A universal single primer, kit and identification method suitable for the identification of common giant clam species in the southern sea area

Abstract

The invention discloses a universal single primer, a kit and an identification method suitable for identification of common clam species in southern sea areas. The present invention establishes a universal single-primer multiplex PCR that utilizes competitive annealing of multiple downstream primers to detect and identify 5 common species of Tridacnaceae in the southern sea area of ​​my country (phosphorus-free Tridacna, scaled clam, long clam, nova clam and oyster), and tested on actual samples, it has the advantages of simple method, intuitive results, accurate and effective, not affected by environment and growth period, and low cost.

Description

Technical field: [0001] The invention belongs to the technical field of molecular identification in aquatic organisms, and in particular relates to a PCR kit for quickly identifying common giant clam species in the southern sea area of ​​my country by using a universal single-primer multiplex PCR technology and an identification method thereof. Background technique: [0002] Tridacna belongs to Mollusca, Lamellibranchia, Veneroida, and Tridacnidae. It is a kind of tropical large marine coral reef benthic shellfish, and it is also the largest double shellfish in the ocean. shellfish. The biggest difference between Tridacna and other bivalve molluscs is that they have established a symbiotic relationship with zooxanthellae from the developmental metamorphosis to juvenile stage, and most of the nutrients needed for their growth come from the photosynthesis of zooxanthellae. This characteristic makes Tridacna Tridacna can directly use inorganic nutrients in tropical seas, which...

AI Technical Summary

Problems solved by technology

[0003] Adults of different giant clams are mainly identified by comparing the number and distribution of radial ribs on the shell, the number and distribution of prismatic scales, and the pattern of the mantle. However, shells have strong plasticity, and there are intermediate forms and intermediate habitats during the development process. , making it difficult to distinguish different species of Tridacnaceae

In addition, in the developmental stage of giant clam larvae, the morphological characteristics are very similar, making it more difficult to distinguish

At present, molecular biology mainly based on differences in COI and 16S rDNA mitochondrial gene sequences is used for identification of giant clam species, but the differences between these gene sequences are relatively small, and it is difficult to distinguish them by simple methods such as electrophoresis

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