44 results about "Self-Fertilization" patented technology

The invention provides methods for chromosome doubling in plants. The technique overcomes the low yields of doubled progeny associated with the use of prior techniques for doubling chromosomes in plants such as grasses. The technique can be used in large scale applications and has been demonstrated to be highly effective in maize. Following treatment in accordance with the invention, plants remain amenable to self fertilization, thereby allowing the efficient isolation of doubled progeny plants.

Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants. In one such method, the homozygous recessive condition of male sterility causing alleles is maintained after crossing with a second plant, where the second plant contains a restoring transgene construct having a nucleotide sequence which reverses the homozygous condition. The restoring sequence is linked with a hemizygous sequence encoding a product inhibiting formation or function of male gametes. The maintainer plant produces only viable male gametes which do not contain the restoring transgene construct. Increase of the maintainer plant is also provided by self-fertilization, and selection for seed or plants which contain the construct.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants. In one such method, the homozygous recessive condition of male sterility causing alleles is maintained after crossing with a second plant, where the second plant contains a restoring transgene construct having a nucleotide sequence which reverses the homozygous condition. The restoring sequence is linked with a hemizygous sequence encoding a product inhibiting formation or function of male gametes. The maintainer plant produces only viable male gametes which do not contain the restoring transgene construct. Increase of the maintainer plant is also provided by self-fertilization, and selection for seed or plants which contain the construct.

Provided is a Korea and Mexico gulf scallop hybrid cultivation method. At the first year, good parent scallops are selected, maturation acceleration is performed on the parent scallops, ova of single Korea gulf scallop and sperms of single Mexico gulf scallop are collected and subjected to mixed insemination to establish Korea and Mexico gulf scallop first filial generation families, and a conventional gulf scallop breeding, keeping and offshore cultivation method is adopted to perform breeding; at the second year, good hybridization families are selected according to the phenotypes of the hybridization families obtained at the first year, good-phenotypic-trait individuals are selected from the good hybridization families, self-fertilization is performed for purification after maturation acceleration, and accordingly multiple Korea and Mexico gulf scallop first filial generation inbred families are established; the same method is adopted to continuously perform inbreeding for more than five generations, namely new variety of stable-inheritance, quickly-growing and large-sized scallops wide in temperature resisting range is obtained. The new variety simultaneously has the good advantages of the Korea gulf scallops and the Mexico gulf scallops. In addition, Korea and Mexico gulf scallop hybrids can be bred in most of sea areas in our country, the yield is improved by higher than 30% compared with the yield of Mexico gulf scallops bred in the southern of our country, and the Korea and Mexico gulf scallop hybrid cultivation method has great economic value.

The invention discloses a method of calotropis gigantea L. mycorrhization container seedling breeding. The method comprises the following processes: treating seeds, preparing a substrate, carrying outmatrix inoculation, sowing the seeds, carrying out management in a seedling stage and taking seedlings out of a nursery. Qualified seeds are selected for disinfection treatment; then carrying out germination acceleration; after white is exposed on the seeds, the seeds are sowed into the pre-prepared matrix inoculated with a strain; and high-quality calotropis gigantea L. container seedlings are obtained through management and nursing in the seedling stage. According to the method provided by the invention, in order to solve the characteristics of slow growth, less developed root system, and easy infection of root rot and stem rot of the calotropis gigantea L. in a young seedling stage, the effects of root enrichment, growth promotion, seedling strengthening, and disease prevention of mycorrhiza are fully utilized, the quality of the calotropis gigantea L. seedlings is improved, the resistance of the seedlings is enhanced, the survival rate of the seedlings is improved and reaches 90%or above, the mycorrhiza has the capacity of self-fertilization, and the supplement of external fertilizers can be reduced in the seedling stage, so that the seedling breeding cost is reduced.

