Molecular marker of multieffect QTLs locus qTLS-4 for regulating and controlling size of rice leaf and application of molecular marker

A technology of leaf size and molecular markers, which is applied in the fields of rice breeding and molecular biology, can solve the problems of relatively few studies on molecular genetic mechanisms, and achieve the effects of good marker polymorphism, accelerated breeding, and accurate QTL positioning

Active Publication Date: 2020-12-25
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are relatively few studies on leaf shape traits, the complex regulatory network formed by the combination of various pleiotropic genes, and related molecular genetic mechanisms.

Method used

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  • Molecular marker of multieffect QTLs locus qTLS-4 for regulating and controlling size of rice leaf and application of molecular marker
  • Molecular marker of multieffect QTLs locus qTLS-4 for regulating and controlling size of rice leaf and application of molecular marker
  • Molecular marker of multieffect QTLs locus qTLS-4 for regulating and controlling size of rice leaf and application of molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, acquisition of experimental materials

[0063] Huazhan was used as the donor parent, and the local rice variety Reyan was used as the recipient parent to construct RILs by crossing, using the single-seed method (i.e., bagging a single plant of F1 until the phenotype of the offspring did not occur. segregation), finally got 134 stable genetic lines, such as figure 1 .

[0064] Select 60 seeds (F13) of the parent and each strain, soak the seeds for 2 days after surface disinfection, wrap them in a moist towel, put them in a 37°C incubator for 48 hours, and select the seeds with consistent dew and whiteness for sowing. After 30 days, the parents with similar growth conditions and 24 seedlings of each line were selected for transplantation. All rice materials were planted in the experimental field of the School of Chemistry, Zhejiang Normal University, Jinhua City, Zhejiang Province, and were managed routinely.

Embodiment 2

[0065] Embodiment 2, blade size data measurement

[0066] At the tillering stage, 5 tillers were randomly selected for each line (transplanted parents, line seedlings), and the leaf length, leaf width, and leaf area of ​​flag leaves were measured respectively. Record the data of each group and calculate the average value.

[0067] figure 2 A. image 3 A. Figure 4 In A, RY represents the rice variety Reyan, and HZ represents the rice variety Huazhan; according to figure 2 A. image 3 A. Figure 4 A. It can be known that the data of leaf length, leaf width and leaf area of ​​sword blade are continuous normal distribution and wide range, there are many super-parents, showing the genetic characteristics of quantitative traits.

Embodiment 3

[0068] Embodiment 3, QTL location analysis

[0069] Use the genetic map constructed by a large number of SNP and Indel markers developed in the laboratory to map the quantitative trait locus (QTL) interval of rice leaf size, and analyze the markers and quantitative trait phenotype values ​​of the entire chromosome group through R-QTL professional software QTLs were mapped to corresponding positions in the linkage group one by one, and their genetic effects were estimated. If a molecular marker with LOD>3 is detected, it is considered that there is a QTL between the two markers corresponding to the highest LOD value. A pleiotropic QTL located between the Indel Fls-1 marker and the Indel Fls-2 marker on chromosome 4 was found in the entire genome, with leaf length LOD values ​​as high as 6.92, leaf width LOD values ​​as high as 4.51, and leaf area LOD values ​​as high as 6.37( figure 2 B. image 3 B. Figure 4 B). Its genetic distance is 87.1-97.4cM, physical distance is 2...

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Abstract

The invention discloses a multieffect QTLs locus for regulating and controlling a size of a boot leaf of rice. The QTLs locus is located on a DNA fragment of a chromosome 4# of the rice, is called after qTLS-4, has a genetic distance of 87.1cM-97.4cM and a physical distance of 28747360bp-29507404bp and plays a role in regulating and controlling the size of the boot leaf of the rice. The inventionfurther discloses a molecular marker of the multieffect QTLs locus qTLS-4 for regulating and controlling the size of the boot leaf of the rice. The molecular marker comprises two pairs of molecular markers Indel Fls-1 and Indel Fls-2 which are closely chained. According to the molecular marker, whether a rice variety or line has the QTLs for regulating and controlling the size of the boot leaf ofthe rice or not is detected by using a molecular marker method, and thus, a breeding progress of excellent varieties of the rice is accelerated.

Description

technical field [0001] The invention belongs to the technical field of rice breeding and molecular biology, and specifically relates to a molecular marker of a pleiotropic QTLs locus qTLS-4 regulating rice leaf size and its application. Background technique [0002] Rice (Oryza sativa.L) is the food crop with the largest planting area, the largest total output, and the highest consumption in my country. Rice breeding in China has mainly experienced dwarf breeding, three-line hybrid rice cultivation, two-line hybrid rice cultivation, and inter-subspecies breeding. Six important processes including heterosis utilization, ideal plant type breeding and green super rice cultivation [1,2] . In the breeding process, a good individual plant type is a necessary condition for high yield of rice, and leaf shape is an important part of plant shape. Many breeders have proposed an ideal plant type model for high yield of rice, and they all mentioned the leaf shape without exception. bree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 饶玉春胡娟王跃星王盛李梦佳潘晨阳马若盈金佳怡乐巧娜俞淑颖
Owner ZHEJIANG NORMAL UNIVERSITY
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