The invention discloses a method of calotropis gigantea L. mycorrhization container seedling breeding. The method comprises the following processes: treating seeds, preparing a substrate, carrying outmatrix inoculation, sowing the seeds, carrying out management in a seedling stage and taking seedlings out of a nursery. Qualified seeds are selected for disinfection treatment; then carrying out germination acceleration; after white is exposed on the seeds, the seeds are sowed into the pre-prepared matrix inoculated with a strain; and high-quality calotropis gigantea L. container seedlings are obtained through management and nursing in the seedling stage. According to the method provided by the invention, in order to solve the characteristics of slow growth, less developed root system, and easy infection of root rot and stem rot of the calotropis gigantea L. in a young seedling stage, the effects of root enrichment, growth promotion, seedling strengthening, and disease prevention of mycorrhiza are fully utilized, the quality of the calotropis gigantea L. seedlings is improved, the resistance of the seedlings is enhanced, the survival rate of the seedlings is improved and reaches 90%or above, the mycorrhiza has the capacity of self-fertilization, and the supplement of external fertilizers can be reduced in the seedling stage, so that the seedling breeding cost is reduced.