The invention relates to a breeding method for fresh-eating red waxy hybrid maize belonging to the hybrid maize breeding field, comprising the procedures as follows: black pearl waxy corn with perfect plasmid and modena grain chosen as male parent and Darfur waxy corn with perfect plasmid and silvery white grain chosen as female parent are hybridized to establish an underlying group; recurrent selection is carried through in the underlying group in the aspects of red grain, waxy property, sweet taste, single plant production capacity, the ability of anti tan disease and lodging resistance, and repeated anisotropic hybridization and self-pollination are carried through to establish a hybridization group; the self-fertilization of parent strain and retrogression choiceness of the hybridization group are completed, vermeil hard grain is produced after 4-6 generations. The breeding method of the invention can breed the fresh-eating and eatable red waxy corn with high officinal value, good mouthfeel, high contents of natural haematochrome and rich nutrition which can be used in industry and as feed and has the advantages of strong ability to resist disease, drought resistance, flood resistance, high yield and fertility.

A method for breeding high-purity laver line includes decomposing the immature laver leaf by mussel protein extract liquor to generate its unicells or protoplasts, culturing or inducing chromosomal multiplication to obtain pure line of diploid filamnets, culturing until sexual mature, self fertilization to generate homozygous diploid filaments, and culturing the certified seeds or seedlings. Its advantages are excellent individual characteristics, and high yield and quality of laver.

The invention relates to a construction method and application of a male sterile line of argopecten irradians-argopecten purpuratus hybrid scallops. The male sterile line is a hybrid scallop line, wherein hybrid scallop line can not generate functional sperms, but the eggs can be fertilized and grow normally, progeny can inherit the male sterile characteristic, and the argopecten irradians-argopecten purpuratus hybrid scallops are the hybrid progeny of argopecten irradians eggs and argopecten purpuratus sperms. The construction method includes the steps that a great number of argopecten irradians-argopecten purpuratus hybrid first filial generation families are constructed, and male sterile individuals are found out from the argopecten irradians-argopecten purpuratus hybrid first filial generation families, then male parent argopecten purpuratus sperms of the male sterile individuals are used for continuous backcross selective breeding until all the backcross progeny are male sterile while the eggs are fertile, and the male sterile line of the argopecten irradians-argopecten purpuratus hybrid scallops can be obtained; the male sterile line and a maintainer line are hybridized to produce male sterile line parent scallops on a large scale, and the male sterile line parent scallops and a restorer line are hybridized to produce argopecten irradians-argopecten purpuratus hybrid scallop commodity seeds. The male sterile line of the scallops is set up in animals for the first time, and the obstacle to self-fertilization in hybridization of hermaphrodite scallops is effectively overcome.

The invention discloses a cultivation method of a transgenic rice sterile line based on a ZMAA1 gene. The cultivation method includes the following steps: A, obtainment of a rice AID1 gene expressioncassette; B, obtainment of an mVenus gene expression cassette; C, amplification of a pollen specific promoter Pg47; D, amplification of a corn pollen lethal gene ZMAA1; and E, linkage of all gene expression elements. The step E which is the linage of all the gene expression elements further includes the following steps: firstly, linking the complete rice AID1 gene to a pCAMBIA1301 vector; and secondly, introducing the mVenus gene expression element to the pCAMBIA1301 vector which is introduced with the AID1 gene in the first step. An F1 generation heterozygote follows the Mendelian separationlaw in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.

A method for preparing pure shellfish line includes such steps as choosing shellfish by shell height of 5-6 cm, putting them in water at 18-23 deg.C, feeding 5-6 times per day, changing all the water once every day, aerating with air up to visible microbuttle, controlling light irradiation by 400-500 Lux, effective accumulative temp. at 150-250 deg.C, artificially hastening parturition, drying sexually motured shellfish in air at 18-20 deg.C for 1-2 hr, putting in seawater at 24-25 deg.C, ultraviolet radiating by 300-800 mwh / L, obtaining female and male gametes in 0.5-1.5 hr, and self-fertilization.

