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Hibiscus cannabinus lead stress response gene EST-SSR primer group and kit

A primer set and kit technology, applied in the biological field, can solve problems such as gene function loss and trait variation

Pending Publication Date: 2021-03-26
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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Problems solved by technology

Therefore, expressed sequence tag simple sequence repeat polymorphism (EST-SSR) molecular markers may directly cause gene function defects. If the gene in which it is located is a gene that controls important agronomic traits, the SSR sequence variation in the exon region of the gene may cause Loss of gene function, resulting in variation in traits

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  • Hibiscus cannabinus lead stress response gene EST-SSR primer group and kit
  • Hibiscus cannabinus lead stress response gene EST-SSR primer group and kit
  • Hibiscus cannabinus lead stress response gene EST-SSR primer group and kit

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Embodiment Construction

[0037] The EST-SSR markers of key genes in kenaf response to lead stress are the basis for the collection of kenaf transcriptome sequencing data and kenaf materials, SSR marker development, GO and KEGG enrichment analysis, differential gene-protein interaction network analysis, genomic DNA extraction, SSR typing, genetic diversity analysis, genetic structure analysis and other processes ( figure 1 ).

[0038] The present invention utilizes kenaf transcriptome sequencing data to obtain the key gene of lead stress to develop kenaf EST-SSR primer specific method is:

[0039] 1. Kenaf transcriptome sequencing data and material collection

[0040] The transcriptome data are in NCBI SRA:SRR9163842.to SRR9163845. The kenaf germplasm resources and their geographical sources used in this study are shown in Table 1. There are a total of 138 kenaf germplasms, including 30 from 9 provinces in China, Pakistan 2, France 2, Cuba 4, Ghana 2, zimbabwe 3, USA 19, South Africa 2, Sudan 5, Thai...

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Abstract

The invention discloses a Hibiscus cannabinus lead stress response gene EST-SSR primer group and a kit. The invention provides primer sequences of 22 Hibiscus cannabinus EST-SSR markers. The 22 EST-SSR markers have polymorphism in 138 Hibiscus cannabinus varieties / strains, Hibiscus cannabinus germplasms can be completely distinguished, and 138 materials are divided into three groups, which indicates that the Hibiscus cannabinus germplasms have obvious grouping ability to resist lead stress, so that a candidate marker is provided for screening Hibiscus cannabinus germplasm resources resistant to heavy metal lead stress and heavy metal lead resistant molecular marker-assisted breeding.

Description

technical field [0001] The invention relates to an EST-SSR marker, in particular to an EST-SSR primer set and a kit for a kenaf lead stress response gene, and belongs to the field of biotechnology. Background technique [0002] Kenaf is an important bast fiber crop, raw material for hemp spinning, and one of the important raw materials for green and environmentally friendly natural fiber products. Kenaf can enrich heavy metals in soil and play an important role in repairing acidic soil. The research on kenaf resources still stays in the description of botanical traits, agronomic traits, and quality traits, and there is a lack of research on stress resistance traits at the molecular level. The development of molecular markers of functional genes can speed up the screening process of heavy metal-resistant kenaf materials and reduce the workload and time of parental selection. [0003] Simple sequence repeats polymorphism (Simple sequence repeats, SSR) is one of the represent...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 安霞金关荣柳婷婷李文略骆霞虹陈常理朱关林
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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