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Litchi pollen collecting and storing method

A preservation method and technology for litchi pollen, which are applied in the fields of plant preservation, botanical equipment and methods, and applications, can solve the problems of difficulty in pollination and fertilization of litchi varieties, and achieve rich genetic diversity, high germination rate, and rich combination range. Effect

Inactive Publication Date: 2014-04-16
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for collecting, drying and preserving litchi pollen, which can be used for artificial pollination in the second year without changing the germination rate after one year of storage, so as to solve the problem of difficult pollination and fertilization of litchi varieties caused by reasons such as poor natural environment, Meet the needs of pollination, especially artificial pollination of early-maturing varieties, and have important application value for litchi germplasm innovation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the collection preservation method of fine day

[0033] (1) Collection of anthers

[0034] Standards for picking anthers: only pick mature and uncracked anthers, that is, the anthers in the second to third stages of male flower development, that is, the filaments are half or fully elongated but the anthers are not cracked. Hands or tweezers should be used lightly during the picking process to minimize mechanical damage to the anthers.

[0035] (2) Drying of anthers

[0036] Put the picked anthers into a glass petri dish and put them in a drying oven. First, set the temperature at 40°C and dry for 2 hours, then set the temperature at 35°C and dry for 1 hour, and finally set the temperature at 27°C to 28°C and dry for 1-2 hours. 2 hours. It is advisable that the anthers be packed in the thickness of the glass petri dish within 2 layers.

[0037] (3) Preservation of pollen

[0038] Pour the dried anthers and scattered pollen from the glass petri dish int...

Embodiment 2

[0039] Embodiment 2: the collection preservation method of cloudy and rainy day

[0040] (1) Collection of anthers

[0041]Standards for picking anthers: only pick mature and uncracked anthers, that is, the anthers in the second to third stages of male flower development, that is, the filaments are half or fully elongated but the anthers are not cracked.

[0042] When picking anthers on cloudy and rainy days, it is necessary to shake the flowers in advance to shake off the water droplets from the flower spikes as much as possible. Hands or tweezers should be used lightly during the picking process to minimize mechanical damage to the anthers. The picked anthers are first poured on the absorbent paper to absorb water, and the water on the surface of the anthers is absorbed by the absorbent paper, and then placed in a drying oven to dry in time.

[0043] (2) Drying of anthers

[0044] Put the picked anthers into a glass petri dish and put them in a drying oven. First, set the...

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Abstract

The invention discloses a litchi pollen collecting and storing method, which belongs to the technical field of fruit tree pollination. The method comprises the following steps: 1, collecting litchi anthers which are matured but not cracked at a developmental stage and putting the collected litchi anthers into a glass culture dish; 2, putting the glass culture dish in which the litchi anthers are placed into a drying box and drying the litchi anthers in the glass culture dish by using the drying box; 3, pouring the dried litchi anthers and the scattered pollen into a stainless steel screen, and putting the pollen into a centrifuge tube after enabling the pollen to pass through the stainless steel screen; and 4, sealing the pollen in the centrifuge tube and storing the pollen in an ultra-low temperature refrigerator at -86 DEG C. The method is simple, convenient and easy to implement, and can be used for performing a collecting operation even under different weather conditions. The obtained pollen is high in germination rate and can be stored for the longest time.

Description

technical field [0001] The invention belongs to the technical field of fruit tree pollination, and more specifically relates to a method for collecting and preserving litchi pollen. Background technique [0002] Litchi is the most important fruit in the four provinces of Guangdong, Guangxi, Hainan and Fujian. At present, there are two major problems in the litchi industry: irrational variety structure and very concentrated maturity period, and variety innovation is an indispensable and important link to solve these two problems. For a long time, the innovation of litchi varieties has been mainly realized through seed selection. Due to the long-term adoption of grafting and vegetative propagation methods, the planting of a single variety, and the loss of many germplasm resources due to land expropriation, the genetic diversity of litchi in production areas has been continuously lost, and it is difficult to realize the innovation of litchi varieties through seed selection. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00A01H1/02
Inventor 欧良喜王丽敏陈洁珍蔡长河付丹文张春阳王晓容
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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