The invention discloses a cross breeding method for eremochloa ophiuroides, comprising the choice of spica, manual detasseling, artificial supplementary pollination, the management after pollination and the seed harvest. The invention is characterized in that a technical cross breeding method for eremochloa ophiuroides is presented on the basis of the flowering habit and the property of certain self-fertilization fruitfulness rate. The red shank line of the eremochloa ophiuroides taken as female parent and the green shank line of the eremochloa ophiuroides taken as male parent are hybridized by utilizing the method to obtain the filial generation with green shank. The cross breeding method has simple manipulation and the filial generation with real, reliable and clear background, thereby providing an effective cross breeding method for the breeding of fine variety of eremochloa ophiuroides with various usages and providing an effective method for the usage of and the improvement of the abundant eremochloa ophiuroides in China.

The invention relates to a building method and application of an Argopecten irradians irradians and Argopecten purpuratus hybrid scallop maintainer line. The maintainer line allows the corresponding male sterility line offspring of the corresponding Argopecten irradians irradians and Argopecten purpuratus hybrid scallop to have male sterile and egg fertile features. The Argopecten irradians irradians and Argopecten purpuratus hybrid scallop is the hybrid offspring of the eggs of the Argopecten irradians irradians and the sperms of the Argopecten purpuratus. The method includes: building a large amount of Argopecten irradians irradians and Argopecten purpuratus hybrid scallop first filial generation families, and finding male sterile individuals, for male sterility line breeding, in the families; using the sperms of male parent Argopecten purpuratus for continuous back crossing, and respectively breeding a male sterility line-maintainer line combination with all the offspring being male sterile individuals; using the male sterility line to hybridize with the retainer line to produce male sterility line parent scallops, and using the male sterility line parent scallop produce Argopecten irradians irradians and Argopecten purpuratus hybrid scallop commercial fry. The method has the advantages that the scallop retainer line is built in animals for the first time, and the barrier of self-fertilization of the hermaphrodite scallops during hybridization is overcome effectively.

The invention relates to a construction method and application of a male sterility line of Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops. The male sterility line is a hybridization scallop strain according to which functional sperms cannot be generated, eggs of the male sterility line can be fertilized and grow normally and a male sterility characteristic of the male sterility line can be entailed on offsprings. The Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops are a filial generation between Argopecten irradians concentricus eggs and Argopecten purpuratus sperms. The method comprises the steps that first a large number of first filial generation families obtained after hybridization between Argopecten irradians concentricus and Argopecten purpuratus are constructed, and male sterile individuals are found among the first filial generation families obtained after hybridization between Argopecten irradians concentricus and Argopecten purpuratus; second, sperms of male parent Argopecten purpuratus of the male sterile individuals are used for continuous backcross for seed selection till backcross offsprings are all male and sterile and have fertile eggs, and the male sterility line of the Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops is obtained; third, male sterility line parent scallops are produced on a large scale through hybridization between the male sterility line and a maintainer line, and Argopecten irradians concentricus and Argopecten purpuratus hybridization scallop commercial offspring seeds are produced on a large scale through hybridization between the male sterility line parent scallops and a restorer line. According to the construction method and application of the male sterility line of the Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops, the male sterility line of scallops is constructed among animals for the first time, and the obstacle in self-fertilization of hybridization of hermaphrodite scallops is effectively removed.

