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Tridacna mitochondrion 16S gene amplification primer and application thereof

A gene amplification and mitochondrial technology, applied in the field of genetic engineering, can solve the problems of difficult identification and phylogenetic analysis of giant clam species

Pending Publication Date: 2017-02-15
HAINAN UNIVERSITY
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Problems solved by technology

[0004] Species identification and phylogenetic analysis of giant clams using morphological characteristics have become extremely difficult, especially for some closely related species, it is difficult to distinguish them using morphological characteristics alone

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  • Tridacna mitochondrion 16S gene amplification primer and application thereof
  • Tridacna mitochondrion 16S gene amplification primer and application thereof
  • Tridacna mitochondrion 16S gene amplification primer and application thereof

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Embodiment Construction

[0020] The screening method of the giant clam mitochondrial 16S gene amplification primer of the present invention adopts four steps: a. There are Tridacnaceae sequences, as shown in the sequence table; and primers are designed according to the Tridacnaceae sequence; b, the forward primer that can better amplify the 16S gene is selected by PCR; c, from the above-mentioned Tridacnaceae obtained In the tridacna sequence, find specific sequence fragments and design primers by comparison; d, use the designed specific primers to perform PCR to identify species of tridacna.

[0021] The present invention will be further described below in conjunction with embodiment:

[0022] 1. Sample collection: From 2015 to 2016, a total of 50 individuals of 2 genera and 6 species of giant clam were collected from the north and south coasts of China.

[0023] 2. Download the existing 16S sequences of five species of giant clam from the National Center for Biotechnology Information (NCBI, http: / / ...

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Abstract

The invention discloses a tridacna mitochondrion 16S gene amplification primer and application thereof. A universal forward primer F is designed on the basis of a tridacna 16S sequence in a Genbank database, specific reverse primers are designed aiming at different tridacna including Tri_squ_YZ_R (tridacna squamosa), Tri_max_YZ_R (tridacna maxima), Tri_gig_YZ_R (tridacna cookiana), Tri_cro-YZ_R (tridacna crocea) and Hip_hip_YZ_R (hippopus hippopus), PRC (polymerase chain reaction) is performed under a 50uL system, and finally types of tridacna can be identified through a strip of agarose gel electrophoresis. Tridacna species can be identified quickly and accurately by adopting the tridacna mitochondrion 16S gene amplification primer.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and relates to a gene amplification primer, in particular to a giant clam mitochondrial 16S gene amplification primer and its application in identification of giant clam species. Background technique [0002] The adductor muscles and feet of giant clams are very hypertrophic, and they are delicious and high-quality cooking ingredients; in traditional Chinese medicine, the shells of giant clams are considered to have the same curative effect as pearls; the ornamental value of giant clams mainly lies in two aspects , one is various shell carvings, bead chains, etc. that use shells as raw materials, and the other is live clams; similar to other shellfish, they can also produce pearls when they are occasionally parasitized or stimulated by foreign objects; popular in today's Buddhist circles Among the types of gemstones that can be used to exorcise evil spirits and ward off evil, the "Seve...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888
Inventor 周智唐佳俞小鹏黄勃陈国华王子元
Owner HAINAN UNIVERSITY
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