Identification primer suitable for hippopus and common tridacna varieties in South China Sea and hybrids thereof and method thereof

A technology for hybrids and Tridacna, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as difficulty in identification of morphological characteristics and identification in the early stage of development, and achieve intuitive and accurate results. Simple method and low cost effect
CN110760595AActive Publication Date: 2020-02-07SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
5 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Publication Date
2020-02-07

Smart Images

  • Figure 1
    Figure 1
Patent Text Reader

Abstract

The invention discloses an identification primer suitable for hippopus and common tridacna varieties in South China Sea and hybrids thereof and a method thereof. The identification primer is a TC114 primer pair, and a forward primer and a reverse primer of the identification primer are as follows: F: 5'-AACACTGATTGGGCTCTT-3'R: 5'-TTGACGACGATGACGATA-3'. A pair of microsatellite primer TC114 primerpair is screened from 60 pairs of tridacna crocea microsatellite primers, can be universally used in hippopus and common tridacna varieties (tridacna derasa, tridacna squamosa, tridacna maxima, tridacna crocea and tridacna ovalvata) in South China, and can be distinguished according to the size of an amplified band, meanwhile, the microsatellite primer TC114 primer pair is clear in banding pattern, good in repeatability and high in reliability, and has potential application value in individual identification of hybrids between hippopus and common tridacna in South China Sea.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the shellfish molecular marker and species identification technology in the aquatic biology technology, and specifically relates to a method of using microsatellite primers to identify giant clam species and common species of giant clam in the South China Sea (scaleless giant clam, scaled giant clam, long clam, tomato The identification primers and methods of Tridacna red and Tridacna nova) and the potential application value of identifying hybrid individuals between them. Background technique

[0002] Tridacna is a kind of tropical large marine coral reef benthic shellfish. Common giant clam species distributed in the South China Sea mainly include scaleless giant clam, scaly giant clam, long giant clam, saffron giant clam, and nova giant clam. and giant oysters of the genus Giant oyster. Tridacna is a species of high economic value. It is the material basis of tropical marine life and fishery economy, providing resources suc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More