46results about How to "Rich genetic diversity" patented technology

The invention discloses a rice breeding method for polymerizing three disease resistance genes. The rice breeding method adopts combination of a molecular marker-assisted selection technology and an anther culture and conventional breeding technology to quickly polymerize a bacterial blight resistance gene Xa23, a rice blast resistance gene Pi-1 and a rice stripe virus resistance gene Stvb-i. The result shows that the disease resistance gene contained by an obtained anther culture material polymerizing the three disease resistance genes can carry out stable heredity and efficiently resist diseases. The rice breeding method creates a batch of new rice germplasm resources with stable resistance and provides a material basis for rice disease resistance and breeding.

The present invention provides a rice genome-wide breeding chip and its application. The rice genome-wide breeding chip of the present invention is Rice60K, which is a SNP chip made based on Infinium chip manufacturing technology. Each chip can simultaneously detect 24 samples, including 58,290 SNPs These marker sites have the DNA sequence shown in SEQ ID NO.1-58290. The chip can perform molecular marker fingerprint analysis on rice variety resources, perform genotype identification on offspring of hybrid populations, and identify variety authenticity. The analysis and screening of the genetic background of breeding materials and the association analysis of agronomic traits have broad application prospects.

The invention relates to a prawn low dissolved oxygen resistance family selection method, and belongs to the technical field of prawn stress-resistance trait selection. The method comprises the steps of base population establishing; F1 generation family establishing; same pool culturing; low dissolved oxygen resistance testing; F2 generation family establishing; continuous generation selecting, wherein different sources of parent prawns are selected to establish a full / half-slib family through directional mating, common environmental breeding is performed, 94 h low dissolved oxygen stress is performed, parent selecting and remaining are performed according to the low dissolved oxygen resistance test result and the growth measure result, after four generations of continuous selection, the family of distant genetic relationship is selected from the families with excellent growth performance and strong low dissolved oxygen resistance performance, the male and female prawns which are big in individual, strong in vigor and normal in gonad development are selected to serve as parent to breed as good varieties. According to the method, the problem occurring in prawn high-density culturing that the prawns are dead caused by the fact that the prawn growth is influenced by low dissolved oxygen is solved, and the development of the prawn high-density culture industry is greatly promoted.

The invention discloses application of miR1432 to rice drought stress and provides application of miR1432 or encoding genes thereof or recombinant vectors containing the encoding genes or expression cassettes containing the encoding genes or transgenic cell lines containing the encoding genes or recombinant bacteria containing the encoding genes or recombinant viruses containing the encoding genes to adjusting and controlling plant drought resistance. The nucleotide sequence of miR1432 is the sequence 1 in a sequence table. Experimental results show that transgenic rice is obtained by interfering in the expression of miR1432 in wild type rice, and compared with non-interference wild type rice, the transgenic rice resists drought stress. Thus, miR1432 can be used for rice high-drought-resistance breeding, and the genetic diversity of existing rice breeding materials is enriched.

The invention discloses astaxanthin synthetase of sphingomonas, an encoding gene of astaxanthin synthetase and a method for genetic manipulation of sphingomonas. The invention verifies that the sphingomonas can be used for producing astaxanthin, a biological synthesis way capable of producing the astaxanthin is contained, an MEP way and a carotenoid synthesis way are used for producing the astaxanthin in the sphingomonas, and the fact that in the sphingomonas, crtE and crtZ having relatively low homology with the known genes have the function of synthesizing the astaxanthin, can be further proven. Both the enzyme and the encoding gene thereof required by the astaxanthin biosynthetic way of the sphingomonas are not reported in the prior art, so that more resources are provided to the biosynthetic metabolism and transformation of the carotenoid. Genetic manipulation can be carried out on the sphingomonas by taking pBBR1MCS2 as a carrier and taking EGFP as a reporter gene, so that a foundation is laid for the genetic modification of the environmental microorganism of the type and the finding of the degradation mechanisms.

The invention discloses a rice male sterile protein and a coding gene and application thereof. The protein is one of 1) a protein formed by amino acid residue sequences of the SEQ ID No.2 in a sequence table or 2) a protein which is formed after one or more amino acid residues in the amino acid residue sequences of the SEQ ID No.2 in the sequence table are substituted and / or deleted and / or added and has the same activities as the amino acid residue sequences of the SEQ ID No.2. The rice male sterile protein gene can be used for breeding male sterile rice, and makes contributions for removing male hybrid rice.

A molecular marker-assisted selection method for cultivating a rice line with a medium amylose content. The method is: use the K81 rice line as a donor parent, and use a rice variety or line with excellent agronomic properties but not a medium amylose content as a recipient. After crossing, carry out 2-3 times of backcrossing, and then carry out 3-5 times of self-crossing; at the same time, the donor parent and recipient parent are planted in each generation. In each generation of selection, the molecular marker RA19 was used for assisted selection, and the contemporary individual plants containing the Wx genotype of the donor parent were selected as the individual plants of the next backcross or self-cross, and the second and subsequent self-cross plants In the selection, the amylose content was also determined for selection. The method can quickly and effectively select rice materials with medium amylose content, reduce breeding scale, reduce breeding cost, efficiently improve rice amylose content, and rapidly cultivate rice lines with medium rice amylose content.

The invention discloses a wheat yield and quality synergistically improving selection breeding method of the fittest. The invention provides a method for breeding target wheat varieties with improved yield and quality, which combines the advantages of the pedigree method and the hybrid method, and provides a whole-generation final selection using molecular markers combined with phenotypic identification, and each generation is selected according to different standards The selected method is different from the derivative system method, which not only retains the traceability of the lineage history and genetic relationship of the pedigree method, but also retains the characteristics of simple operation and rich genetic diversity of the hybrid method. The background of the parents before the combination is clear, the purpose of the combination is strong, the selection process is simple, the goal is clear, and it is easy to operate, especially for those who have no breeding experience, it is easier to master, providing technical support for the rapid selection of high-quality and high-yielding new wheat varieties.

The invention discloses a litchi pollen collecting and storing method, which belongs to the technical field of fruit tree pollination. The method comprises the following steps: 1, collecting litchi anthers which are matured but not cracked at a developmental stage and putting the collected litchi anthers into a glass culture dish; 2, putting the glass culture dish in which the litchi anthers are placed into a drying box and drying the litchi anthers in the glass culture dish by using the drying box; 3, pouring the dried litchi anthers and the scattered pollen into a stainless steel screen, and putting the pollen into a centrifuge tube after enabling the pollen to pass through the stainless steel screen; and 4, sealing the pollen in the centrifuge tube and storing the pollen in an ultra-low temperature refrigerator at -86 DEG C. The method is simple, convenient and easy to implement, and can be used for performing a collecting operation even under different weather conditions. The obtained pollen is high in germination rate and can be stored for the longest time.

The invention discloses a method for ecologically restoring heavy metal contaminated soil. According to the method, heavy metals are absorbed by a plant growth regulator, an artificial chelating agent and bamboo willows in a combining manner. The method disclosed by the invention can be used for absorbing heavy metal ions, namely Pb, Cu, Zn, Cd and Cr, by utilizing the high-quality bamboo willows which is fast in growth, high in resistance and good in material and accelerating the absorption of the bamboo willows on the heavy metal ions by adopting the plant growth regulator and the artificial chelating agent, so that the purpose of ecological restoration for 3-4 years can be achieved; after the heavy metal contaminated soil is ecologically restored through the bamboo willows, the concentration of the Pb, the Cu, the Zn, the Cd and the Cr which are contained in the soil can accords to the II class standard of the 'Environmental quality standard for soils (GB15618-1995)'.