A method of selection and breeding of the inferior for synergistic improvement of wheat yield and quality
A yield and wheat technology, applied in botany equipment and methods, biochemical equipment and methods, seed and rhizome treatment, etc., can solve problems such as difficult to adapt to the current breeding situation, achieve rich genetic diversity, clear parental background, and simple operation Effect
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[0063] Embodiment 1, a kind of breeding method of the wheat that yield and quality improve
[0064] The following two combinations are used as an example to filter:
[0065] Combination 1: Combination of Ji 954072 and Jinan 17
[0066] Combination 2: the combination of Jimai 954072 and Jimai 19.
[0067] 1. Breeding method of wheat with improved yield and quality
[0068] 1. Identify whether the parental combination contains proteins related to yield or quality
[0069] 1) Protein level detection
[0070] Extraction of glutenin from tissues of Ji 954072, Jinan 17 and Jimai 19 (Ji Jun, Liu Dongcheng, Wang Jing, et al. A method for extracting high and low molecular weight glutenin subunits from wheat[J]. Genetics, 2008, 30( 1):123-126.), SDS-PAGE detection.
[0071] Chuanmai 56 and Chinese spring were used as standard controls.
[0072] The SDS-PAGE electrophoresis results of HMW-GS of Ji 954072, Jinan 17 and Jimai 19 are as follows figure 1 Shown, from left to right: Chu...
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