The current invent relates to the construction and application of a three-line breeding system in the Peruvian scallop×bay scallop hybrids. The said three-line consists of a male sterile line, a maintainer line and a restorer line of the bay scallop×Peruvian scallop hybrids. Herein the Peruvian scallop×bay scallop hybrids are hybrids resulted from the eggs of the Peruvian scallops fertilized with sperm of the bay scallops. In constructing the three-line, male sterile individuals are selected from F1 inter-specific hybrid families and continuously backcrossed with sperm of the selling family of the sperm-providing bay scallops of the F1 hybrid to obtain a combination of male sterile line and maintainer line whose hybrid progenies are all male sterile. Then selected individuals from the male sterile line are continuously backcrossed with sperm from the selfing family of a bay scallop until the resulted progenies are all hermaphroditic and exhibit excellent production traits. Commercial male sterile brood stocks are produced by backcrossing the eggs of the male sterile line with the sperm of the maintainer line and commercial Peruvian scallop×bay scallop hybrid spats are produced by backcrossing the eggs of the male sterile line and sperm of the restorer line. This invent establishes the three-line breeding system in animals for the first time and efficiently overcomes the barrier of self-fertilization in the hybridization between hermaphroditic scallops. The yield of the hybrid scallops can be increased by over 50% compared with the bay scallops.

The invention discloses a breeding method for a golden yellow adductor muscle bay scallop new strain. New bay scallop products ''Zhong Ke Red'' and ''Zhong Ke NO.2'' serve as core collection groups, individuals with the adductor muscle being golden yellow are selected from the core collection groups as parent oysters so as to construct self-fertilization families, and the character of the golden yellow adductor muscle is subjected to rapid purification through family selection; the ''Zhong Ke Red'' self-fertilization family and ''Zhong Ke NO.2'' self-fertilization family are selected, wherein the ''Zhong Ke Red'' parent oysters and ''Zhong Ke NO.2'' parent oysters are the same in number, the hybridization group based on the two new products is constructed, the stability of the golden yellow character of the adductor muscle of the hybridization group is verified through breeding in different sea areas, and the golden yellow adductor muscle bay scallop new strain is obtained. The golden yellow character of the adductor muscle is rapidly purified through the family self-fertilization method, and the genetic diversity of the breeding strain is enriched by adopting a method of hybridization between varieties; and the nutritive value of the bay scallop is obviously increased, the product additional value and the breeding enterprise economic benefits are increased, and market prospects are wide.

The invention discloses a chemical method for effectively avoiding self-fertilization of tridacna. The method includes: allocating ID numbers to all tridacna subjected to induction, respectively placing into a sperm collection drum, timing from spermination in large quantity, obtaining sperms after spermination for 2-3min, filtering through a 500-mesh bolting silk screen to obtain filtrate which is sperm fluid, and marking +ID numbers ; moving the tridacna from the sperm collection drum into an ovum collection drum containing medicated bath liquid, wherein the liquid level of the medicated bath liquid is at least higher than a highest position of the tridacna; after ovulation for 15-20min, taking out the tridacna to obtain ovum cell liquid, filtering the ovum cell liquid through a 300-mesh bolting silk screen to obtain ovum cells on the bolting silk screen, cleaning the ovum cells on the bolting silk screen with seawater, and marking +ID numbers; checking sperm and ovum gamete quality, abandoning low-quality gametes, and subjecting the sperms and the ova different in source according to the different ID numbers to artificial fertilization as required.

Compositions and methods for the production of self-reproducing hybrid plants are provided. Compositions include suppression cassettes encoding polynucleotides and promoters that result in the MiMe diploid gamete phenotype compositions and suppression cassettes and expression cassettes useful for genome elimination of a parental diploid gamete in a fertilized zygote. The methods involve crossing a first plant comprising a first suppression cassette responsible for producing the MiMe diploid gamete phenotype and a first expression cassette expressing an active CENH3 mutant with a second plant comprising a second suppression cassette that reduces the level of wild-type CENH3 and a second expression cassette comprising a polynucleotide expressing CENH3 specifically in the ovule. Self fertilization of the resultant progeny plant results in the elimination of the female diploid genome in the zygote and normal development of the endosperm. Additionally provided are plants and seeds produced by the methods of the invention.

