Tri_cro-YZ_R (tridacna crocea) and Hip_hip_YZ_R (hippopus hippopus), PRC (polymerase chain reaction) is performed under a 50uL system, and finally types of tridacna can be identified through a strip of agarose gel electrophoresis. Tridacna species can be identified quickly and accurately by adopting the tridacna mitochondrion 16S gene amplification primer.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering and relates to a gene amplification primer, in particular to a giant clam mitochondrial 16S gene amplification primer and its application in identification of giant clam species. Background technique

[0002] The adductor muscles and feet of giant clams are very hypertrophic, and they are delicious and high-quality cooking ingredients; in traditional Chinese medicine, the shells of giant clams are considered to have the same curative effect as pearls; the ornamental value of giant clams mainly lies in two aspects , one is various shell carvings, bead chains, etc. that use shells as raw materials, and the other is live clams; similar to other shellfish, they can also produce pearls when they are occasionally parasitized or stimulated by foreign objects; popular in today's Buddhist circles Among the types of gemstones that can be used to exorcise evil spirits and ward off evil, the "Seve...

Claims

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