Detection method for goat CSN1S1 gene insertion/deletion, and applications thereof

A detection method, goat technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem of lack of research on the correlation between CSN1S1 gene genetic variation and reproductive traits, and achieve low-cost effects

Inactive Publication Date: 2017-09-15
NORTHWEST A & F UNIV
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Problems solved by technology

[0007] At present, the research on CSN1S1 gene mainly focuses on the research on traits related to lactation and milk...

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  • Detection method for goat CSN1S1 gene insertion/deletion, and applications thereof
  • Detection method for goat CSN1S1 gene insertion/deletion, and applications thereof
  • Detection method for goat CSN1S1 gene insertion/deletion, and applications thereof

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[0029]The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, which are explanations rather than limitations of the present invention.

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Abstract

The invention discloses a detection method for goat CSN1S1 gene insertion/deletion, and applications thereof. According to the method, the whole genomic DNA of a goat to be detected is used as a template, the goat CSN1S1 gene is amplified through a PCR technology, and agarose gel electrophoresis is performed to identify whether the indel polymorphism exists in the goat CSN1S1 gene. According to the present invention, the large sample (N is more than 1000) related analysis results reveal that the different types of the CSN1S1 gene indel are significantly related to the first lambing trait of Shanbei white cashmere goat, such that the different types of the CSN1S1 gene indel can be used as the DNA mark for improving the first lambing trait of the goat; and the method for rapidly detecting the goat CSN1S1 gene indel can be used in the marker-assisted selection breeding of goat reproductive traits in China, and is conducive to the rapid establishment of the goat genetic resource groups having the high lambing number trait.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the detection of gene insertion / deletion (indel), in particular to the rapid and accurate detection of the 11-bp insertion / deletion (indel) of goat CSN1S1 gene NC_030813.1:g.6654-6664 method and its application. Background technique [0002] Animal breeding technology mainly includes conventional breeding technology based on phenotype and phenotypic value and molecular breeding technology based on DNA polymorphism. As an important part of the molecular breeding technology system, marker-assisted selection (MAS) breeding technology first detects DNA polymorphisms of important genes, then analyzes the correlation between DNA polymorphisms and genetic traits, and finally Trait selection based on DNA markers significantly associated with genetic traits. As a new technology born during the development of modern molecular biology technology, MAS breeding technology c...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 闫海龙王珂潘传英屈雷朱海鲸陈宏蓝贤勇
Owner NORTHWEST A & F UNIV
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