The invention relates to a cultivation method for a novel hybrid variety of female patinopecten caurinus and male patinopecten yessoensis. The method comprises the following steps: in a first year, ova of the female patinopecten caurinus and sperms of the male patinopecten yessoensis are separately obtained, and a female patinopecten caurinus x male patinopecten yessoensis hybrid population or family is established; after the scallops reach sexual maturity, the scallops with large individuals are separately selected from the above hybrid population or optimal hybrid family according to a ratioof female to male of 3:1, and self fertilization propagation expanding is separately performed in the population; and according to the same method, breeding is performed for consecutive four generations, so that the novel hybrid variety with good traits of the two scallops can be obtained. The novel variety provided by the invention grows fast, and has wide adaptability and strong stress resistance, an individual weight of the novel variety is 20% or more higher than that of the patinopecten yessoensis, and the survival rate is improved by about 10%; and the novel variety is suitable for being bred in sea areas of northern China such as Dalian Changhai and Shandong Changdao.

The invention discloses a breeding method for fireresistant and lodging resistant honglian cytoplasm male sterile lines. The manual detasseling method is utilized to pollinate and hybridize the female parent of 6069 and the male parent of yuetai B to obtain the F1 generation, and the F2 generation is obtained by self-fertilization of F1. The individual plant is chosen from the F2 generation, the short individual plant of strong replication ability is chosen to do testcross with yuetai A to obtain 67 combinations. The backcross is completed in the 67 combinations and corresponding maintenance line individual plant, the sterile anther and farina in didymous plant type are observed, six didymous plant type are chosen to finish backcross under high temperature conditions, wherein the sterile plant in the didymous type has stable sterility, indehiscent anther and 100 percent of anther abortion under high temperature conditions. The didiymous backcross is continuously carried through in the sterile plant of the didymous plants under high temperature conditions, fireresistant novel male sterile line yuetai A is obtained from the sixth generation, the testcross is carried through in the sterile line, thereby obtaining the lodging resistant variety of high quality and high yield utilized in production.

The invention discloses a cultivation method of a transgenic rice sterile line based on a UDT1 gene. The cultivation method includes the following steps: A, obtainment of an mKO gene expression cassette; B, obtainment of a rice UDT1 gene expression cassette; C, amplification of a wheat pollen lethal gene Ki; D, amplification of a gramineae plant pollen specific promoter Pg47; and E, linkage of allgene expression elements. The step E which is the linage of all the gene expression elements further includes the following steps: firstly, linking the mKO gene expression element to a pCAMBIA1301 vector; and secondly, introducing the complete rice UDT1 gene to the pCAMBIA1301 vector which is introduced with the mKO gene expression element in the first step. An F1 generation heterozygote followsthe Mendelian separation law in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.

The invention relates to a construction method and application of an argopecten irradians-argopecten purpuratus hybrid scallop restorer line. The restorer line is argopecten purpuratus which can recover the male fertile characteristic for male sterile line progeny, wherein argopecten irradians-argopecten purpuratus hybrid scallops are hybrid progeny of argopecten irradians eggs and argopecten purpuratus sperms. The construction method includes the steps that excellent individuals in a male sterile line are selected, the different argopecten purpuratus sperms are used for continuous backcross until the progeny are all hermaphrodite, and the restorer line of the male sterile line is obtained; finally, the restorer line is hybridized with the corresponding male sterile line of the argopecten irradians-argopecten purpuratus hybrid scallops to produce argopecten irradians-argopecten purpuratus hybrid scallop commodity seeds. The restorer line of the male sterile line is set up in animals for the first time, the obstacle to self-fertilization in hybridization of hermaphrodite scallops is effectively overcome, and the yield of the produced argopecten irradians-argopecten purpuratus hybrid scallops in the current year is larger than that of argopecten irradians and is increased by over 50%.

