Rice male sterile protein and coding gene and application thereof

A technology for encoding genes and proteins, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as inability to achieve three-line matching and limited application

Active Publication Date: 2013-11-13
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the segregation ratio of fertile and sterile in the F1 generation is 1:1, dominant genic sterile rice usually has neither restorer nor maintainer lines, so it cannot be directly used for production; Sexual genic male sterility and polygenic recessive genic male sterility. It is difficult to find corresponding maintainer lines for these types of sterile lines in production, and it is impossible to achieve three-line matching, which limits their application in the use of heterosis.

Method used

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  • Rice male sterile protein and coding gene and application thereof
  • Rice male sterile protein and coding gene and application thereof
  • Rice male sterile protein and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040]Embodiment 1, the phenotype and genetic analysis of rice male sterile mutant mspl

[0041] 1. Phenotype analysis of rice male sterile mutant mspl

[0042] The rice male sterile mutant mspl is a natural mutant of Zhongxian 3037 (WT, purchased from the Agricultural College of Yangzhou University) found in the field by our laboratory. There was no significant difference between the mspl mutant and the control 3037 in the vegetative growth stage, and the mutant showed a typical male sterile phenotype after heading, which showed that the anthers were white and would not dehisce and release pollen. The results of semi-thin sections showed that there was no pollen in the anthers of the mutant, parenchyma cells of similar size and shape were distributed in the anthers, some anthers were even lignified in the center, and there were tissue differentiations similar to vascular bundles ( figure 1 ).

[0043] We granted the mspl mutant to the pollen of the wild type, and found that...

Embodiment 2

[0048] Embodiment 2, the acquisition of MSPL and its coding gene MSPL

[0049] 1. Map-based cloning of the genomic gene of MSPL

[0050] In order to clone the MSPL gene, we will cross the homozygous male sterile mutant mspl and Nipponbare and obtain the F 1 F 2 In the population, 236 F2 recessive individuals (F2 individuals with male sterility phenotype) were initially mapped for the MSPL gene. Using STS (Sequence-Tagged Site) molecular markers and PCR method, we found that the STS markers S1, S2, S3 and S4 on chromosome 7 had obvious linkage with the mutation site. Most of the exchanged plants between the mutation site and S2 were also exchanged between the mutation site and S1, and most of the exchanged plants between the mutation site and S3 were included in the mutation site and S4 in exchange among individual plants. At the same time, the exchanged individual plants between the mutation site and S3 were different from those between the mutation site and S2, so it was ...

Embodiment 3

[0059] Embodiment 3, complementation experiment of male sterile mutant MSPL phenotype

[0060] 1. Construction of complementary vector pCMSPL and complementary control vector pCMSPLC

[0061] BAC OsJNBa0057C01 (purchased from Shanghai National Gene Research Center, Chinese Academy of Sciences, No. OsJNBa0057C01) was digested with EcoRI to obtain the full length of 3870 bases upstream of the start codon ATG of MSPL and 1306 bases after the stop codon TGA The DNA fragment (5360bp) of the sequence was cloned into the EcoRI site of pCAMBIA1300 (DingGuo, MCV033), and the complementary expression vector pCMSPL was constructed. The constructed complementary vector pCMSPL was digested with SacI to remove part of the promoter region, the entire coding region and the 3' regulatory region of the MSPL gene, and retain the part of the promoter region at the 5' end, thus constructing the complementary control vector pCMSPLC( figure 2 Middle C).

[0062] 2. Acquisition and phenotypic iden...

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Abstract

The invention discloses a rice male sterile protein and a coding gene and application thereof. The protein is one of 1) a protein formed by amino acid residue sequences of the SEQ ID No.2 in a sequence table or 2) a protein which is formed after one or more amino acid residues in the amino acid residue sequences of the SEQ ID No.2 in the sequence table are substituted and / or deleted and / or added and has the same activities as the amino acid residue sequences of the SEQ ID No.2. The rice male sterile protein gene can be used for breeding male sterile rice, and makes contributions for removing male hybrid rice.

Description

technical field [0001] The invention relates to rice male sterility protein and its coding gene and application. Background technique [0002] Rice is one of the most important food crops for human beings, and about half of the world's population uses rice as the main food. China's rice cultivation area accounts for about 23% of the world's total, and its rice output accounts for about 37% of the world's total. The sown area of ​​rice in China accounts for 30% of the national sown area of ​​grain crops, but the total output accounts for more than 42% of the grain crop output, and the yield per unit area is 45.7% higher than that of the entire grain crop on average. One of the important reasons is the heterosis widely used. Heterosis is a common phenomenon in the biological world. It refers to the hybridization of two genetically different parents. Hybrid rice shows significant heterosis in many traits, comprehensively manifested in nutritional advantage, reproductive adva...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N7/01
Inventor 程祝宽洪丽兰李明唐丁王克剑
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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