Cultivation method of transgenic rice sterile line based on TDR gene

A technology of transgenic rice and cultivation methods, applied in the directions of genetic engineering, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of failure of seed production, insufficient stability of hybrid breeding, and unreliable combination.

Inactive Publication Date: 2018-12-07
QINGDAO YUANCE GRP CO LTD
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Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low
[0004] The genetic difference between the existing sterile lines in production is small, the fertility stability of hybrids is not enough, and t

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  • Cultivation method of transgenic rice sterile line based on TDR gene
  • Cultivation method of transgenic rice sterile line based on TDR gene
  • Cultivation method of transgenic rice sterile line based on TDR gene

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Embodiment Construction

[0034] The present invention is described in detail below in conjunction with embodiment. In order to make the object, technical solution and advantages of the present invention clearer and clearer, the present invention will be described in further detail below with reference to the accompanying drawings and examples, but the present invention is not limited to these examples.

[0035] In the invention, the common nuclear sterile gene TDR of rice is linked with the lethal gene Ki of wheat pollen, and at the same time, the orange fluorescent protein TagRFP gene is used as a reporter gene, and the rice TDR mutant is genetically modified to obtain F1 generation hybrids. The hybrids of the F1 generation follow Mendel's segregation law in the process of self-fertilization, and the offspring produced include heterozygotes that can maintain the triple-linkage genes and sterile sterile lines, which are the so-called third-generation sterile lines.

[0036] In order to solve the probl...

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Abstract

The invention discloses a cultivation method of a transgenic rice sterile line based on a TDR gene. The cultivation method includes the following steps: A, obtainment of a TagRFP gene expression cassette; B, amplification of a wheat pollen lethal gene Ki; C, obtainment of a rice TDR cassette; D, amplification of a pollen specific promoter Pg47; and E, linkage of all gene expression elements. The step E which is the linage of all the gene expression elements further includes the following steps: firstly, linking the TagRFP gene expression element to a pCAMBIA1390 vector; and secondly, introducing the complete rice TDR gene to the pCAMBIA1390 vector which is introduced with the TagRFP gene expression element in the first step. An F1 generation heterozygote follows the Mendelian separation law in a self-fertilization process, and produced progenies contain both the heterozygosis capable of maintaining three linkage genes and the sterile line without fertility.

Description

technical field [0001] The invention relates to the technical field of molecular biology nucleic acid chemistry, in particular to a method for preparing a genetically engineered sterile line of rice. Background technique [0002] Rice is the staple food of nearly half of the world's population. In addition to being edible, it can also be used for wine making, sugar refining and as an industrial raw material. Humans have a great demand for rice. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-generation hybrid rice is a three-line method using the nuclear-plasma interaction male sterile line as a geneti...

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Application Information

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IPC IPC(8): C12N15/82A01H5/00A01H6/46
CPCC12N15/8231C12N15/8289
Inventor 刘佳音米铁柱张国栋罗碧修旺珊万吉丽
Owner QINGDAO YUANCE GRP CO LTD
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