The invention discloses a cultivation method of a transgenic rice sterile line based on a TDR gene. The cultivation method includes the following steps: A, obtainment of a TagRFP gene expression cassette; B, amplification of a wheat pollen lethal gene Ki; C, obtainment of a rice TDR cassette; D, amplification of a pollen specific promoter Pg47; and E, linkage of all gene expression elements. The step E which is the linage of all the gene expression elements further includes the following steps: firstly, linking the TagRFP gene expression element to a pCAMBIA1390 vector; and secondly, introducing the complete rice TDR gene to the pCAMBIA1390 vector which is introduced with the TagRFP gene expression element in the first step. An F1 generation heterozygote follows the Mendelian separation law in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.

The invention discloses a breeding method for a golden yellow adductor muscle bay scallop new strain. New bay scallop products ''Zhong Ke Red'' and ''Zhong Ke NO.2'' serve as core collection groups, individuals with the adductor muscle being golden yellow are selected from the core collection groups as parent oysters so as to construct self-fertilization families, and the character of the golden yellow adductor muscle is subjected to rapid purification through family selection; the ''Zhong Ke Red'' self-fertilization family and ''Zhong Ke NO.2'' self-fertilization family are selected, wherein the ''Zhong Ke Red'' parent oysters and ''Zhong Ke NO.2'' parent oysters are the same in number, the hybridization group based on the two new products is constructed, the stability of the golden yellow character of the adductor muscle of the hybridization group is verified through breeding in different sea areas, and the golden yellow adductor muscle bay scallop new strain is obtained. The golden yellow character of the adductor muscle is rapidly purified through the family self-fertilization method, and the genetic diversity of the breeding strain is enriched by adopting a method of hybridization between varieties; and the nutritive value of the bay scallop is obviously increased, the product additional value and the breeding enterprise economic benefits are increased, and market prospects are wide.

Compositions and methods for the production of self-reproducing hybrid plants are provided. Compositions include suppression cassettes encoding polynucleotides and promoters that result in the MiMe diploid gamete phenotype compositions and suppression cassettes and expression cassettes useful for genome elimination of a parental diploid gamete in a fertilized zygote. The methods involve crossing a first plant comprising a first suppression cassette responsible for producing the MiMe diploid gamete phenotype and a first expression cassette expressing an active CENH3 mutant with a second plant comprising a second suppression cassette that reduces the level of wild-type CENH3 and a second expression cassette comprising a polynucleotide expressing CENH3 specifically in the ovule. Self fertilization of the resultant progeny plant results in the elimination of the female diploid genome in the zygote and normal development of the endosperm. Additionally provided are plants and seeds produced by the methods of the invention.

The invention discloses a cultivation method of a transgenic rice sterile line based on a wheat pollen lethal gene Ki. The cultivation method includes the following steps: A, amplification of the wheat pollen lethal gene Ki; B, obtainment of an mVenus gene expression cassette; C, obtainment of a rice gene expression cassette; D, amplification of a wheat pollen specific promoter Pg47; and E, linkage of all gene expression elements. The step E includes the following steps: firstly, introducing the wheat pollen lethal gene Ki to a pCAMBIA1300 vector; and then linking the mVenus gene expression element, a complete rice EAT1 gene and the wheat pollen specific promoter Pg47 to the pCAMBIA1300 vector. An F1 generation heterozygote follows the Mendelian separation law in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.

The invention discloses a cultivation method of a transgenic rice sterile line based on a CYP704B2 gene. The cultivation method includes the following steps: A, obtainment of an EYFP gene expression cassette; B, amplification of a wheat pollen lethal gene Ki; C, amplification of a wheat pollen specific promoter Pg47; D, obtainment of a rice gene expression cassette CYP704B2; and E, linkage of allgene expression elements. The step E which is the linage of all the gene expression elements includes the following steps: firstly, linking the EYFP gene expression element, the wheat pollen lethal gene Ki and the wheat pollen specific promoter Pg47 to a pCAMBIA1300 vector, and then introducing and linking a complete rice CYP704B2 gene to the composite vector. An F1 generation heterozygote followsthe Mendelian separation law in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.